LOCUS       sequence-221363-       12423 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses gRNA in Aedes aegypti.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12423)
  AUTHORS   Li M, Yang T, Kandul NP, Bui M, Gamez S, Raban R, Bennett J, Sanchez
            C HM, Lanzaro GC, Schmidt H, Lee Y, Marshall JM, Akbari OS
  TITLE     Development of a confinable gene drive system in the human disease 
            vector Aedes aegypti.
  JOURNAL   Elife. 2020 Jan 21;9. pii: 51701. doi: 10.7554/eLife.51701.
  PUBMED    31960794
REFERENCE   2  (bases 1 to 12423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-01-21"; volume: "9. pii: 51701. doi"; pages: " 
            10.7554/eLife.51701"
COMMENT     Sequence Label: U6d
FEATURES             Location/Qualifiers
     source          1..12423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(175..194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(854..1645,1646..1714))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1477..1496
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1715..1819)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1887..1905
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1943..1965)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2065..2084
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2278..2300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2292..2309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2293..2309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   2998..3060
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     regulatory      6896..6905
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    8454..8575
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             join(10317..10319,10320..10322,10323..10997)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     polyA_signal    11111..11232
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   11900..11934
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(12116..12132)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12116..12132)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12129..12151)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12140..12156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12164..12170,12171..12188,12189..12194))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12209..12230
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12347..12364)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaatat aacaaaattg
     2341 tgatcccaca aaatgaagtg gggcaaaatc aaataattaa ctagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcg tgactataac acaagttgat
     2461 ttaataattt tagccaacac gtcgggctgc gtgttttttg cgctctgtgt acacgttgat
     2521 taactggtcg attaaataat ttaatttttg gttcttcttt aaatctgtga tgaaattttt
     2581 taaaataact ttaaattctt cattggtaaa aaatgccacg ttttgcaact tgtgagggtc
     2641 taatatgagg tcaaactcag taggagtttt atccaaaaaa gaaaacatga ttacgtctgt
     2701 acacgaacgc gtattaacgc agagtgcaaa gtataagagg gttaaaaaat atattttacg
     2761 caccatatac gcatcgggtt gatatcgtta atatggatca atttgaacag ttgattaacg
     2821 tgtctctgct caagtctttg atcaaaacgc aaatcgacga aaatgtgtcg gacaatatca
     2881 agtcgatgag cgaaaaacta aaaaggctag aatacgacaa tctcacagac agcgttgaga
     2941 tatacggtat tcacgacagc aggctgaata ataaaaaaat tagaaactat tatttaaccc
     3001 tagaaagata atcatattgt gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg
     3061 tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat
     3121 tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat
     3181 ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttaaacat
     3241 tctctctttt acaaaaataa acttattttg tactttaaaa acagtcatgt tgtattataa
     3301 aataagtaat tagcttaact tatacataat agaaacaaat tatacttatt agtcagtcag
