LOCUS       sequence-221355-       12610 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses gRNA in Aedes aegypti.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12610)
  AUTHORS   Li M, Yang T, Kandul NP, Bui M, Gamez S, Raban R, Bennett J, Sanchez
            C HM, Lanzaro GC, Schmidt H, Lee Y, Marshall JM, Akbari OS
  TITLE     Development of a confinable gene drive system in the human disease 
            vector Aedes aegypti.
  JOURNAL   Elife. 2020 Jan 21;9. pii: 51701. doi: 10.7554/eLife.51701.
  PUBMED    31960794
REFERENCE   2  (bases 1 to 12610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-01-21"; volume: "9. pii: 51701. doi"; pages: " 
            10.7554/eLife.51701"
COMMENT     Sequence Label: U6a
FEATURES             Location/Qualifiers
     source          1..12610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(175..194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(854..1645,1646..1714))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1477..1496
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1715..1819)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1887..1905
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1943..1965)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2065..2084
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2278..2300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2292..2309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2293..2309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   2998..3060
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     regulatory      7083..7092
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    8641..8762
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             join(10504..10506,10507..10509,10510..11184)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     polyA_signal    11298..11419
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   12087..12121
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(12303..12319)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12303..12319)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12316..12338)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12327..12343
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12351..12357,12358..12375,12376..12381))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12396..12417
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12534..12551)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaatat aacaaaattg
     2341 tgatcccaca aaatgaagtg gggcaaaatc aaataattaa ctagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcg tgactataac acaagttgat
     2461 ttaataattt tagccaacac gtcgggctgc gtgttttttg cgctctgtgt acacgttgat
     2521 taactggtcg attaaataat ttaatttttg gttcttcttt aaatctgtga tgaaattttt
     2581 taaaataact ttaaattctt cattggtaaa aaatgccacg ttttgcaact tgtgagggtc
     2641 taatatgagg tcaaactcag taggagtttt atccaaaaaa gaaaacatga ttacgtctgt
     2701 acacgaacgc gtattaacgc agagtgcaaa gtataagagg gttaaaaaat atattttacg
     2761 caccatatac gcatcgggtt gatatcgtta atatggatca atttgaacag ttgattaacg
     2821 tgtctctgct caagtctttg atcaaaacgc aaatcgacga aaatgtgtcg gacaatatca
     2881 agtcgatgag cgaaaaacta aaaaggctag aatacgacaa tctcacagac agcgttgaga
     2941 tatacggtat tcacgacagc aggctgaata ataaaaaaat tagaaactat tatttaaccc
     3001 tagaaagata atcatattgt gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg
     3061 tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat
     3121 tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat
     3181 ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttaaacat
     3241 tctctctttt acaaaaataa acttattttg tactttaaaa acagtcatgt tgtattataa
     3301 aataagtaat tagcttaact tatacataat agaaacaaat tatacttatt agtcagtcag
     3361 aaacaacttt ggcacatatc aatattatgc tctcgacaaa taactttttt gcattttttg
