LOCUS       sequence-221384-        8553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of exogenous LAP tagged MPS1 Kinase-dead.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8553)
  AUTHORS   Nijenhuis W, von Castelmur E, Littler D, De Marco V, Tromer E, 
            Vleugel M, van Osch MH, Snel B, Perrakis A, Kops GJ
  TITLE     A TPR domain-containing N-terminal module of MPS1 is required for 
            its kinetochore localization by Aurora B.
  JOURNAL   J Cell Biol. 2013 Apr 15;201(2):217-31. doi: 10.1083/jcb.201210033. 
            Epub 2013 Apr 8.
  PUBMED    23569217
REFERENCE   2  (bases 1 to 8553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201210033"; journalName: "J Cell Biol"; date: 
            "2013-04-15- 15"; volume: "201"; issue: "2"; pages: "217-31"
COMMENT     Sequence Label: pCDNA5-LAP-MPS1-KD
FEATURES             Location/Qualifiers
     source          1..8553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        142..521
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        522..725
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     676..696
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    727..745
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    748..766
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     776..800
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      925..934
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(931..933,934..936,937..1647)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(976..997)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1237..1256)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1584..1605
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1693..1713
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1726..1770
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(4412..4429)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4418..4642
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4775..4794)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4926..4973
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4981..6003
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4981..6003
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6133..6254
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6170..6189)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6224..6243
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6303..6319)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6303..6319)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6316..6338)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6327..6343
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6351..6357,6358..6375,6376..6381))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6396..6417
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6534..6551)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6705..7293)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6785..6804)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7464..8255,8256..8324))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8087..8106
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8325..8429)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8504..8523)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
       61 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      121 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      181 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      241 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      301 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      361 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      421 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      481 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      541 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      601 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      661 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      721 gagctctccc tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc
      781 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
      841 gacaccggga ccgatccagc ctccggactc tagcgtttaa acttaagctt ggtaccgagc
      901 tcggatccgc tagcgctacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
      961 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     1021 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     1081 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcag
     1141 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     1201 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     1261 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     1321 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     1381 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     1441 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     1501 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa
     1561 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     1621 actctcggca tggacgagct gtacaagtcc ggaggggatc ggtggagctc caccggtggc
     1681 ggccgctcta gagagaatct ttattttcag ggcgccgcca aattcaaaga aaccgctgct
     1741 gctaaattcg aacgccagca catggacagc ggaggtggag gtactagtct cgagatggaa
     1801 tccgaggatt taagtggcag agaattgaca attgattcca taatgaacaa agtgagagac
     1861 attaaaaata agtttaaaaa tgaagacctt actgatgaac taagcttgaa taaaatttct
     1921 gctgatacta cagataactc gggaactgtt aaccaaatta tgatgatggc aaacaaccca
     1981 gaggactggt tgagtttgtt gctcaaacta gagaaaaaca gtgttccgct aagtgatgct
     2041 cttttaaata aattgattgg tcgttacagt caagcaattg aagcgcttcc cccagataaa
     2101 tatggccaaa atgagagttt tgctagaatt caagtgagat ttgctgaatt aaaagctatt
     2161 caagagccag atgatgcacg tgactacttt caaatggcca gagcaaactg caagaaattt
     2221 gcttttgttc atatatcttt tgcacaattt gaactgtcac aaggtaatgt caaaaaaagt
     2281 aaacaacttc ttcaaaaagc tgtagaacgt ggagcagtac cactagaaat gctggaaatt
     2341 gccctgcgga atttaaacct ccaaaaaaag cagctgcttt cagaggagga aaagaagaat
     2401 ttatcagcat ctacggtatt aactgcccaa gaatcatttt ccggttcact tgggcattta
     2461 cagaatagga acaacagttg tgattccaga ggacagacta ctaaagccag gtttttatat
     2521 ggagagaaca tgccaccaca agatgcagaa ataggttacc ggaattcatt gagacaaact
     2581 aacaaaacta aacagtcatg cccatttgga agagtcccag ttaaccttct aaatagccca
     2641 gattgtgatg tgaagacgga cgattcagtt gtaccttgtt ttatgaaaag acaaacctct
