LOCUS       sequence-22149-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Duennwald ML, Jagadish S, Muchowski PJ, Lindquist S
  TITLE     Flanking sequences profoundly alter polyglutamine toxicity in yeast.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50.
  PUBMED    16832050
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 18. 103(29):11045-50."
COMMENT     Sequence Label: GAL FLAG46QDProCFPp303
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             5..28
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(266..294)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     889..909
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 catggactac aaggacgacg atgacaagct ggcgaccctg gaaaagctga tgaaggcctt
       61 cgagtccctc aaaagcttcc aacagcagca acagcaacaa cagcagcaac agcaacaaca
      121 gcagcaacag caacaacagc agcaacagca gcaacagcaa caacagcagc aacagcaaca
      181 acagcagcaa cagcaacaac agcagcaaca gcaacaactg cagcccgggg gatccctgag
      241 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
      301 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
      361 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
      421 tactttgact tggggtgttc aatgcttttc aagataccca gatcatatga aacggcatga
      481 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
      541 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
      601 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
      661 atacaactat atttcacaca atgtatacat cactgcagac aaacaaaaga atggaatcaa
      721 agctcatttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta
      781 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
      841 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga
      901 gtttgtaaca gct
//