     3421 cacgatgcat ttgcctttcg ccttatttta gaggggcagt aagtacagta agtacgtttt
     3481 ttcattactg gctcttcagt actgtcatct gatgtaccag gcacttcatt tggcaaaata
     3541 ttagagatat tatcgcgcaa atatctcttc aaagtaggag cttctaaacg cttacgcata
     3601 aacgatgacg tcaggctcat gtaaaggttt ctcataaatt ttttgcgact ttgaaccttt
     3661 tctcccttgc tactgacatt atggctgtat ataataaaag aatttatgca ggcaatgttt
     3721 atcattccgt acaataatgc cataggccac ctattcgtct tcctactgca ggtcatcaca
     3781 gaacacattt ggtctagcgt gtccactccg cctttagttt gattataata cataaccatt
     3841 tgcggtttac cggtactttc gttgatagaa gcatcctcat cacaagatga taataagtat
     3901 accatcttag ctggcttcgg tttatatgag acgagagtaa ggggtccgtc aaaacaaaac
     3961 atcgatgttc ccactggcct ggagcgactg tttttcagta cttccggtat ctcgcgtttg
     4021 tttgatcgca cggttcccac aatggttaat tcgagctcgc ccggggtcct aggcctcacg
     4081 ccactcgtag aatgaacccg attgaatcat ttttaaaccg aaaaaaaaat cctgccggtg
     4141 ttctctgagt tttctgagga ttctgatcgg gatcccagag gaacctggga attgctattg
     4201 gattcctaac agactcttga gaattaccat cagagtttcg tacattttta acaagatttt
     4261 ttgtggaatc atcaccaaac catgaaaatt tctagcaggc ttttggggtt cctcagtggg
     4321 atcatgaaac tccgttacat cgtttttgct atgagattac agaaaaaata acttaagtaa
     4381 acagtttttc tgtaaaagat atttcactca cttagctttt gaacgcagtg gaattttaga
     4441 ccattgtttg cttgactttt tttttaatca taatttaaat gttagagtgt tagaaaccgt
     4501 gttcttataa acaatattct ccttttcttc ttccctgtct tgcgaaacaa acgatagaga
     4561 gaaacttatc agtgtacctg gcgtctaagc tgcgaatcaa ggaactacga ttttccagcc
     4621 acttcctatg taaatcaatc gaagtatcgt ttagtacatt acatttaata catttcttct
     4681 tcatctatgc atctttgact tttccaaaat gaatatccta tttcattata cattttttct
     4741 tttacttcca gcgctacagc accagcagtt atcaggatgt agatgaaggt atcaacagtt
     4801 cgttcggcag caatgacaaa tccacgctga ttcaggtttg gaaacccaaa agttatggcg
     4861 ccgtcaaggg acaaattcca cagcacgaac gactcacata cacatggaag gagatcgatg
     4921 tgtttggaga gacgccaggt gataccaaaa aggaacccct gtgcagtaga ttgtgctgtt
     4981 gtttctcccg ccagaagaaa gacttcaatc cccgcaaaca cctactgaag aatgtgaccg
     5041 ggatggctaa gagtggagag ttactggctg tgatgggtag ttctggcgcc ggtaaaacaa
     5101 ccttgctgaa cgctttgcta gcactttcca aaattcattt ggtaatttct acaagaattt
     5161 tttcaagaaa tttcttaaga atttttgaaa aaaaaaatgg taatttttac tgaattgata
     5221 aacgcaattg ataggtaaac cattgctaaa tatagtcatt caccaaacac actgcttttt
     5281 tctcgtctga aaatttttta gttgtttcgc aagtaaccca ttttatatcc aaaaattact
     5341 ttctgtacat ttttctcgct ctttcttagc gaatggatta ttgatttcta agcttgtcat
     5401 tgacttgact acttttattg aactgataat tctattaaca gaaattaata acaacagttt
     5461 gcttatgaaa taacatgata gccaagtcaa taacaggtcc agtttagact ttcgggtttt
     5521 ccatagtaaa tatactacaa ataatattaa tgttccatga aaaaaggagt aagagtctgg
     5581 taaccctagt gcacgcaaat atctcgcggg catatttggt tgctgaggta tatttatatt
     5641 tgaacgccat gagaaaaagc ggaagaaatt ggctcatggc cgattttaag gatatttaaa
     5701 aattgtacaa tgtacatata ataggccagg agaagtggat gaacttgtca ttcatttttc
     5761 tgtcaattct catacaaaat ctactttttc tttgacataa attcactcta ggtgaaccac
     5821 ttcccctggc ctattaaaca tccgttcctt caatgtgttc ttttttttaa gcgtgtgtta
     5881 aaagtttgct ctgctggtga attcacgctc tacccgttca ggcagcattc atcgaaaagc
     5941 cctatctgct cgcacacatt tacaaaatgc tgattgcgtt gtgtgctgaa tgggtcactc
     6001 gtccgtcact gcttgctgtg tacactgtac agttacgcag tctgtgcatc gctagaatca
     6061 tatttacgga agagtattat atatacccga tgcgttgctc ttcgattggt gcgatcttga
     6121 cccccgggtc ttagagctat gctggaaaca gcatagcaag ttaagataag gctagtccgt
     6181 tatcaacttg aaaaagtggc accgagtcgg tgcttttttt gtggttttac tattcgataa
     6241 ttgtgtctct tatcctcaga gtaatgtgaa aaaactacct agttaataaa attaataagg
     6301 ggagaaaggt ttgcaatttc ttacgtacat attttgttcc atatcatagt ggaatcacag
     6361 agacgatacc tccatcatgt ttaaggcagc atccatttat tacgtaacgc taaaattaga
