LOCUS       sequence-221543-        5803 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 400 / 490, Emission: 468 
            / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5803)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 5803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-VASP-5
FEATURES             Location/Qualifiers
     source          1..5803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             553..1266
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
     polyA_signal    2531..2652
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2568..2587)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2622..2641
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2659..3114)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2796..2817)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3008..3027
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3141..3245
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3247..3604
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3247..3438
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3270..3290)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3455..3590
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3639..4433
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3639..4433
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3693..3712)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4303..4322
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4621..4640)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4665..4712
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5041..5629
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5530..5549
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatgagcaa gggcgccgag ctgttcaccg gcatcgtgcc catcctgatc
      601 gagctgaatg gcgatgtgaa tggccacaag ttcagcgtga gcggcgaggg cgagggcgat
      661 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcctgtgccc
      721 tggcccaccc tggtggccac cctgagctac ggcgtgcagt gcttctcacg ctaccccgat
      781 cacatgaagc agcacgactt cttcaagagc gccatgcctg agggctacat ccaggagcgc
      841 accatcttct tcgaggatga cggcaactac aagacgcgcg ccgaggtgaa gttcgagggc
      901 gataccctgg tgagtcgcat cgagctgacc ggcactgatt tcaaggagga tggcaacatc
      961 ctgggcaata agatggagta caactacaac gccaccaatg tgtacatcgt ggccgacaag
     1021 gccaggaatg gcatcaaggt gaacttcaag gtccgccaca acatcaagga tggcagcgtg
     1081 cagctggccg accactacca gcagaatacc cccatcggcg atggccctgt gctgctgccc
     1141 gataaccact acctgtccac ccagagcgcc ctgtccaagg accccaacga gaagcgcgat
     1201 cacatgatct acctcgagtt cgtgaccgcc gccgccatca cccacggcat ggatgagctg
     1261 tacaagtccg gactcagatc tatgagcgag acggtcatct gttccagccg ggctactgtg
     1321 atgctttatg atgacagcaa caagcgatgg ctccctgctg gcactggtcc ccaggccttc
     1381 agccgcgtcc agatatacca caaccccact gctaactcct tccgagttgt tggccgcaag
     1441 atgcagccgg atcagcaggt ggttatcaac tgtgccatca ttcggggtgt caagtacaat
     1501 caggccactc ccatcttcca tcagtggcga gatgcccgcc aggtctgggg cctcaacttc
     1561 ggcagcaagg aggacgccat acagtttgca acaggcatgg ccaacgccct agaggccttg
     1621 gaaggaggtg ggcctccccc agccccagca ccccctgcct ggtctgccca gaatggtccc
     1681 tccccagagg agctggaaca acagaaaagg cagccggagc atatggagcg ccgggtctcc
     1741 aatgcaggag gcccacctgc tcccccagct gggggccctc ctccacctcc aggacctccc
     1801 cctcctccag gtcccccccc acccccaggt ctgccctcct caggggtatc tggggcaggt
     1861 catggagcag gggcagcccc accccctgca cccccactcc ctacaacaca gggccccaat
     1921 agtgggggtt ccggggcccc aggcctggct gctgccattg ctggagccaa actcaggaaa
     1981 gtgagcaagc aggaggaggc ctctgggggg cccctggccc ccaaagctga gaacagtcga
     2041 agcactggtg gggggcttat ggaagagatg aacgccatgc tggcccggag aagaaaagcc
     2101 acacaggttg gggagaagcc ccccaaagac gagtcagcca gtgaggagtc agaggcccga
     2161 ctccctgccc agagtgaacc tgtgagaaga ccctgggaga agaacagcac aaccttgcca
     2221 aggatgaagt cgtcctcttc tgtgactacc tccgaggccc acccctcgac gccctgctcc
     2281 agtgatgact ccgacttgga gagggtgaag caggagcttc tggaagaggt gcggaaggag
     2341 ctacagaaaa tgaaagagga aatcatcgaa gtctttgtcc aggagctgag gaagcggggt
     2401 tctccttgag cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt
     2461 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     2521 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     2581 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     2641 caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     2701 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     2761 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     2821 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     2881 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     2941 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     3001 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     3061 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
     3121 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     3181 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     3241 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     3301 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     3361 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3421 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     3481 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     3541 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     3601 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     3661 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     3721 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     3781 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     3841 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     3901 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     3961 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     4021 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     4081 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     4141 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
     4201 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     4261 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     4321 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     4381 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     4441 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     4501 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     4561 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
     4621 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     4681 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
     4741 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
     4801 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
     4861 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca
     4921 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4981 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     5041 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     5101 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     5161 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     5221 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     5281 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     5341 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     5401 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     5461 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     5521 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     5581 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5641 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     5701 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat
     5761 caattacggg gtcattagtt catagcccat atatggagtt ccg
//
