LOCUS       sequence-221518-         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of gRNA targeting ALG5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Narimatsu Y, Joshi HJ, Zhang Y, Gomes C, Chen YH, Lorenzetti F, 
            Furukawa S, Schjoldager K, Hansen L, Clausen H, Bennett EP, Wandall 
            HH
  TITLE     A validated gRNA library for CRISPR/Cas9 targeting of the human 
            glycosyltransferase genome.
  JOURNAL   Glycobiology. 2018 Jan 5. pii: 4791732. doi: 10.1093/glycob/cwx101.
  PUBMED    29315387
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Glycobiology. 2018 Jan 5. pii: 4791732. doi: 
            10.1093/glycob/cwx101."
COMMENT     Sequence Label: gh259
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     106..125
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        204..279
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(418..435)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(608..627)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa
       61 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt
      121 accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac
      181 accgtaggtg agtcccatat gctgttttag agctagaaat agcaagttaa aataaggcta
      241 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttggta cctggagcac
      301 aagactggcc tcatgggcct tccgctcact gcccgctttc cagtcgggaa acctgtcgtg
      361 ccagctgcat taacatggtc atagctgttt ccttgcgtat tgggcgctct ccgcttcctc
      421 gctcactgac tcgctgcgct cggtcgttcg ggtaaagcct ggggtgccta atgagcaaaa
      481 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      541 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      601 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      661 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      721 catagctcac gctgtaggta tctcag
//