     2701 agatcagaat gccgagattt ggttgtgcct ggatctaaac caagtggaaa tgattcctgt
     2761 gaattaagaa atttaaagtc tgttcaaaat agtcatttca aggaacctct ggtgtcagat
     2821 gaaaagagtt ctgaacttat tattactgat tcaataaccc tgaagaataa aacggaatca
     2881 agtcttctag ctaaattaga agaaactaaa gagtatcaag aaccagaggt tccagagagt
     2941 aaccagaaac agtggcaatc taagagaaag tcagagtgta ttaaccagaa tcctgctgca
     3001 tcttcaaatc actggcagat tccggagtta gcccgaaaag ttaatacaga gcagaaacat
     3061 accacttttg agcaacctgt cttttcagtt tcaaaacagt caccaccaat atcaacatct
     3121 aaatggtttg acccaaaatc tatttgtaag acaccaagca gcaatacctt ggatgattac
     3181 atgagctgtt ttagaactcc agttgtaaag aatgactttc cacctgcttg tcagttgtca
     3241 acaccttatg gccaacctgc ctgtttccag cagcaacagc atcaaatact tgccactcca
     3301 cttcaaaatt tacaggtttt agcatcttct tcagcaaatg aatgcatttc ggttaaagga
     3361 agaatttatt ccatattaaa gcagatagga agtggaggtt caagcaaggt atttcaggtg
     3421 ttaaatgaaa agaaacagat atatgctata aaatatgtga acttagaaga agcagataac
     3481 caaactcttg atagttaccg gaacgaaata gcttatttga ataaactaca acaacacagt
     3541 gataagatca tccgacttta tgattatgaa atcacggacc agtacatcta catggtaatg
     3601 gagtgtggaa atattgatct taatagttgg cttaaaaaga aaaaatccat tgatccatgg
     3661 gaacgcaaga gttactggaa aaatatgtta gaggcagttc acacaatcca tcaacatggc
     3721 attgttcaca gtgatcttaa accagctaac tttctgatag ttgatggaat gctaaagcta
     3781 attgcttttg ggattgcaaa ccaaatgcaa ccagatacaa caagtgttgt taaagattct
     3841 caggttggca cagttaatta tatgccacca gaagcaatca aagatatgtc ttcctccaga
     3901 gagaatggga aatctaagtc aaagataagc cccaaaagtg atgtttggtc cttaggatgt
     3961 attttgtact atatgactta cgggaaaaca ccatttcagc agataattaa tcagatttct
     4021 aaattacatg ccataattga tcctaatcat gaaattgaat ttcccgatat tccagagaaa
     4081 gatcttcaag atgtgttaaa gtgttgttta aaaagggacc caaaacagag gatatccatt
     4141 cctgagctcc tggctcatcc atatgttcaa attcaaactc atccagttaa ccaaatggcc
     4201 aagggaacca ctgaagaaat gaaatatgtt ctgggccaac ttgttggtct gaattctcct
     4261 aactccattt tgaaagctgc taaaacttta tatgaacact atagtggtgg tgaaagtcat
     4321 aattcttcat cctccaagac ttttgaaaaa aaaaggggaa aaaaatgagt cgagcatgca
     4381 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     4441 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     4501 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     4561 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     4621 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     4681 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4741 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4801 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4861 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     4921 acctagaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttccttggcc
     4981 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     5041 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     5101 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     5161 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     5221 gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
     5281 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
     5341 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     5401 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     5461 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     5521 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     5581 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     5641 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     5701 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
     5761 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     5821 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     5881 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     5941 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa
     6001 tagcacgtac tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     6061 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     6121 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6181 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6241 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     6301 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6361 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6421 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     6481 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     6541 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6601 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6661 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6721 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6781 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6841 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6901 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6961 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7021 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7081 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7141 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7201 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7261 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7321 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7381 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7441 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7501 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7561 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7621 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7681 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7741 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7801 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7861 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7921 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7981 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8041 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8101 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8161 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8221 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8281 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8341 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8401 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     8461 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
     8521 ccgcatagtt aagccagtat ctgctccctg ctt
//
