LOCUS       sequence-22134-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Duennwald ML, Jagadish S, Muchowski PJ, Lindquist S
  TITLE     Flanking sequences profoundly alter polyglutamine toxicity in yeast.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50.
  PUBMED    16832050
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 18. 103(29):11045-50."
COMMENT     Sequence Label: GPD 104QDProGFP p416
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(441..457)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(441..457)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(546..569)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 ccggagaaag ctcctcgccc ttgctcacca gggnatcccc cgggctgcag ttgttgctgt
       61 tgctgctgtt gttgctgttg ctgctgttgt tgctgttgct gctgttgttg ctgttgctgc
      121 tgttgttgct gttgctgctg ttgttgctgt tgctgctgtt gttgctgttg ctgctgttgt
      181 tgctgttgct gctgttgttg ctgttgctgc tgttgttgct gttgctgctg ttgttgctgt
      241 tgctgctgtt gttgctgttg ctgctgttgt tgctgttgct gctgttgttg ctgttgctgc
      301 tgttgttgct gttgctgctg ttgttgctgt tgctgctgtt gttgctgttg ctgctgttgg
      361 aagcttttga gggactcgaa ggccttcatc agcttttcca gggtcgccat ggcggtcgac
      421 ctcnaggggg ggcccggtac gatccactag ttctagaatc cgtcgaaact aagttctggt
      481 gttttaaaac taaaaaaaag actaactata aaagtagaat ttaagaagtt taagaaatag
      541 atttacagaa ttacaatcaa tacctaccgt ctttatatac ttattagtca agtaggggaa
      601 taatttcagg gaactggtnt caaccttttt tttcagcttt ttccaaatca gagagagcag
      661 aaggtaatag aaggtgtaag aaaatgagat agatacatgc gtgggtcaat tgccttggca
      721 caccattaac ccccccgaag tgcaa
//
