LOCUS       sequence-221425-        7982 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes human DPP6 variant 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7982)
  AUTHORS   Lainez S, Doray A, Hancox JC, Cannell MB
  TITLE     Regulation of Kv4.3 and hERG potassium channels by KChIP2 isoforms 
            and DPP6 and response to the dual K(+) channel activator NS3623.
  JOURNAL   Biochem Pharmacol. 2018 Apr;150:120-130. doi: 
            10.1016/j.bcp.2018.01.036. Epub 2018 Jan 31.
  PUBMED    29378180
REFERENCE   2  (bases 1 to 7982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Pharmacol."; date: "2018-04"; volume: "150:120-130. doi"; pages: " 
            10.1016/j.bcp.2018.01.036. Epub 2018 Jan 31"
COMMENT     Sequence Label: pcDNA3.1+DPP6
FEATURES             Location/Qualifiers
     source          1..7982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      935..944
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3576..3593)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3582..3806
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3852..4280
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3939..3958)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4149..4170
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4289..4309)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4294..4623
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4474..4609
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4536..4555
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4690..5484
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4744..4763)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5354..5373
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5658..5779
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5695..5714)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5749..5768
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5828..5844)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5828..5844)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5841..5863)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5852..5868
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5876..5882,5883..5900,5901..5906))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5921..5942
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6059..6076)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6230..6815)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6310..6329)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6986..7777,7778..7846))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7609..7628
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7847..7951)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atggcttcgc tgtaccagag
      961 gttcactggc aagatcaaca cctcgaggtc cttccccgcg cccccggagg cgagtcacct
     1021 cctgggcggc caggggcccg aggaggacgg cggcgcagga gccaagcccc tcggcccgcg
     1081 ggcgcaggcg gcggcgcccc gggagcgcgg cggcggcggc ggcggcgcgg gtggccggcc
     1141 ccggttccag taccaggcgc ggagcgatgg tgacgaggag gacgagctgg tggggagtaa
     1201 ccctccgcag aggaattgga aaggaatagc aattgcactg cttgtcattc tggtcatctg
     1261 ctccttgatc gtcacctcgg tcatacttct gacaccagcg gaagataata gtctgtctca
     1321 aaagaagaag gtcactgtag aagatctctt cagtgaagac ttcaaaattc atgaccccga
     1381 ggctaagtgg ataagtgata cagaattcat ctacagagaa cagaaaggaa cagtgagact
     1441 gtggaatgtt gaaacaaata cttctactgt cttaatagaa ggcaaaaaaa ttgaatcatt
     1501 aagagccatc agatatgaaa tatctccaga tagagagtat gcactttttt catacaatgt
     1561 ggaacccata tatcaacact cgtatactgg atattacgtc ctgagcaaaa ttcctcatgg
     1621 ggatcctcaa agtctggacc caccagaagt cagcaatgca aaacttcagt atgcaggatg
     1681 gggccctaaa ggccaacagc tgatatttat ttttgaaaac aatatctact actgtgcaca
     1741 tgtcgggaaa caggccatcc gtgtggtctc cactggcaag gaaggtgtga tttacaatgg
     1801 cctcagtgac tggctgtatg aagaggagat tttgaagaca cacatcgcac actggtggtc
     1861 tccggatggc acgagactcg cctacgccgc catcaatgat tcccgtgtcc ccatcatgga
     1921 gctcccaact tacaccggct ccatctaccc caccgtgaag ccctaccact atcccaaggc
     1981 tggaagtgag aaccccagca tttccctaca cgttattggc ttaaatggac ccacccatga
     2041 tctggagatg atgccgcctg atgatccacg gatgagggag tactacatca ccatggtgaa
     2101 gtgggccacc agcaccaagg tcgccgtgac ctggctgaac cgggcgcaga acgtgtccat
     2161 cctcaccctc tgcgacgcca ccacgggggt ctgcacgaag aaacacgagg atgaaagtga
     2221 ggcctggctc cacagacaga atgaagaacc tgtgttctcc aaggatggcc gaaagttttt
     2281 cttcatcaga gccatccccc agggaggacg agggaaattc tatcacatca cggtgtcctc
     2341 gtcccagccc aacagcagca acgacaacat ccagtccatc acctccgggg actgggacgt
     2401 gaccaagatc ctagcctacg atgagaaggg gaataagatc tacttcctga gcacggagga
     2461 cctgcctcgg agacgacaac tctacagtgc caacacggtg ggcaacttca acaggcagtg
     2521 cctctcctgt gacctggttg agaactgcac ctacttcagc gcttccttca gccatagcat
     2581 ggacttcttc ctgctcaagt gcgaaggtcc tggtgttcct atggtgacgg tgcacaacac
     2641 aacagataag aaaaaaatgt ttgacctaga aacaaatgaa catgtcaaga aggccataaa
     2701 tgaccgacag atgcctaaag tggaatacag ggacattgag attgatgatt acaacctgcc
     2761 catgcagata ctgaagccag caaccttcac cgacaccacc cactaccctc tgctcctggt
     2821 ggtggatggc accccaggca gccagagtgt ggctgagaag ttcgaggtga gctgggagac
     2881 ggtgatggtg agcagccacg gcgcggtggt ggtaaagtgt gacggccgtg gcagcggctt
     2941 ccaagggacc aagctcctgc acgaagtgag gcggcggctg ggcttgctgg aggagaagga
     3001 ccagatggag gccgtgcgga cgatgctgaa ggagcagtac attgacagga cgcgcgtggc
     3061 cgtgtttggg aaggattacg gtggctacct gagcacctac atcctcccag caaagggaga
     3121 aaatcaaggc cagacattca cctgcggctc tgctctctct ccaataacag acttcaaact
     3181 ctatgcctct gcgttttccg agaggtactt gggcctccat ggacttgaca acagagcata
     3241 cgagatgacc aaggtagccc atcgagtctc cgcgctggaa gaacagcagt tcctgatcat
     3301 tcatcccact gccgatgaaa aaattcattt ccagcacaca gcagaactca ttacacaact
     3361 aattagggga aaggctaatt acagcttaca gatttacccg gacgaaagcc attactttac
     3421 cagctccagc ctcaaacagc atctgtaccg gtccatcatc aacttcttcg tggaatgctt
     3481 caggatccag gacaaactgc tgacagtcac agcgaaagag gacgaggagg aggactaact
     3541 cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     3601 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     3661 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     3721 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     3781 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     3841 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     3901 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     3961 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4021 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     4081 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4141 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     4201 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4261 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     4321 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     4381 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4441 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4501 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4561 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     4621 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     4681 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     4741 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     4801 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     4861 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     4921 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     4981 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     5041 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     5101 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     5161 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     5221 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     5281 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     5341 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     5401 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     5461 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     5521 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     5581 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     5641 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5701 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5761 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     5821 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     5881 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     5941 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     6001 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6061 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6121 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6181 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6241 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6301 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6361 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6421 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6481 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6541 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6601 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6661 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6721 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca
     6781 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     6841 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     6901 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     6961 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7021 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7081 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7141 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7201 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7261 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7321 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7381 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7441 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7501 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7561 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7621 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7681 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     7741 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     7801 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     7861 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     7921 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     7981 tc
//
