LOCUS       sequence-22137-e         667 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Duennwald ML, Jagadish S, Muchowski PJ, Lindquist S
  TITLE     Flanking sequences profoundly alter polyglutamine toxicity in yeast.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50.
  PUBMED    16832050
REFERENCE   2  (bases 1 to 667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 18. 103(29):11045-50."
COMMENT     Sequence Label: GAL FLAG25Q+ProGFPp303
FEATURES             Location/Qualifiers
     source          1..667
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..23
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     primer_bind     complement(337..365)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctacaaggga cgacgatgac aagctgggcg accctggaaa agctgatgaa ggccttcgag
       61 tccctcaaaa gcttccaaca gcagcaacag caacaacagc agcaacagca acaacagcag
      121 caacagcaac aacagcagca acagcaacaa ccgccaccac ctccccctcc acccccacct
      181 cctcaacttc ctcaacctcc tccacaggca cagcctctgc tgcctcagcc acaacctcct
      241 ccacctccac ctccacctcc tccaggccca gctgtggctg aggagcctct gcaccgacct
      301 ggatccctga gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
      361 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
      421 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca
      481 acacttgtca ctactttgac ttggggtgtt caatgctttt caagataccc agatcatatg
      541 aaacggcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata
      601 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc
      661 cttgtta
//
