LOCUS       sequence-221326-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses yeast Spt2-GFP fusion protein mutated from lysine to 
            arginine at site 166.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Rossl A, Denoncourt A, Lin MS, Downey M
  TITLE     A synthetic non-histone substrate to study substrate targeting by 
            the Gcn5 HAT and sirtuin HDACs.
  JOURNAL   J Biol Chem. 2019 Apr 19;294(16):6227-6239. doi: 
            10.1074/jbc.RA118.006051. Epub 2019 Feb 25.
  PUBMED    30804216
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.006051"; journalName: "J Biol Chem"; date: 
            "2019-04-19- 19"; volume: "294"; issue: "16"; pages: "6227-6239"
COMMENT     Sequence Label: Spt2-K166R-GFP
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     633..653
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttcactggag ttgtcccaat tcttgttgaa ttnnntggtg atgttaatgg gcacaaattt
       61 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
      121 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt cacttatggt
      181 gttcaatgct tttcaagata cccagatcat atgaaacnnc nnnacttttt caagagtgcc
      241 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
      301 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
      361 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca
      421 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt
      481 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca
      541 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt
      601 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct
      661 gggattacac atggcatgga tgaactatac aaatagggcg cgccacttct aaataagcga
      721 atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca
      781 aatt
//
