LOCUS       sequence-221553-        6220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 400 / 490, Emission: 
            468 / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6220)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-ALK5-N-13
FEATURES             Location/Qualifiers
     source          1..6220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      555..564
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2109..2825
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
     polyA_signal    2948..3069
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2985..3004)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3039..3058
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3076..3531)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3213..3234)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3425..3444
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3558..3662
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3664..4021
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3664..3855
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3687..3707)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3872..4007
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4056..4850
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4056..4850
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4110..4129)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4720..4739
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5038..5057)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5082..5129
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5458..6046
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5947..5966
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctgccacc atggaggcgg cggtcgctgc tccgcgtccc cggctgctcc
      601 tcctcgtgct ggcggcggcg gcggcggcgg cggcggcgct gctcccgggg gcgacggcgt
      661 tacagtgttt ctgccacctc tgtacaaaag acaattttac ttgtgtgaca gatgggctct
      721 gctttgtctc tgtcacagag accacagaca aagttataca caacagcatg tgtatagctg
      781 aaattgactt aattcctcga gataggccgt ttgtatgtgc accctcttca aaaactgggt
      841 ctgtgactac aacatattgc tgcaatcagg accattgcaa taaaatagaa cttccaacta
      901 ctgtaaagtc atcacctggc cttggtcctg tggaactggc agctgtcatt gctggaccag
      961 tgtgcttcgt ctgcatctca ctcatgttga tggtctatat ctgccacaac cgcactgtca
     1021 ttcaccatcg agtgccaaat gaagaggacc cttcattaga tcgccctttt atttcagagg
     1081 gtactacgtt gaaagactta atttatgata tgacaacgtc aggttctggc tcaggtttac
     1141 cattgcttgt tcagagaaca attgcgagaa ctattgtgtt acaagaaagc attggcaaag
     1201 gtcgatttgg agaagtttgg agaggaaagt ggcggggaga agaagttgct gttaagatat
     1261 tctcctctag agaagaacgt tcgtggttcc gtgaggcaga gatttatcaa actgtaatgt
     1321 tacgtcatga aaacatcctg ggatttatag cagcagacaa taaagacaat ggtacttgga
     1381 ctcagctctg gttggtgtca gattatcatg agcatggatc cctttttgat tacttaaaca
     1441 gatacacagt tactgtggaa ggaatgataa aacttgctct gtccacggcg agcggtcttg
     1501 cccatcttca catggagatt gttggtaccc aaggaaagcc agccattgct catagagatt
     1561 tgaaatcaaa gaatatcttg gtaaagaaga atggaacttg ctgtattgca gacttaggac
     1621 tggcagtaag acatgattca gccacagata ccattgatat tgctccaaac cacagagtgg
     1681 gaacaaaaag gtacatggcc cctgaagttc tcgatgattc cataaatatg aaacattttg
     1741 aatccttcaa acgtgctgac atctatgcaa tgggcttagt attctgggaa attgctcgac
     1801 gatgttccat tggtggaatt catgaagatt accaactgcc ttattatgat cttgtacctt
     1861 ctgacccatc agttgaagaa atgagaaaag ttgtttgtga acagaagtta aggccaaata
     1921 tcccaaacag atggcagagc tgtgaagcct tgagagtaat ggctaaaatt atgagagaat
     1981 gttggtatgc caatggagca gctaggctta cagcattgcg gattaagaaa acattatcgc
     2041 aactcagtca acaggaaggc atcaaaatgg gttccggggg tgcgggcggc ggaggaccgg
     2101 tcgccaccat gagcaagggc gccgagctgt tcaccggcat cgtgcccatc ctgatcgagc
     2161 tgaatggcga tgtgaatggc cacaagttca gcgtgagcgg cgagggcgag ggcgatgcca
     2221 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgcct gtgccctggc
     2281 ccaccctggt ggccaccctg agctacggcg tgcagtgctt ctcacgctac cccgatcaca
     2341 tgaagcagca cgacttcttc aagagcgcca tgcctgaggg ctacatccag gagcgcacca
     2401 tcttcttcga ggatgacggc aactacaaga cgcgcgccga ggtgaagttc gagggcgata
     2461 ccctggtgag tcgcatcgag ctgaccggca ctgatttcaa ggaggatggc aacatcctgg
     2521 gcaataagat ggagtacaac tacaacgcca ccaatgtgta catcgtggcc gacaaggcca
     2581 ggaatggcat caaggtgaac ttcaaggtcc gccacaacat caaggatggc agcgtgcagc
     2641 tggccgacca ctaccagcag aataccccca tcggcgatgg ccctgtgctg ctgcccgata
     2701 accactacct gtccacccag agcgccctgt ccaaggaccc caacgagaag cgcgatcaca
     2761 tgatctacct cgagttcgtg accgccgccg ccatcaccca cggcatggat gagctgtaca
     2821 agtaaagcgg ccgcgactct agatcataat cagccatacc acatttgtag aggttttact
     2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3061 tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     3121 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     3181 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     3241 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     3301 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     3361 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     3421 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     3481 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
     3541 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     3601 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     3661 gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     3721 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     3781 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3841 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3901 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3961 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     4021 agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     4081 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     4141 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     4201 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
     4261 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     4321 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     4381 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     4441 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     4501 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     4561 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
     4621 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     4681 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     4741 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     4801 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     4861 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     4921 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     4981 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga
     5041 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     5101 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     5161 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     5221 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     5281 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt
     5341 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     5401 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5461 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5521 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5581 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     5641 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5701 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5761 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5821 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     5881 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5941 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6001 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6061 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6121 atcccctgat tctgtggata accgtattac cgccatgcat tagttattaa tagtaatcaa
     6181 ttacggggtc attagttcat agcccatata tggagttccg
//
