LOCUS       sequence-221360-       12365 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses gRNA in Aedes aegypti.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12365)
  AUTHORS   Li M, Yang T, Kandul NP, Bui M, Gamez S, Raban R, Bennett J, Sanchez
            C HM, Lanzaro GC, Schmidt H, Lee Y, Marshall JM, Akbari OS
  TITLE     Development of a confinable gene drive system in the human disease 
            vector Aedes aegypti.
  JOURNAL   Elife. 2020 Jan 21;9. pii: 51701. doi: 10.7554/eLife.51701.
  PUBMED    31960794
REFERENCE   2  (bases 1 to 12365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-01-21"; volume: "9. pii: 51701. doi"; pages: " 
            10.7554/eLife.51701"
COMMENT     Sequence Label: U6c
FEATURES             Location/Qualifiers
     source          1..12365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(175..194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(854..1645,1646..1714))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1477..1496
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1715..1819)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1887..1905
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1943..1965)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2065..2084
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2278..2300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2292..2309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2293..2309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   2998..3060
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     regulatory      6838..6847
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    8396..8517
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             join(10259..10261,10262..10264,10265..10939)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     polyA_signal    11053..11174
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   11842..11876
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(12058..12074)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12058..12074)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12071..12093)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12082..12098
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12106..12112,12113..12130,12131..12136))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12151..12172
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12289..12306)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaatat aacaaaattg
     2341 tgatcccaca aaatgaagtg gggcaaaatc aaataattaa ctagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcg tgactataac acaagttgat
     2461 ttaataattt tagccaacac gtcgggctgc gtgttttttg cgctctgtgt acacgttgat
     2521 taactggtcg attaaataat ttaatttttg gttcttcttt aaatctgtga tgaaattttt
     2581 taaaataact ttaaattctt cattggtaaa aaatgccacg ttttgcaact tgtgagggtc
     2641 taatatgagg tcaaactcag taggagtttt atccaaaaaa gaaaacatga ttacgtctgt
     2701 acacgaacgc gtattaacgc agagtgcaaa gtataagagg gttaaaaaat atattttacg
     2761 caccatatac gcatcgggtt gatatcgtta atatggatca atttgaacag ttgattaacg
     2821 tgtctctgct caagtctttg atcaaaacgc aaatcgacga aaatgtgtcg gacaatatca
     2881 agtcgatgag cgaaaaacta aaaaggctag aatacgacaa tctcacagac agcgttgaga
     2941 tatacggtat tcacgacagc aggctgaata ataaaaaaat tagaaactat tatttaaccc
     3001 tagaaagata atcatattgt gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg
     3061 tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat
     3121 tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat
     3181 ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttaaacat
     3241 tctctctttt acaaaaataa acttattttg tactttaaaa acagtcatgt tgtattataa
     3301 aataagtaat tagcttaact tatacataat agaaacaaat tatacttatt agtcagtcag