     6421 aatttttgac ctccttcccc ccccccctcc gtaacgcttt ttgtatgaaa aatttctaat
     6481 ttttgtatga accgtaacga ttgagcctac tcccccctcc ccctagtgcg ttacgtaatt
     6541 tgtggatgcc gccttacgtg cagaaaatag ataacatagt ttcgagggcc tttaactttg
     6601 tttaaagtgc aacatttaaa tgctattaaa cttaagttta atatgtgtac agttgtatac
     6661 agttaacttt aagattatgt cactttaaaa caaaatctga tagcacacta tagagcaaac
     6721 ataaagtatt catatatagc ttcgtggtta cttgggaact gcgccatatc gtgtgcaaaa
     6781 tgaagaaata atacggagca gcatatactt tctgttagtt aattaattcg agctcgcccg
     6841 gggatctaat tcaattagag actaattcaa ttagagctaa ttcaattagg atccaagctt
     6901 atcgatttcg aaccctcgac cgccggagta taaatagagg cgcttcgtct acggagcgac
     6961 aattcaattc aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc aaataaacaa
     7021 gcgcagctga acaagctaaa caatcggggt accgctagag tcgacggtac gatccaccgg
     7081 tcgccaccat ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc
     7141 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     7201 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
     7261 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg
     7321 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     7381 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca
     7441 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc
     7501 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
     7561 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt
     7621 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca
     7681 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct
     7741 ccgagggccg ccaccacctg ttcctggggc atggcaccgg cagcaccggc agcggcagct
     7801 ccggcaccgc ctcctccgag gacaacaaca tggccgttat caaggaattt atgcgcttca
     7861 aagttaggat ggagggatcc atgaacggac atgagttcga gatcgaggga gagggcgagg
     7921 gacgcccgta tgaaggcaca caaacagcca aactcaaggt caccaagggc ggaccactgc
     7981 ccttcgcctg ggatatcctg agtccccagt ttatgtacgg cagcaaggcc tacgttaagc
     8041 accccgctga cataccggac tacaaaaagc tgtcctttcc ggaaggcttc aagtgggagc
     8101 gcgtgatgaa tttcgaagac ggaggactgg tcactgtgac ccaagatagc agtttgcagg
     8161 acggtacact gatctataag gttaaaatgc gcggcactaa ctttccgcca gatggcccag
     8221 tgatgcagaa gaagaccatg ggttgggagg catccaccga acgtctgtac cctcgagacg
     8281 gagtgctcaa gggcgagatc catcaggccc tcaaactgaa agatggtggt cactacctgg
     8341 tcgaatttaa gaccatttac atggccaaga agccggttca gctgcccgga tattattatg
     8401 tggatacgaa actggatata acttcgcata acgaagacta caccattgtc gagcagtatg
     8461 agcgcagcga aggccgacat cacctgttcc tctacggcat ggacgagctg tacaagtagg
     8521 cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta
     8581 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     8641 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8701 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8761 taaagcttgt ttaaactcaa gatcgcaccg acttcagtac gtgccctaaa cggaatccca
     8821 gtcaacgcgg aacagttgcg agcacgttgc gcctatgtgc aacaggacga tcttttcatt
     8881 cccgcgctta caactcgaga acacctcgta ttccacgcaa tgctgcgtat gggtaaagat
     8941 gtacccaaga gtgtcaagat gaatcgagtc aacgaggtgc ttcaggagct ttccttagcg
     9001 aaatgcgcag acacgatcat tggagcgcca ggtcgaatga aaggcctgtc cggaggtgaa
     9061 cggaaacggt tggcctttgc atcggaaacc ctgaccgatc ctcacctgtt gctttgtgat
     9121 gaacccactt ccgggttgga ttcgttcatg gctcacagtg ttctacaggt gctgaaggga
     9181 atggccctca aaggaaagac gattatactg accatacatc aaccttcgtc agaactgtat
     9241 tgcttgttcg acaaaattct cctagtggca gaaggacgcg tggctttcct tggatctccc
     9301 tatcaggctt cggaattctt ttctcagtga gtagacttgt gaaatcctga cgattttgct
     9361 ttactagcag ggcttgtggt gtcttgcctc ttaaaacacc gaacatgaaa cccaaaagcc
     9421 aaacgcaata ggaatcagac gatgagagtt cttcatagat ctgaccagac atgtctctaa
     9481 gaatatttct aagacttttt ttttggaaat tcatcgcgat cttgacatta cgcgtaatcc
