LOCUS       sequence-221455-        8553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of ZNF787.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8553)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 8553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: PB09-TRE-ZNF787_G379A
FEATURES             Location/Qualifiers
     source          1..8553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1389..1407
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1425..1443
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1461..1479
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1497..1515
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1533..1551
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1569..1587
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1605..1623
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1668..1692
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2932..2973
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2990..3007
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3064..3081)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3070..3277
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        3354..3565
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3578..3846
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             3880..4623
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             4624..4677
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4684..5082
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    5124..5245
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5161..5180)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5215..5234
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6007..6041
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6532..6548)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6532..6548)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6545..6567)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6556..6572
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6580..6586,6587..6604,6605..6610))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6625..6646
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6763..6780)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6934..7522)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7014..7033)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7693..8484,8485..8553))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8316..8335
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtctttcgtc ttcaagaatt
     1381 ccgtttactc cctatcagtg atagagaacg tatgaagagt ttactcccta tcagtgatag
     1441 agaacgtatg cagactttac tccctatcag tgatagagaa cgtataagga gtttactccc
     1501 tatcagtgat agagaacgta tgaccagttt actccctatc agtgatagag aacgtatcta
     1561 cagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta tcagtgatag
     1621 agaacgtata agctttaggc gtgtacggtg ggcgcctata aaagcagagc tcgtttagtg
     1681 aaccgtcaga tcgcctggag caattccaca acacttttgt cttataccaa ctttccgtac
     1741 cacttcctac cctcgtaaag gtctagagct agcgccacat ggagctgcgg gaagaagcct
     1801 ggtctccggg gccgctggat tctgaggacc agcagatggc cagtcacgag aacccagtgg
     1861 acatcctcat catggatgat gacgacgtcc ccagctggcc tcccaccaag ctgtccccgc
     1921 cccagtctgc gcccccagcc ggcccgcctc cccggccgcg gccgcccgcc ccctacatct
     1981 gcaacgagtg tggcaagagc tttagccact ggtccaagct gacgcggcac cagcgcacgc
     2041 acaccggcga gcggcccaac gcctgcgccg actgcggcaa gaccttctcg cagagctcgc
     2101 acctggtgca gcaccggcgc atccacacgg gcgagaagcc ctacgcctgc ttggagtgcg
     2161 gcaagcgctt cagctggagc tccaacctca tgcagcacca gcgcatccac acgggcgaga
     2221 agccctacac ctgccccgac tgcggccgca gcttcactca gagcaagagc ctggccaagc
     2281 accggcgctc gcacagcggc ctcaagccct tcgtgtgccc gcgctgcggc cgcggcttca
     2341 gccagcccaa gagcctcgcg cgtcacctgc ggctgcaccc ggagctgtcg gggcctggcg
     2401 tggcggccaa ggtgctggcg gctagcgtcc ggcgggccaa ggggcccgaa gaggcggtgg
     2461 cggcggacgg cgagatcgcc atccccgtgg gcgatggcga gggcatcatc gtggtgggcg
     2521 cgccgggcga gggggccgcg gccgcggcag ccatggcggg cgcgggggca aaggccgcgg
     2581 ggccccggtc gcggcgcgcc ccggccccca agccgtacgt gtgtctggag tgcgggaagg
     2641 gcttcgggca cggggccggg ctcctggcgc accagcgggc ccagcacggg gacgggctcg
     2701 gggcggcggg gggcgaggag ccggcccaca tctgcgtgga gtgcggggag ggcttcgtgc
     2761 agggcgccgc gctccggaga cacaagaaga tccacgcggt gggcgcgccc tcggtctgca
     2821 gcagctgcgg acagagctac taccgcgcgg gcggggagga ggaggacgac gacgacgacg
     2881 aggccgcggg cgggcggtgc cccgagtgcc gcggtgggga gggccggtag gggtaagcct
     2941 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaaccggtcc accaccacca
     3001 ccaccactaa ggatccgggg ttggggttgc gccttttcca agcacctgct acatagcggt
     3061 acccctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     3121 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     3181 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     3241 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct tcgaatttaa
     3301 atcggatcca gctgaatcta agtggatctg cgatcgctcc ggtgcccgtc agtgggcaga
     3361 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc
     3421 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     3481 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     3541 caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg
     3601 cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc
     3661 cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag
