LOCUS       sequence-221350-         472 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of gRNA targeting TMTC4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Narimatsu Y, Joshi HJ, Zhang Y, Gomes C, Chen YH, Lorenzetti F, 
            Furukawa S, Schjoldager K, Hansen L, Clausen H, Bennett EP, Wandall 
            HH
  TITLE     A validated gRNA library for CRISPR/Cas9 targeting of the human 
            glycosyltransferase genome.
  JOURNAL   Glycobiology. 2018 Jan 5. pii: 4791732. doi: 10.1093/glycob/cwx101.
  PUBMED    29315387
REFERENCE   2  (bases 1 to 472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Glycobiology. 2018 Jan 5. pii: 4791732. doi: 
            10.1093/glycob/cwx101."
COMMENT     Sequence Label: gh163
FEATURES             Location/Qualifiers
     source          1..472
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     106..125
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        204..279
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa
       61 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt
      121 accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac
      181 accgctgcgt gcaaaacaca caagttttag agctagaaat agcaagttaa aataaggcta
      241 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttgtttt agagctagaa
      301 atagcaagtt aaaataaggc tagtccgttt ttagcgcgtg cgccaattct gcagacaaat
      361 ggctctagag ggccgcagga acccctagtg atggagttgg ccactcctct ctgcgcgctc
      421 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gc
//
