LOCUS       sequence-22136-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Duennwald ML, Jagadish S, Muchowski PJ, Lindquist S
  TITLE     Flanking sequences profoundly alter polyglutamine toxicity in yeast.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50.
  PUBMED    16832050
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 18. 103(29):11045-50."
COMMENT     Sequence Label: GAL HA103Q+ProCFPp303
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     217..245
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     CDS             complement(771..797)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 taaccttcgg gcatggcact cttgaaaaag tcatgccgtt tcatatgatc tgggtatctt
       61 gaaaagcatt gaacacccca agtcaaagta gtgacaagtg ttggccatgg aacaggtagt
      121 tttccagtag tgcaaataaa tttaagggta agttttccgt atgttgcatc accttcaccc
      181 tctccactga cagaaaattt gtgcccatta acatcaccat ctaattcaac aagaattggg
      241 acaactccag tgaaaagttc ttctccttta ctcagggatc caggtcggtg cagaggctcc
      301 tcagccacag ctgggcctgg aggaggtgga ggtggaggtg gaggaggttg tggctgaggc
      361 agcagaggct gtgcctgtgg aggaggttga ggaagttgag gaggtggggg tggaggggga
      421 ggtggtggcg gttgttgctg ttgctgctgt tgttgctgtt gctgctgttg ttgctgttgc
      481 tgctgttgtt gctgttgctg ctgttgttgc tgttgctgct gttgttgctg ttgctgctgt
      541 tgttgctgtt gctgctgttg ttgctgttgc tgctgttgtt gctgttgctg ctgttgttgc
      601 tgttgctgct gttgttgctg ttgctgctgt tgttgctgtt gctgctgttg ttgctgttgc
      661 tgctgttgtt gctgttgctg ctgttgttgc tgttgctgct gttgttgctg ttgctgctgt
      721 tggaagcttt tgagggactc gaaggccttc atcagctttt ccagggtcgc agcgtagtct
      781 gggacgtcgt atgggtacat ggctctagaa tccggggttt tttctccttg acgttaaagt
      841 atagaggta
//