     3361 aaacaacttt ggcacatatc aatattatgc tctcgacaaa taactttttt gcattttttg
     3421 cacgatgcat ttgcctttcg ccttatttta gaggggcagt aagtacagta agtacgtttt
     3481 ttcattactg gctcttcagt actgtcatct gatgtaccag gcacttcatt tggcaaaata
     3541 ttagagatat tatcgcgcaa atatctcttc aaagtaggag cttctaaacg cttacgcata
     3601 aacgatgacg tcaggctcat gtaaaggttt ctcataaatt ttttgcgact ttgaaccttt
     3661 tctcccttgc tactgacatt atggctgtat ataataaaag aatttatgca ggcaatgttt
     3721 atcattccgt acaataatgc cataggccac ctattcgtct tcctactgca ggtcatcaca
     3781 gaacacattt ggtctagcgt gtccactccg cctttagttt gattataata cataaccatt
     3841 tgcggtttac cggtactttc gttgatagaa gcatcctcat cacaagatga taataagtat
     3901 accatcttag ctggcttcgg tttatatgag acgagagtaa ggggtccgtc aaaacaaaac
     3961 atcgatgttc ccactggcct ggagcgactg tttttcagta cttccggtat ctcgcgtttg
     4021 tttgatcgca cggttcccac aatggttaat tcgagctcgc ccggggtcct aggcctcacg
     4081 ccactcgtag aatgaacccg attgaatcat ttttaaaccg aaaaaaaaat cctgccggtg
     4141 ttctctgagt tttctgagga ttctgatcgg gatcccagag gaacctggga attgctattg
     4201 gattcctaac agactcttga gaattaccat cagagtttcg tacattttta acaagatttt
     4261 ttgtggaatc atcaccaaac catgaaaatt tctagcaggc ttttggggtt cctcagtggg
     4321 atcatgaaac tccgttacat cgtttttgct atgagattac agaaaaaata acttaagtaa
     4381 acagtttttc tgtaaaagat atttcactca cttagctttt gaacgcagtg gaattttaga
     4441 ccattgtttg cttgactttt tttttaatca taatttaaat gttagagtgt tagaaaccgt
     4501 gttcttataa acaatattct ccttttcttc ttccctgtct tgcgaaacaa acgatagaga
     4561 gaaacttatc agtgtacctg gcgtctaagc tgcgaatcaa ggaactacga ttttccagcc
     4621 acttcctatg taaatcaatc gaagtatcgt ttagtacatt acatttaata catttcttct
     4681 tcatctatgc atctttgact tttccaaaat gaatatccta tttcattata cattttttct
     4741 tttacttcca gcgctacagc accagcagtt atcaggatgt agatgaaggt atcaacagtt
     4801 cgttcggcag caatgacaaa tccacgctga ttcaggtttg gaaacccaaa agttatggcg
     4861 ccgtcaaggg acaaattcca cagcacgaac gactcacata cacatggaag gagatcgatg
     4921 tgtttggaga gacgccaggt gataccaaaa aggaacccct gtgcagtaga ttgtgctgtt
     4981 gtttctcccg ccagaagaaa gacttcaatc cccgcaaaca cctactgaag aatgtgaccg
     5041 ggatggctaa gagtggagag ttactggctg tgatgggtag ttctggcgcc ggtaaaacaa
     5101 ccttgctgaa cgctttgcta gcatatgtct tgccgaagca cattaaccca agccatttat
     5161 atatcaagta caagttgatt tgattctagt gatggactga cacgaatcta tggcactgtt
     5221 gcagagctta caaacaggta cccggagtcg cttcaattat tcaatcatcc attcaaaaac
     5281 ttccatctct tccaattagc caatattcaa attggaatcc agccagccga cggaggcaga
     5341 tggtctacct ggcaactata gagtttccat gtttccagac tttccctccc ggtaaacgga
     5401 gacaaaacga cagacgtaag taggtacata tgcataccgc acggacaaat caaatttgtc
     5461 tggcagctcc aattagagtc gttaaaaatt taacgatgcg ctaaataact tcaagctatt
     5521 tgtctcgctg gattggcttc gagtggtaag atcctatcaa atgccgaaaa caaaaaactt
     5581 tcttcttaat tgttcgttct tcaacacctc tccatggtga taacggatac ggtttcattg
     5641 tcagcatcca tcctccgaaa aatacattac gccttgaaat atgcaatcgc aaacacggat
     5701 ctgtttggaa catttatttt actatgaaga gatgcgatag gtaatattta tttgagcgtt
     5761 taagatactc attgttctct caaagaatgt cattgaaagc caacgaggtc aaatcaaata
     5821 ttataataaa aaggtcaaag aggactaact taaagctctc tttatggata ggaaaaaata
     5881 ttttcgccca tcgctagaac ttttaccgtt tccattgagt atataactaa gatgaatgag
     5941 gctaattgat ggtgcgatct tgacccccgg gtcttagagc tatgctggaa acagcatagc
     6001 aagttaagat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
     6061 tttaagatgc ttcggcaatt aataataagg aatggtaaag cattcttcaa atgttactat
     6121 gttctcaaat tttcaagaat taggtattca agaaattctt tcaggcatat tccataatct
     6181 gcacaagcag ctactttaga aattattgcc gggcttaaat tatatgtaca acatttcctg
     6241 agtattttct gacaaaaatt ctcacaaggg aaggtcacga tggagttaca cggggttttt