     3721 accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct
     3781 ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta
     3841 cagatccaag ctgtgaccgg cgcctactct agagccgcca tgtctagact ggacaagagc
     3901 aaagtcataa actctgctct ggaattactc aatggagtcg gtatcgaagg cctgacgaca
     3961 aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac
     4021 aagcgggccc tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccactcc
     4081 tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcataccgc
     4141 tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag
     4201 aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg
     4261 gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag
     4321 gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc
     4381 ccacttctga aacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt
     4441 ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg
     4501 ccgaccgacg cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac
     4561 tttgaccttg atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc
     4621 ggggagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctgaa
     4681 ttcatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca
     4741 atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac
     4801 ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc
     4861 gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc
     4921 ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat
     4981 ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg
     5041 attcgtgaat tgctgccctc tggttatgtg tgggagggct aatgagtcga caatcaacct
     5101 ctggattaca aaatttgtgg gttaacttgt ttattgcagc ttataatggt tacaaataaa
     5161 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     5221 tgtccaaact catcaatgta tcttatcatg tctggaattg actcaaatga tgtcaattag
     5281 tctatcagaa gctatctggt ctcccttccg ggggacaaga catccctgtt taatatttaa
     5341 acagcagtgt tcccaaactg ggttcttata tcccttgctc tggtcaacca ggttgcaggg
     5401 tttcctgtcc tcacaggaac gaagtcccta aagaaacagt ggcagccagg tttagccccg
     5461 gaattgactg gattcctttt ttagggccca ttggtatggc tttttccccg tatcccccca
     5521 ggtgtctgca ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc
     5581 ttccccgtgc ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg
     5641 accggagcgg agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac
     5701 atccctgggg gctttggggg ggggctgtcc ctgatatcta taacaagaaa atatatatat
     5761 aataagttat cacgtaagta gaacatgaaa taacaatata attatcgtat gagttaaatc
     5821 ttaaaagtca cgtaaaagat aatcatgcgt cattttgact cacgcggtcg ttatagttca
     5881 aaatcagtga cacttaccgc attgacaagc acgcctcacg ggagctccaa gcggcgactg
     5941 agatgtccta aatgcacagc gacggattcg cgctatttag aaagagagag caatatttca
     6001 agaatgcatg cgtcaatttt acgcagacta tctttctagg gttaatctag ctgcatcagg
     6061 atcatatcgt cgggtctttt ttccggctca gtcatcgccc aagctggcgc tatctgggca
     6121 tcggggagga agaagcccgt gccttttccc gcgaggttga agcggcatgg aaagagtttg
     6181 ccgaggatga ctgctgctgc attgacgttg agcgaaaacg cacgtttacc atgatgattc
     6241 gggaaggtgt ggccatgcac gcctttaacg gtgaactgtt cgttcaggcc acctgggata
     6301 ccagttcgtc gcggcttttc cggacacagt tccggatggt cagcccgaag cgcatcagca
     6361 acccgaacaa taccggcgac agccggaact gccgtgccgg tgtgcagatt aatgacagcg
     6421 gtgcggcgct gggatattac gtcagcgagg acgggtatcc tggctggatg ccgcagaaat
     6481 ggacatggat accccgtgag ttacccggcg ggcgcgcttg gcgtaatcat ggtcatagct
     6541 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     6601 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     6661 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     6721 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     6781 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6841 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6901 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6961 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     7021 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7081 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     7141 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     7201 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7261 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     7321 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     7381 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7441 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7501 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7561 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7621 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7681 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7741 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7801 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7861 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7921 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7981 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     8041 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     8101 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     8161 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     8221 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     8281 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     8341 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     8401 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     8461 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8521 aataagggcg acacggaaat gttgaatact cat
//
