LOCUS       sequence-221548-        5939 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 400 / 490, Emission: 468 / 
            511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5939)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 5939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-Myotilin-C-14
FEATURES             Location/Qualifiers
     source          1..5939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             249..962
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
     polyA_signal    2628..2749
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2665..2684)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2719..2738
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2756..3211)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2893..2914)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3105..3124
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3238..3342
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3367..3387)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3411..3621
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        3433..3629
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3480..3615
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3664..4458
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3664..4458
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3718..3737)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4328..4347
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4646..4665)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4690..4737
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5066..5654
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5555..5574
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 tcgccaccat gagcaagggc gccgagctgt tcaccggcat cgtgcccatc ctgatcgagc
      301 tgaatggcga tgtgaatggc cacaagttca gcgtgagcgg cgagggcgag ggcgatgcca
      361 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgcct gtgccctggc
      421 ccaccctggt ggccaccctg agctacggcg tgcagtgctt ctcacgctac cccgatcaca
      481 tgaagcagca cgacttcttc aagagcgcca tgcctgaggg ctacatccag gagcgcacca
      541 tcttcttcga ggatgacggc aactacaaga cgcgcgccga ggtgaagttc gagggcgata
      601 ccctggtgag tcgcatcgag ctgaccggca ctgatttcaa ggaggatggc aacatcctgg
      661 gcaataagat ggagtacaac tacaacgcca ccaatgtgta catcgtggcc gacaaggcca
      721 ggaatggcat caaggtgaac ttcaaggtcc gccacaacat caaggatggc agcgtgcagc
      781 tggccgacca ctaccagcag aataccccca tcggcgatgg ccctgtgctg ctgcccgata
      841 accactacct gtccacccag agcgccctgt ccaaggaccc caacgagaag cgcgatcaca
      901 tgatctacct cgagttcgtg accgccgccg ccatcaccca cggcatggat gagctgtaca
      961 agtccggaag tgctgggagc gcaagcggaa gcggaggctc cggttttaac tacgaacgtc
     1021 caaaacactt catccagtcc caaaacccat gtggctccag attgcagcct cctggaccag
     1081 aaacctccag cttctctagc cagaccaaac agtcttccat tatcatccag ccccgccagt
     1141 gtacagagca aagattttct gcctcctcaa cactgagctc tcacatcacc atgtcctcct
     1201 ctgctttccc tgcttctccc cagcagcatg ctggctccaa cccaggccaa agggttacaa
     1261 ccacctataa ccagtcccca gccagcttcc tcagctccat attaccatca cagcctgatt
     1321 acaatagcag taaaatccct tccgctatgg attccaacta tcaacagtcc tcagctggcc
     1381 aacctataaa tgcaaagcca tcccaaactg caaatgctaa gcccatacca agaactcctg
     1441 atcatgaaat acaaggatca aaagaagctt tgattcaaga tttggaaaga aagctgaaat
     1501 gcaaggacac ccttcttcat aatggaaatc aacgtctaac atatgaagag aagatggctc
     1561 gcagattgct aggaccacag aatgcagctg ctgtgtttca agctcaggat gacagtggtg
     1621 cacaagactc gcagcaacac aactcagaac atgcgcgact gcaagttcct acatcacaag
     1681 taagaagtag atcaacctca aggggagatg tgaatgatca ggatgcaatc caggagaaat
     1741 tttacccacc acgtttcatt caagtgccag agaacatgtc gattgatgaa ggaagattct
     1801 gcagaatgga cttcaaagtg agtggactgc cagctcctga tgtgtcatgg tatctaaatg
     1861 gaagaacagt tcaatcagat gatttgcaca aaatgatagt gtctgagaag ggtcttcatt
     1921 cactcatctt tgaagtagtc agagcttcag atgcaggggc ttatgcatgt gttgccaaga
     1981 atagagcagg agaagccacc ttcactgtgc agctggatgt ccttgcaaaa gaacataaaa
     2041 gagcaccaat gtttatctac aaaccacaga gcaaaaaagt tttagaggga gattcagtga
     2101 aactagaatg ccagatctcg gctatacctc caccaaagct tttctggaaa agaaataatg
     2161 aaatggtaca attcaacact gaccgaataa gcttatatca agataacact ggaagagtta
     2221 ctttactgat aaaagatgta aacaagaaag atgctgggtg gtatactgtg tcagcagtta
     2281 atgaagctgg agtgactaca tgtaacacaa gattagacgt tacggcacgt ccaaaccaaa
     2341 ctcttccagc tcctaagcag ttacgggttc gaccaacatt cagcaaatat ttagcactta
     2401 atgggaaagg tttgaatgta aaacaagctt ttaacccaga aggagaattt cagcgtttgg
     2461 cagctcaatc tggactctat gaaagtgaag aactttaagg atccaccgga tctagataac
     2521 tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     2581 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     2641 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     2701 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt
     2761 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     2821 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     2881 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     2941 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     3001 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     3061 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     3121 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     3181 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     3241 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     3301 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc
     3361 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     3421 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     3481 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     3541 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     3601 gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt
     3661 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     3721 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     3781 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     3841 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     3901 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     3961 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     4021 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     4081 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     4141 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc
     4201 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     4261 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     4321 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     4381 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     4441 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     4501 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     4561 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     4621 tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
     4681 gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata
     4741 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg
     4801 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc
     4861 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata
     4921 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     4981 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5041 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5101 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5161 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     5221 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     5281 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     5341 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     5401 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     5461 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     5521 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     5581 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     5641 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     5701 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     5761 ccatgcatta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     5821 gagttccgcg ttacataact tacggtaaat ggcccgcctg gcattatgcc cagtacatga
     5881 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatg
//
