LOCUS       sequence-22151-e         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Duennwald ML, Jagadish S, Muchowski PJ, Lindquist S
  TITLE     Flanking sequences profoundly alter polyglutamine toxicity in yeast.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50.
  PUBMED    16832050
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 18. 103(29):11045-50."
COMMENT     Sequence Label: GAL FLAG103QDProGFPp303
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             5..28
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(437..465)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 atgggactac aaggacgacg atgacaagct ggcgaccctg gaaaagctga tgaaggcctt
       61 cgagtccctc aaaagcttcc aacagcagca acagcaacaa cagcagcaac agcaacaaca
      121 gcagcaacag caacaacagc agcaacagca acaacagcag caacagcaac aacagcagca
      181 acagcaacaa cagcagcaac agcaacaaca gcagcaacag caacaacagc agcaacagca
      241 acaacagcag caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca
      301 gcagcaacag caacaacagc agcaacagca acaacagcag caacagcaac aacagcagca
      361 acagcaacaa cagcagcaac agcaacaact gcagcccggg ggatccctga gtaaaggaga
      421 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
      481 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
      541 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttgac
      601 ttggggtgtt caatgctttt caagataccc agatcatatg aaacggcatg actttttcaa
      661 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggga
      721 ctacaagaca cgtgctgaag t
//