     6301 aaccggacta tttttgttag attctacatg tcgaaatatg aaaaacccca aagcctttcg
     6361 ggtgatggac cagtggtgta gccaggaggg gctctgaaag gggtaatttt gtacgtatat
     6421 tatattatta agctaattgg aaatttgaca aaaatatttt tttagtattt attgtgatgg
     6481 tagagaacaa aattgacatt aatgtacgtt tgaaatgtat ttgccatgcc tttggcgtac
     6541 taacatggag tatggacacc aaaaccgatt tcgttaatta attcgagctc gcccggggat
     6601 ctaattcaat tagagactaa ttcaattaga gctaattcaa ttaggatcca agcttatcga
     6661 tttcgaaccc tcgaccgccg gagtataaat agaggcgctt cgtctacgga gcgacaattc
     6721 aattcaaaca agcaaagtga acacgtcgct aagcgaaagc taagcaaata aacaagcgca
     6781 gctgaacaag ctaaacaatc ggggtaccgc tagagtcgac ggtacgatcc accggtcgcc
     6841 accatggtga gcaagggcga ggaggtcatc aaagagttca tgcgcttcaa ggtgcgcatg
     6901 gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     6961 gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg
     7021 gacatcctgt ccccccagtt catgtacggc tccaaggcgt acgtgaagca ccccgccgac
     7081 atccccgatt acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     7141 ttcgaggacg gcggtctggt gaccgtgacc caggactcct ccctgcagga cggcacgctg
     7201 atctacaagg tgaagatgcg cggcaccaac ttcccccccg acggccccgt aatgcagaag
     7261 aagaccatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag
     7321 ggcgagatcc accaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag
     7381 accatctaca tggccaagaa gcccgtgcaa ctgcccggct actactacgt ggacaccaag
     7441 ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga gcgctccgag
     7501 ggccgccacc acctgttcct ggggcatggc accggcagca ccggcagcgg cagctccggc
     7561 accgcctcct ccgaggacaa caacatggcc gttatcaagg aatttatgcg cttcaaagtt
     7621 aggatggagg gatccatgaa cggacatgag ttcgagatcg agggagaggg cgagggacgc
     7681 ccgtatgaag gcacacaaac agccaaactc aaggtcacca agggcggacc actgcccttc
     7741 gcctgggata tcctgagtcc ccagtttatg tacggcagca aggcctacgt taagcacccc
     7801 gctgacatac cggactacaa aaagctgtcc tttccggaag gcttcaagtg ggagcgcgtg
     7861 atgaatttcg aagacggagg actggtcact gtgacccaag atagcagttt gcaggacggt
     7921 acactgatct ataaggttaa aatgcgcggc actaactttc cgccagatgg cccagtgatg
     7981 cagaagaaga ccatgggttg ggaggcatcc accgaacgtc tgtaccctcg agacggagtg
     8041 ctcaagggcg agatccatca ggccctcaaa ctgaaagatg gtggtcacta cctggtcgaa
     8101 tttaagacca tttacatggc caagaagccg gttcagctgc ccggatatta ttatgtggat
     8161 acgaaactgg atataacttc gcataacgaa gactacacca ttgtcgagca gtatgagcgc
     8221 agcgaaggcc gacatcacct gttcctctac ggcatggacg agctgtacaa gtaggcggcc
     8281 gcgactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     8341 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     8401 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     8461 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaaag
     8521 cttgtttaaa ctcaagatcg caccgacttc agtacgtgcc ctaaacggaa tcccagtcaa
     8581 cgcggaacag ttgcgagcac gttgcgccta tgtgcaacag gacgatcttt tcattcccgc
     8641 gcttacaact cgagaacacc tcgtattcca cgcaatgctg cgtatgggta aagatgtacc
     8701 caagagtgtc aagatgaatc gagtcaacga ggtgcttcag gagctttcct tagcgaaatg
     8761 cgcagacacg atcattggag cgccaggtcg aatgaaaggc ctgtccggag gtgaacggaa
     8821 acggttggcc tttgcatcgg aaaccctgac cgatcctcac ctgttgcttt gtgatgaacc
     8881 cacttccggg ttggattcgt tcatggctca cagtgttcta caggtgctga agggaatggc
     8941 cctcaaagga aagacgatta tactgaccat acatcaacct tcgtcagaac tgtattgctt
     9001 gttcgacaaa attctcctag tggcagaagg acgcgtggct ttccttggat ctccctatca
     9061 ggcttcggaa ttcttttctc agtgagtaga cttgtgaaat cctgacgatt ttgctttact
     9121 agcagggctt gtggtgtctt gcctcttaaa acaccgaaca tgaaacccaa aagccaaacg
     9181 caataggaat cagacgatga gagttcttca tagatctgac cagacatgtc tctaagaata
     9241 tttctaagac tttttttttg gaaattcatc gcgatcttga cattacgcgt aatccgcgta
