LOCUS       sequence-22135-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Duennwald ML, Jagadish S, Muchowski PJ, Lindquist S
  TITLE     Flanking sequences profoundly alter polyglutamine toxicity in yeast.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50.
  PUBMED    16832050
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 18. 103(29):11045-50."
COMMENT     Sequence Label: GAL HA25Q+ProCFPp303
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     207..235
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     CDS             complement(545..571)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(604..627)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 gcatggcact cttgaaaaag tcatgccgtt tcatatgatc tgggtatctt gaaaagcatt
       61 gaacacccca agtcaaagta gtgacaagtg ttggccatgg aacaggtagt tttccagtag
      121 tgcaaataaa tttaagggta agttttccgt atgttgcatc accttcaccc tctccactga
      181 cagaaaattt gtgcccatta acatcaccat ctaattcaac aagaattggg acaactccag
      241 tgaaaagttc ttctccttta ctcagggatc caggtcggtg cagaggctcc tcagccacag
      301 ctgggcctgg aggaggtgga ggtggaggtg gaggaggttg tggctgaggc agcagaggct
      361 gtgcctgtgg aggaggttga ggaagttgag gaggtggggg tggaggggga ggtggtggcg
      421 gttgttgctg ttgctgctgt tgttgctgtt gctgctgttg ttgctgttgc tgctgttgtt
      481 gctgttgctg ctgttggaag cttttgaggg actcgaaggc cttcatcagc ttttccaggg
      541 tcgcagcgta gtctgggacg tcgtatgggt acatggctct agaatccggg gttttttctc
      601 cttgacgtta aagtatagag gtatattaac aattttttgt tgatactttt attacatttg
      661 aataagaagt aatacaaacc gaaaatgttg aaagtattag ttaaagtggt tatgcagttt
      721 ttgcatttat atatctgtta atagatcaaa aatcatcgct tcgctgatta attaccccag
      781 aaataaggct aaaaaactaa tcgcattatc atcctatggt tgttaatttg attcgttcat
      841 ttgaaggttt gtggggccag gttactgcca
//