     9541 gcgtaatcat cgtatttttc caaagttaca ttcagatatt acggcagaaa ttctttagga
     9601 gatttcatca ggagataaca tattggtgaa ttacggattc gttgtattta tcttcaagaa
     9661 tactacctga ttttagttta gcaattactt cagagatttc atttagaatt caagattcat
     9721 gcagaaatat tctttcattg atagaacttc cttctatgca gaacatctac cagaattcaa
     9781 ccagaaattt ctacttatat acctctaaca cggccaattg cggccagaca gtaaaataat
     9841 agtggcctta gtaactaaaa tgatcaaaat tgtgacccgg taaagtgatt taataaaaca
     9901 gtttttagtc caaacaatat taggggtcat ccaaaaacga cttcaatcat taggggcgcg
     9961 cccatactcg gtggcctccc caccaccaac ttttttgcac tgcaaaaaaa cacgcttttg
    10021 cacgcgggcc catacatagt acaaactcta cgtttcgtag actattttac ataaatagtc
    10081 tacaccgttg tatacgctcc aaatacacta ccacacattg aacctttttg cagtgcaaaa
    10141 aagtacgtgt cggcagtcac gtaggccggc cttatcgggt cgcgtcctgt cacgtacgaa
    10201 tcacattatc ggaccggacg agtgttgtct tatcgtgaca ggacgccagc ttcctgtgtt
    10261 gctaaccgca gccggacgca actccttatc ggaacaggac gcgcctccat atcagccgcg
    10321 cgttatctca tgcgcgtgac cggacacgag gcgcccgtcc cgcttatcgc gcctataaat
    10381 acagcccgca acgatctggt aaacacagtt gaacagcatc tgttacagcg acacaacatg
    10441 agccggtcca acaacgccaa cgcgcccacg ccatccaacc gccgccgcaa cctgtctctg
    10501 gtgatggtgc gctcctccaa gaacgtcatc aaggagttca tgcgcttcaa ggtgcgcatg
    10561 gagggcaccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
    10621 gagggccaca acaccgtgaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg
    10681 gacatcctgt ccccccagtt ccagtacggc tccaaggtgt acgtgaagca ccccgccgac
    10741 atccccgact acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
    10801 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggctgcttc
    10861 atctacaagg tgaagttcat cggcgtgaac ttcccctccg acggccccgt aatgcagaag
    10921 aagaccatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag
    10981 ggcgagatcc acaaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag
    11041 tccatctaca tggccaagaa gcccgtgcag ctgcccggct actactacgt ggactccaag
    11101 ctggacatca cctcccacaa cgaggactac accatcgtgg agcagtacga gcgcaccgag
    11161 ggccgccacc acctgttcct gtaggactct agatcataat cagccatacc acatttgtag
    11221 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
    11281 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    11341 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    11401 aactcatcaa tgtatcttaa agcttctagg ccggccgatc tcggatctga caatgttcag
    11461 tgcagagact cggctacgcc tcgtggactt tgaagttgac caacaatgtt tattcttacc
    11521 tctaatagtc ctctgtggca aggtcaagat tctgttagaa gccaatgaag aacctggttg
    11581 ttcaataaca ttttgttcgt ctaatatttc actaccgctt gacgttggct gcacttcatg
    11641 tacctcatct ataaacgctt cttctgtatc gctctggacg tcatcttcac ttacgtgatc
    11701 tgatatttca ctgtcagaat cctcaccaac aagctcgtca tcgctttgca gaagagcaga
    11761 gaggatatgc tcatcgtcta aagaactacc cattttatta tatattagtc acgatatcta
    11821 taacaagaaa atatatatat aataagttat cacgtaagta gaacatgaaa taacaatata
    11881 attatcgtat gagttaaatc ttaaaagtca cgtaaaagat aatcatgcgt cattttgact
    11941 cacgcggtcg ttatagttca aaatcagtga cacttaccgc attgacaagc acgcctcacg
    12001 ggagctccaa gcggcgactg agatgtccta aatgcacagc gacggattcg cgctatttag
    12061 aaagagagag caatatttca agaatgcatg cgtcaatttt acgcagacta tctttctagg
    12121 gttaaaaaag atttgcgctt tactcgacct aaactttaaa cacgtcatag aatcttcgtt
    12181 tgacaaaaac cacattgtgg ccaagctgtg tgacgcgacg cgcgctaaag aatggcaaac
    12241 caagtcgcgc gagcgtcgac tctagaggat ccccgggtac cgagctcgaa ttcgtaatca
    12301 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
    12361 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
    12421 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
    12481 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
    12541 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    12601 gtaatacggt
//