     3361 aaacaacttt ggcacatatc aatattatgc tctcgacaaa taactttttt gcattttttg
     3421 cacgatgcat ttgcctttcg ccttatttta gaggggcagt aagtacagta agtacgtttt
     3481 ttcattactg gctcttcagt actgtcatct gatgtaccag gcacttcatt tggcaaaata
     3541 ttagagatat tatcgcgcaa atatctcttc aaagtaggag cttctaaacg cttacgcata
     3601 aacgatgacg tcaggctcat gtaaaggttt ctcataaatt ttttgcgact ttgaaccttt
     3661 tctcccttgc tactgacatt atggctgtat ataataaaag aatttatgca ggcaatgttt
     3721 atcattccgt acaataatgc cataggccac ctattcgtct tcctactgca ggtcatcaca
     3781 gaacacattt ggtctagcgt gtccactccg cctttagttt gattataata cataaccatt
     3841 tgcggtttac cggtactttc gttgatagaa gcatcctcat cacaagatga taataagtat
     3901 accatcttag ctggcttcgg tttatatgag acgagagtaa ggggtccgtc aaaacaaaac
     3961 atcgatgttc ccactggcct ggagcgactg tttttcagta cttccggtat ctcgcgtttg
     4021 tttgatcgca cggttcccac aatggttaat tcgagctcgc ccggggtcct aggcctcacg
     4081 ccactcgtag aatgaacccg attgaatcat ttttaaaccg aaaaaaaaat cctgccggtg
     4141 ttctctgagt tttctgagga ttctgatcgg gatcccagag gaacctggga attgctattg
     4201 gattcctaac agactcttga gaattaccat cagagtttcg tacattttta acaagatttt
     4261 ttgtggaatc atcaccaaac catgaaaatt tctagcaggc ttttggggtt cctcagtggg
     4321 atcatgaaac tccgttacat cgtttttgct atgagattac agaaaaaata acttaagtaa
     4381 acagtttttc tgtaaaagat atttcactca cttagctttt gaacgcagtg gaattttaga
     4441 ccattgtttg cttgactttt tttttaatca taatttaaat gttagagtgt tagaaaccgt
     4501 gttcttataa acaatattct ccttttcttc ttccctgtct tgcgaaacaa acgatagaga
     4561 gaaacttatc agtgtacctg gcgtctaagc tgcgaatcaa ggaactacga ttttccagcc
     4621 acttcctatg taaatcaatc gaagtatcgt ttagtacatt acatttaata catttcttct
     4681 tcatctatgc atctttgact tttccaaaat gaatatccta tttcattata cattttttct
     4741 tttacttcca gcgctacagc accagcagtt atcaggatgt agatgaaggt atcaacagtt
     4801 cgttcggcag caatgacaaa tccacgctga ttcaggtttg gaaacccaaa agttatggcg
     4861 ccgtcaaggg acaaattcca cagcacgaac gactcacata cacatggaag gagatcgatg
     4921 tgtttggaga gacgccaggt gataccaaaa aggaacccct gtgcagtaga ttgtgctgtt
     4981 gtttctcccg ccagaagaaa gacttcaatc cccgcaaaca cctactgaag aatgtgaccg
     5041 ggatggctaa gagtggagag ttactggctg tgatgggtag ttctggcgcc ggtaaaacaa
     5101 ccttgctgaa cgctttgcta gctcattcat cttagttata tactcaatgg aaacggtaaa
     5161 agttctagcg atgggcgaaa atattttttc ctatccataa agagagcttt aagttagtcc
     5221 tctttgacct ttttattata atatttgatt tgacctcgtt ggctttcaat gacattcttt
     5281 gagagaacaa tgagtatctt aaacgctcaa ataaatatta cctatcgcat ctcttcatag
     5341 taaaataaat gttccaaaca gatccgtgtt tgcgattgca tatttcaagg cgtaatgtat
     5401 ttttcggagg atggatgctg acaatgaaac cgtatccgtt atcaccatgg agaggtgttg
     5461 aagaacgaac aattaagaag aaagtttttt gttttcggca tttgatagga tcttaccact
     5521 cgaagccaat ccagcgagac aaatagcttg aagttattta gcgcatcgtt aaatttttaa
     5581 cgactctaat tggagctgcc agacaaattt gatttgtccg tgcggtatgc atatgtacct
     5641 acttacgtct gtcgttttgt ctccgtttac cgggagggaa agtctggaaa catggaaact
     5701 ctatagttgc caggtagacc atctgcctcc gtcggctggc tggattccaa tttgaatatt
     5761 ggctaattgg aagagatgga agtttttgaa tggatgattg aataattgaa gcgactccgg
     5821 gtacctgttt gtaagctctg caacagtgcc atagattcgt gtcagtccat cactagaatc
     5881 aaatcaactt gtacttgata tataaatggc ttgggttaat ggtgcgatct tgacccccgg
     5941 gtcttagagc tatgctggaa acagcatagc aagttaagat aaggctagtc cgttatcaac
     6001 ttgaaaaagt ggcaccgagt cggtgctttt tttacaaatc tattatgcat gaggtactat
     6061 gttggcagag taatgaatgc ctaaaaaaat atatgaacat taatatggcc ttaaaaagaa
     6121 ctgtctatat cttcaaaata ctgcaaccat caaacccaaa catagaatga acctatttaa
     6181 gtaaagtagc tatcatttga aacctacgaa atcaatgaat ttgttgaatt attatttgac
     6241 ctataagatt ctgtacggaa aattcttaaa tatgtttaaa catcttattt taaattataa
     6301 tgtgttcatg tcttcttctc tcttttcagg tacttacaac agcgttatga aatcctgttc
