LOCUS       sequence-22142-e         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Duennwald ML, Jagadish S, Muchowski PJ, Lindquist S
  TITLE     Flanking sequences profoundly alter polyglutamine toxicity in yeast.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50.
  PUBMED    16832050
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 18. 103(29):11045-50."
COMMENT     Sequence Label: GAL HA25QDProCFPp303
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..39
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(211..239)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tctagagcca tgtacccata cgacgtccca gactacgctg cgaccctgga aaagctgatg
       61 aaggccttcg agtccctcaa aagcttccaa cagcagcaac agcaacaaca gcagcaacag
      121 caacaacagc agcaacagca acaacagcag caacagcaac aactgcagcc cgggggatcc
      181 ctgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      241 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      301 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      361 gtcactactt tgacttgggg tgttcaatgc ttttcaagat acccagatca tatgaaacgg
      421 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      481 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      541 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      601 ttggaataca actatatttc acacaatgta tacatcactg cagacaaaca aaagaatgga
      661 atcaaagctc atttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      721 cattatcaac aaaatactcc aattggcgat gg
//
