LOCUS       sequence-221327-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses yeast Spt2-GFP fusion protein mutated from lysine to 
            arginine at site 166.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Rossl A, Denoncourt A, Lin MS, Downey M
  TITLE     A synthetic non-histone substrate to study substrate targeting by 
            the Gcn5 HAT and sirtuin HDACs.
  JOURNAL   J Biol Chem. 2019 Apr 19;294(16):6227-6239. doi: 
            10.1074/jbc.RA118.006051. Epub 2019 Feb 25.
  PUBMED    30804216
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.006051"; journalName: "J Biol Chem"; date: 
            "2019-04-19- 19"; volume: "294"; issue: "16"; pages: "6227-6239"
COMMENT     Sequence Label: Spt2-K166R-GFP
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     21..37
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     22..38
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(60..225)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     146..170
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(289..309)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 agctggtacc gggccccccc tcgaggtcga cggtatcgat gtagaggtgt ggtcaataag
       61 agcgacctca tactatacct gagaaagcaa cctgacctac aggaaagagt tactcaagaa
      121 taagaatttt cgttttaaaa cctaagagtc actttaaaat ttgtatacac ttattttttt
      181 tataacttat ttaataataa aaatcataaa tcataagaaa ttcgcttatt tagaagtggc
      241 gcgccctatt tgtatagttc atccatgcca tgtgtaatcc cagcagctgt tacaaactca
      301 agaaggacca tgtggtctct cttttcgttg ggatctttcg aaagggcaga ttgtgtggac
      361 aggtaatggt tgtctggtaa aaggacaggg ccatcgccaa ttggagtatt ttgttgataa
      421 tggtctgcta gttgaacgct tccatcttca atgttgtgtc taattttgaa gttaactttg
      481 attccattct tttgtttgtc tgccatgatg tatacattgt gtgagttata gttgtattcc
      541 aatttgtgtc caagaatgtt tccatcttct ttaaaatcaa taccttttaa ctcgattcta
      601 ttaacaaggg tatcaccttc aaacttgact tcagcacgtg tcttgtagtt cccgtcatct
      661 ttgaaaaata tagttctttc ctgtacataa ccttcgggca tggcactctt gaaaaagtca
      721 tgccgtttca tatgatctgg gtatcttgaa aagcattgaa caccataagt gaaagtagtg
      781 acaagtgttg gccatggaac aggtagtttt ccagtagtgc aaataaattt aagggtaagt
      841 tttccgtatg ttgcatcacc ttca
//
