LOCUS       sequence-22152-e         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Duennwald ML, Jagadish S, Muchowski PJ, Lindquist S
  TITLE     Flanking sequences profoundly alter polyglutamine toxicity in yeast.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50.
  PUBMED    16832050
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 18. 103(29):11045-50."
COMMENT     Sequence Label: MET FLAG25QDProCFPp303
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..26
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     11..27
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(44..291)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     257..275
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(341..361)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgggncncct cgaggtcgac ggtatcgata agcttgatcg gccgcaaatt aaagccttcg
       61 agcgtcccaa aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc
      121 tgtacagaaa aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta
      181 taaaaaaata aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag
      241 cggatgtggg gggagggcgt gaatgtaagc gtgacataac taattacatg actcgagcta
      301 tttgtatagt tcatccatgc catgtgtaat cccagcagct gttacaaact caagaaggac
      361 catgtggtct ctcttttcgt tgggatcttt cgaaagggca gattgtgtgg acaggtaatg
      421 gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat aatggtctgc
      481 tagttgaacg cttccatctt caatgttgtg tctaattttg aaatgagctt tgattccatt
      541 cttttgtttg tctgcagtga tgtatacatt gtgtgaaata tagttgtatt ccaatttgtg
      601 tccaagaatg tttccatctt ctttaaaatc aatacctttt aactcgattc tattaacaag
      661 ggtatcacct tcaaacttga cttcagcacg tgtcttgtag ttcccgtcat ctttgaaaaa
      721 tatagttctt tcctgnna
//