     6361 tgatacggta tgttttggtt tgttcgttct aaagtgacag ttttctttta aatgctttca
     6421 tgaactaatt taaagcgatg gagcttagaa aacaatttcc gaatctataa agcattttgt
     6481 ttgcagaagt tgacacaagt agaatctgtg ttttgactgt ataaaagttt tcccctcgtt
     6541 tttccttatt attatttcct ccatttcctt ggccgtcaca ctgcatttcc ggtgcggcaa
     6601 agtcgggcga cgacgaccac cacaggtgtt cttaattaat tcgagctcgc ccggggatct
     6661 aattcaatta gagactaatt caattagagc taattcaatt aggatccaag cttatcgatt
     6721 tcgaaccctc gaccgccgga gtataaatag aggcgcttcg tctacggagc gacaattcaa
     6781 ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc
     6841 tgaacaagct aaacaatcgg ggtaccgcta gagtcgacgg tacgatccac cggtcgccac
     6901 catggtgagc aagggcgagg aggtcatcaa agagttcatg cgcttcaagg tgcgcatgga
     6961 gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     7021 gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga
     7081 catcctgtcc ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat
     7141 ccccgattac aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     7201 cgaggacggc ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat
     7261 ctacaaggtg aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa
     7321 gaccatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg
     7381 cgagatccac caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac
     7441 catctacatg gccaagaagc ccgtgcaact gcccggctac tactacgtgg acaccaagct
     7501 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg
     7561 ccgccaccac ctgttcctgg ggcatggcac cggcagcacc ggcagcggca gctccggcac
     7621 cgcctcctcc gaggacaaca acatggccgt tatcaaggaa tttatgcgct tcaaagttag
     7681 gatggaggga tccatgaacg gacatgagtt cgagatcgag ggagagggcg agggacgccc
     7741 gtatgaaggc acacaaacag ccaaactcaa ggtcaccaag ggcggaccac tgcccttcgc
     7801 ctgggatatc ctgagtcccc agtttatgta cggcagcaag gcctacgtta agcaccccgc
     7861 tgacataccg gactacaaaa agctgtcctt tccggaaggc ttcaagtggg agcgcgtgat
     7921 gaatttcgaa gacggaggac tggtcactgt gacccaagat agcagtttgc aggacggtac
     7981 actgatctat aaggttaaaa tgcgcggcac taactttccg ccagatggcc cagtgatgca
     8041 gaagaagacc atgggttggg aggcatccac cgaacgtctg taccctcgag acggagtgct
     8101 caagggcgag atccatcagg ccctcaaact gaaagatggt ggtcactacc tggtcgaatt
     8161 taagaccatt tacatggcca agaagccggt tcagctgccc ggatattatt atgtggatac
     8221 gaaactggat ataacttcgc ataacgaaga ctacaccatt gtcgagcagt atgagcgcag
     8281 cgaaggccga catcacctgt tcctctacgg catggacgag ctgtacaagt aggcggccgc
     8341 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     8401 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     8461 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     8521 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaagct
     8581 tgtttaaact caagatcgca ccgacttcag tacgtgccct aaacggaatc ccagtcaacg
     8641 cggaacagtt gcgagcacgt tgcgcctatg tgcaacagga cgatcttttc attcccgcgc
     8701 ttacaactcg agaacacctc gtattccacg caatgctgcg tatgggtaaa gatgtaccca
     8761 agagtgtcaa gatgaatcga gtcaacgagg tgcttcagga gctttcctta gcgaaatgcg
     8821 cagacacgat cattggagcg ccaggtcgaa tgaaaggcct gtccggaggt gaacggaaac
     8881 ggttggcctt tgcatcggaa accctgaccg atcctcacct gttgctttgt gatgaaccca
     8941 cttccgggtt ggattcgttc atggctcaca gtgttctaca ggtgctgaag ggaatggccc
     9001 tcaaaggaaa gacgattata ctgaccatac atcaaccttc gtcagaactg tattgcttgt
     9061 tcgacaaaat tctcctagtg gcagaaggac gcgtggcttt ccttggatct ccctatcagg
     9121 cttcggaatt cttttctcag tgagtagact tgtgaaatcc tgacgatttt gctttactag
     9181 cagggcttgt ggtgtcttgc ctcttaaaac accgaacatg aaacccaaaa gccaaacgca
     9241 ataggaatca gacgatgaga gttcttcata gatctgacca gacatgtctc taagaatatt
     9301 tctaagactt tttttttgga aattcatcgc gatcttgaca ttacgcgtaa tccgcgtaat
