LOCUS       sequence-22155-e         599 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Duennwald ML, Jagadish S, Muchowski PJ, Lindquist S
  TITLE     Flanking sequences profoundly alter polyglutamine toxicity in yeast.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50.
  PUBMED    16832050
REFERENCE   2  (bases 1 to 599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 18. 103(29):11045-50."
COMMENT     Sequence Label: MET FLAG103QDProCFPp303
FEATURES             Location/Qualifiers
     source          1..599
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(397..420)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(440..456)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(440..456)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ttnttctcct ttactcaggg atcccccggg ctgcagttgt tgctgttgct gctgttgttg
       61 ctgttgctgc tgttgttgct gttgctgctg ttgttgctgt tgctgctgtt gttgctgttg
      121 ctgctgttgt tgctgttgct gctgttgttg ctgttgctgc tgttgttgct gttgctgctg
      181 ttgttgctgt tgctgctgtt gttgctgttg ctgctgttgt tgctgttgct gctgttgttg
      241 ctgttgctgc tgttgttgct gttgctgctg ttgttgctgt tgctgctgtt gttgctgttg
      301 ctgctgttgt tgctgttgct gctgttgttg ctgttgctgc tgttggaagc ttttgaggga
      361 ctcgaaggcc ttcatcagct tttccagggt cgccagcttg tcatcgtcgt ccttgtagtc
      421 catggcgagt tctagggggg atccactagt tctagagtat ggatgggggt aatagaattg
      481 tatctatgta tctgacgacc ctgtattaca cgaaggaaat agtagatgaa aagacaagag
      541 agcaagaaaa aggaaaaact tcctttctaa cagacgcttt acttaacctt atatatatc
//
