LOCUS       sequence-221530-        5074 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ProC driven mCherry expression with synTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Cooper KG, Chong A, Starr T, Finn CE, Steele-Mortimer O
  TITLE     Predictable, Tunable Protein Production in Salmonella for Studying 
            Host-Pathogen Interactions.
  JOURNAL   Front Cell Infect Microbiol. 2017 Nov 16;7:475. doi: 
            10.3389/fcimb.2017.00475. eCollection 2017.
  PUBMED    29201859
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Infect Microbiol."; date: "2017-11-16"; volume: "7:475. doi"; pages:
            " 10.3389/fcimb.2017.00475. eCollection 2017"
COMMENT     Sequence Label: pCON1-ProC.mCherry
FEATURES             Location/Qualifiers
     source          1..5074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     30..48
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             282..992
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(427..445)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     695..714
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      1013..1084
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1100..1127
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     oriT            1741..1828
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     primer_bind     complement(3141..3158)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3312..3900)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3392..3411)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4071..4862,4863..4931))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4694..4713
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4932..5036)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
       61 aagaattcga gctcggtacc cgggcacagc taacaccacg tcgtccctat ctgctgccct
      121 aggtctatga gtggttgctg gataacttta cgggcatgca taaggctcgt atgatatatt
      181 cagggagacc acaacggttt ccctctacaa ataattttgt ttaactttta ctagagtcac
      241 acaggaaagt actagtctag atttaagaag gagatataca tatggtgagc aagggcgagg
      301 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
      361 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
      421 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
      481 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
      541 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
      601 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
      661 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
      721 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
      781 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
      841 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
      901 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
      961 ccaccggcgg catggacgag ctgtacaagt aagcatgcaa gcttccaggc atcaaataaa
     1021 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     1081 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ataaagcttt
     1141 aatgcggtag tttatcacag ttaaattgct aacgcagtca ggcaccgtgt atgaaatcta
     1201 acaatgcgct catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg
     1261 ttatgccggt actgccgggc ctcttgcggg atatcgtcca ttccgacagc atcgccagtc
     1321 actatggcgt gctgctagcg cttttagccg ctttagcggc ctttccccct acccgaaggg
     1381 tgggggcgcg tgtgcagccc cgcagggcct gtctcggtcg atcattcagc ccggctcatc
     1441 cttctggcgt ggcggcagac cgaacaaggc gcggtcgtgg tcgcgttcaa ggtacgcatc
     1501 cattgccgcc atgagccgat cctccggcca ctcgctgctg ttcaccttgg ccaaaatcat
     1561 ggcccccacc agcaccttgc gccttgtttc gttcttgcgc tcttgctgct gttcccttgc
     1621 ccgcacccgc tgaatttcgg cattgattcg cgctcgttgt tcttcgagct tggccagccg
     1681 atccgccgcc ttgttgctcc ccttaaccat cttgacaccc cattgttaat gtgctgtctc
     1741 gtaggctatc atggaggcac agcggcggca atcccgaccc tactttgtag gggagggcgc
     1801 acttaccggt ttctcttcga gaaactggcc taacggccac ccttcgggcg gtgcgctctc
     1861 cgagggccat tgcatggagc cgaaaagcaa aagcaacagc gaggcagcat ggcgatttat
     1921 caccttacgg cgaaaaccgg cagcaggtcg ggcggccaat cggccagggc caaggccgac
     1981 tacatccagc gcgaaggcaa gtatgcccgc gacatggatg aagtcttgca cgccgaatcc
     2041 gggcacatgc cggagttcgt cgagcggccc gccgactact gggatgctgc cgacctgtat
     2101 gaacgcgcca atgggcggct gttcaaggag gtcgaatttg ccctgccggt cgagctgacc
     2161 ctcgaccagc agaaggcgct ggcgtccgag ttcgcccagc acctgaccgg tgccgagcgc
     2221 ctgccgtata cgctggccat ccatgccggt ggcggcgaga acccgcactg ccacctgatg
     2281 atctccgagc ggatcaatga cggcatcgag cggcccgccg ctcagtggtt caagcggtac
     2341 aacggcaaga ccccggagaa gggcggggca cagaagaccg aagcgctcaa gcccaaggca
     2401 tggcttgagc agacccgcga ggcatgggcc gaccatgcca accgggcatt agagcgggct
     2461 ggccacgacg cccgcattga ccacagaaca cttgaggcgc agggcatcga gcgcctgccc
     2521 ggtgttcacc tggggccgaa cgtggtggag atggaaggcc ggggcatccg caccgaccgg
     2581 gcagacgtgg ccctgaacat cgacaccgcc aacgcccaga tcatcgactt acaggaatac
     2641 cgggaggcaa tagaccatga acgcaatcga cagagtgaag aaatccagag gcatcaacga
     2701 gttagcggag cagatcgaac cgctggccca gagcatggcg acactggccg acgaagcccg
     2761 gcaggtcatg agccagaccc agcaggccag cgaggcgcag gcggcggagt ggctgaaagc
     2821 ccagcgccag acaggggcgg catgggtgga gctggccaaa gagttgcggg aggtagccgc
     2881 cgaggtgagc agcgccgcgc agagcgcccg gagcgcgtcg cgggggtggc actggaagct
     2941 atggctaacc gtgatgctgg cttccatgat gcctacggtg gtgctgctga tcgcatcgtt
     3001 gctcttgctc gacctgacgc cactgacaac cgaggacggc tcgatctggc tgcgcttggt
     3061 ggcccgatga agaacgacag gactttgcag gccataggcc gacagctcaa ggccatgggc
     3121 tgtgagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3181 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     3241 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3301 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     3361 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3421 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3481 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3541 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3601 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3661 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3721 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     3781 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3841 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3901 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3961 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     4021 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     4081 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     4141 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     4201 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     4261 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     4321 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     4381 attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     4441 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     4501 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     4561 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     4621 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4681 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     4741 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     4801 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     4861 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     4921 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     4981 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     5041 tttccccgaa aagtgccacc tgacgtctaa gaaa
//