     9361 catcgtattt ttccaaagtt acattcagat attacggcag aaattcttta ggagatttca
     9421 tcaggagata acatattggt gaattacgga ttcgttgtat ttatcttcaa gaatactacc
     9481 tgattttagt ttagcaatta cttcagagat ttcatttaga attcaagatt catgcagaaa
     9541 tattctttca ttgatagaac ttccttctat gcagaacatc taccagaatt caaccagaaa
     9601 tttctactta tatacctcta acacggccaa ttgcggccag acagtaaaat aatagtggcc
     9661 ttagtaacta aaatgatcaa aattgtgacc cggtaaagtg atttaataaa acagttttta
     9721 gtccaaacaa tattaggggt catccaaaaa cgacttcaat cattaggggc gcgcccatac
     9781 tcggtggcct ccccaccacc aacttttttg cactgcaaaa aaacacgctt ttgcacgcgg
     9841 gcccatacat agtacaaact ctacgtttcg tagactattt tacataaata gtctacaccg
     9901 ttgtatacgc tccaaataca ctaccacaca ttgaaccttt ttgcagtgca aaaaagtacg
     9961 tgtcggcagt cacgtaggcc ggccttatcg ggtcgcgtcc tgtcacgtac gaatcacatt
    10021 atcggaccgg acgagtgttg tcttatcgtg acaggacgcc agcttcctgt gttgctaacc
    10081 gcagccggac gcaactcctt atcggaacag gacgcgcctc catatcagcc gcgcgttatc
    10141 tcatgcgcgt gaccggacac gaggcgcccg tcccgcttat cgcgcctata aatacagccc
    10201 gcaacgatct ggtaaacaca gttgaacagc atctgttaca gcgacacaac atgagccggt
    10261 ccaacaacgc caacgcgccc acgccatcca accgccgccg caacctgtct ctggtgatgg
    10321 tgcgctcctc caagaacgtc atcaaggagt tcatgcgctt caaggtgcgc atggagggca
    10381 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggcc
    10441 acaacaccgt gaagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc
    10501 tgtcccccca gttccagtac ggctccaagg tgtacgtgaa gcaccccgcc gacatccccg
    10561 actacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
    10621 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggctgc ttcatctaca
    10681 aggtgaagtt catcggcgtg aacttcccct ccgacggccc cgtaatgcag aagaagacca
    10741 tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga
    10801 tccacaaggc cctgaagctg aaggacggcg gccactacct ggtggagttc aagtccatct
    10861 acatggccaa gaagcccgtg cagctgcccg gctactacta cgtggactcc aagctggaca
    10921 tcacctccca caacgaggac tacaccatcg tggagcagta cgagcgcacc gagggccgcc
    10981 accacctgtt cctgtaggac tctagatcat aatcagccat accacatttg tagaggtttt
    11041 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
    11101 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    11161 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    11221 caatgtatct taaagcttct aggccggccg atctcggatc tgacaatgtt cagtgcagag
    11281 actcggctac gcctcgtgga ctttgaagtt gaccaacaat gtttattctt acctctaata
    11341 gtcctctgtg gcaaggtcaa gattctgtta gaagccaatg aagaacctgg ttgttcaata
    11401 acattttgtt cgtctaatat ttcactaccg cttgacgttg gctgcacttc atgtacctca
    11461 tctataaacg cttcttctgt atcgctctgg acgtcatctt cacttacgtg atctgatatt
    11521 tcactgtcag aatcctcacc aacaagctcg tcatcgcttt gcagaagagc agagaggata
    11581 tgctcatcgt ctaaagaact acccatttta ttatatatta gtcacgatat ctataacaag
    11641 aaaatatata tataataagt tatcacgtaa gtagaacatg aaataacaat ataattatcg
    11701 tatgagttaa atcttaaaag tcacgtaaaa gataatcatg cgtcattttg actcacgcgg
    11761 tcgttatagt tcaaaatcag tgacacttac cgcattgaca agcacgcctc acgggagctc
    11821 caagcggcga ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt tagaaagaga
    11881 gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga ctatctttct agggttaaaa
    11941 aagatttgcg ctttactcga cctaaacttt aaacacgtca tagaatcttc gtttgacaaa
    12001 aaccacattg tggccaagct gtgtgacgcg acgcgcgcta aagaatggca aaccaagtcg
    12061 cgcgagcgtc gactctagag gatccccggg taccgagctc gaattcgtaa tcatggtcat
    12121 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
    12181 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
    12241 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
    12301 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
    12361 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    12421 ggt
//