     9301 atcatcgtat ttttccaaag ttacattcag atattacggc agaaattctt taggagattt
     9361 catcaggaga taacatattg gtgaattacg gattcgttgt atttatcttc aagaatacta
     9421 cctgatttta gtttagcaat tacttcagag atttcattta gaattcaaga ttcatgcaga
     9481 aatattcttt cattgataga acttccttct atgcagaaca tctaccagaa ttcaaccaga
     9541 aatttctact tatatacctc taacacggcc aattgcggcc agacagtaaa ataatagtgg
     9601 ccttagtaac taaaatgatc aaaattgtga cccggtaaag tgatttaata aaacagtttt
     9661 tagtccaaac aatattaggg gtcatccaaa aacgacttca atcattaggg gcgcgcccat
     9721 actcggtggc ctccccacca ccaacttttt tgcactgcaa aaaaacacgc ttttgcacgc
     9781 gggcccatac atagtacaaa ctctacgttt cgtagactat tttacataaa tagtctacac
     9841 cgttgtatac gctccaaata cactaccaca cattgaacct ttttgcagtg caaaaaagta
     9901 cgtgtcggca gtcacgtagg ccggccttat cgggtcgcgt cctgtcacgt acgaatcaca
     9961 ttatcggacc ggacgagtgt tgtcttatcg tgacaggacg ccagcttcct gtgttgctaa
    10021 ccgcagccgg acgcaactcc ttatcggaac aggacgcgcc tccatatcag ccgcgcgtta
    10081 tctcatgcgc gtgaccggac acgaggcgcc cgtcccgctt atcgcgccta taaatacagc
    10141 ccgcaacgat ctggtaaaca cagttgaaca gcatctgtta cagcgacaca acatgagccg
    10201 gtccaacaac gccaacgcgc ccacgccatc caaccgccgc cgcaacctgt ctctggtgat
    10261 ggtgcgctcc tccaagaacg tcatcaagga gttcatgcgc ttcaaggtgc gcatggaggg
    10321 caccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
    10381 ccacaacacc gtgaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat
    10441 cctgtccccc cagttccagt acggctccaa ggtgtacgtg aagcaccccg ccgacatccc
    10501 cgactacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
    10561 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggct gcttcatcta
    10621 caaggtgaag ttcatcggcg tgaacttccc ctccgacggc cccgtaatgc agaagaagac
    10681 catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga
    10741 gatccacaag gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagtccat
    10801 ctacatggcc aagaagcccg tgcagctgcc cggctactac tacgtggact ccaagctgga
    10861 catcacctcc cacaacgagg actacaccat cgtggagcag tacgagcgca ccgagggccg
    10921 ccaccacctg ttcctgtagg actctagatc ataatcagcc ataccacatt tgtagaggtt
    10981 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
    11041 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    11101 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    11161 atcaatgtat cttaaagctt ctaggccggc cgatctcgga tctgacaatg ttcagtgcag
    11221 agactcggct acgcctcgtg gactttgaag ttgaccaaca atgtttattc ttacctctaa
    11281 tagtcctctg tggcaaggtc aagattctgt tagaagccaa tgaagaacct ggttgttcaa
    11341 taacattttg ttcgtctaat atttcactac cgcttgacgt tggctgcact tcatgtacct
    11401 catctataaa cgcttcttct gtatcgctct ggacgtcatc ttcacttacg tgatctgata
    11461 tttcactgtc agaatcctca ccaacaagct cgtcatcgct ttgcagaaga gcagagagga
    11521 tatgctcatc gtctaaagaa ctacccattt tattatatat tagtcacgat atctataaca
    11581 agaaaatata tatataataa gttatcacgt aagtagaaca tgaaataaca atataattat
    11641 cgtatgagtt aaatcttaaa agtcacgtaa aagataatca tgcgtcattt tgactcacgc
    11701 ggtcgttata gttcaaaatc agtgacactt accgcattga caagcacgcc tcacgggagc
    11761 tccaagcggc gactgagatg tcctaaatgc acagcgacgg attcgcgcta tttagaaaga
    11821 gagagcaata tttcaagaat gcatgcgtca attttacgca gactatcttt ctagggttaa
    11881 aaaagatttg cgctttactc gacctaaact ttaaacacgt catagaatct tcgtttgaca
    11941 aaaaccacat tgtggccaag ctgtgtgacg cgacgcgcgc taaagaatgg caaaccaagt
    12001 cgcgcgagcg tcgactctag aggatccccg ggtaccgagc tcgaattcgt aatcatggtc
    12061 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
    12121 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
    12181 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
    12241 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
    12301 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    12361 acggt
//
