LOCUS       sequence-221556-        7091 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 400 / 490, Emission: 468
            / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7091)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 7091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-Integrin-Beta3-N-18
FEATURES             Location/Qualifiers
     source          1..7091
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2980..3696
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
     polyA_signal    3819..3940
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3856..3875)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3910..3929
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3947..4402)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4084..4105)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4296..4315
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4429..4533
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4535..4892
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4535..4726
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4558..4578)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4743..4878
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4927..5721
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4927..5721
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4981..5000)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5591..5610
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5909..5928)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5953..6000
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6329..6917
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6818..6837
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagac cgccatgcga gcgcagtggc cgggacaact ctgggccgct
      601 ctgctggcgc tgggggcgct ggcgggcgtt gttgttggag agtccaacat ctgtaccaca
      661 cgaggcgtga actcctgcca gcagtgtctg gctgtgagtc ctgtgtgtgc ctggtgctca
      721 gatgagactt tgtctcaggg ctcaccccga tgtaacctga aggagaacct gctgaaggac
      781 aattgtgctc cagagtctat tgagttccca gtcagtgagg cccagatcct ggaggctagg
      841 ccactcagca gcaagggctc tggaagcagc gcccagatca ctcaagtcag ccctcagagg
      901 attgcccttc gactacggcc agatgattcg aagatcttct cacttcaagt gcggcaggtg
      961 gaggattacc ccgtggacat ctactacttg atggacctgt ctttctccat gaaggatgat
     1021 ctgtccagca tccagaccct gggtaccaag ttggcctctc agatgcgcaa gcttactagc
     1081 aaccttcgga tcggctttgg ggccttcgtg gacaagcctg tatcgccgta catgtacatc
     1141 tccccaccac aggcaatcaa aaacccctgt tacaatatga agaatgcctg cttgcccatg
     1201 tttggctaca aacacgtgct gacgctaacc gaccaggtgt cccgcttcaa tgaagaagtg
     1261 aagaaacaga gcgtgtcccg taatcgagat gccccagagg gcggctttga cgccatcatg
     1321 caggctacag tatgtgatga aaaaattggc tggaggaatg acgcatccca tttgctagtg
     1381 tttaccacgg atgccaagac ccatattgcc ctggatggaa ggctggcagg cattgtcctg
     1441 cccaatgatg ggcactgtca cattggcacc gacaaccact actctgcctc cactaccatg
     1501 gactacccat ctctggggct gatgactgag aaactatccc agaaaaacat taacttgatc
     1561 tttgcagtga ctgaaaatgt cgtcagcctt taccagaatt atagtgagct cattcctggg
     1621 accacagtgg gagtcctgtc tgatgactca agcaacgtcc tccagctcat tgttgatgct
     1681 tacgggaaaa tccgctctaa agtggagctg gaagtacgtg acctgccgga agaactgtca
     1741 ctgtccttca atgccacctg cctcaacaac gaggttatcc cgggcctcaa gtcttgtgtg
     1801 ggcctcaaga ttggagacac ggtgagcttt agtatcgagg ccaaggtgcg tggctgcccc
     1861 caggagaagg agcagtcttt cactatcaag cctgtgggct ttaaggacag cctcaccgtc
     1921 caggtgacct tcgactgtga ctgtgcctgc caggcctttg cccagccttc cagcccacgc
     1981 tgcaacaatg ggaacgggac ttttgagtgt ggggtgtgcc gctgtgacca gggctggctg
     2041 gggtccatgt gtgagtgctc tgaggaggat taccgaccct ctcagcagga agagtgcagc
     2101 cccaaggagg gccagcccat ctgcagccag cggggagagt gcctctgtgg ccagtgtgtc
     2161 tgccatagca gcgacttcgg caagatcact ggcaagtact gtgagtgcga tgacttctcc
     2221 tgcgtccgct acaaagggga gatgtgttcc ggccatgggc aatgtaactg tggggactgc
     2281 gtgtgtgact cggactggac tggctactac tgcaactgta ctacacgcac tgacacctgc
     2341 atgtccacca atgggctgct gtgcagcggc cggggcaact gcgagtgcgg cagctgtgtg
     2401 tgcgtccagc caggctccta tggagacacc tgtgagaagt gccccacctg cccagatgcc
     2461 tgctccttta agaaggagtg tgtggagtgt aagaagttca accggggaac gctccatgaa
     2521 gaaaacacct gcagccgcta ctgccgggac gacatcgagc aggtgaaaga gctgacggat
     2581 actggcaaaa acgccgtgaa ttgtacctac aagaacgagg atgactgtgt cgtcagattc
     2641 cagtactacg aagacaccag tgggagggca gtcctctatg tggtggaaga gcctgagtgt
     2701 cccaagggtc ctgatatcct ggtggtcctg ctgtcagtga tgggggccat cctgctcatt
     2761 ggccttgcta ctctgctcat ctggaagcta ctcatcacca tccatgaccg gaaggaattt
     2821 gctaaatttg aggaagaacg agccagagcc aagtgggaca cagcaaacaa cccgctgtat
     2881 aaagaggcca cctccacctt caccaatatc acctaccggg ggactggtag cgcatccggg
     2941 ggtgcgtcag gcggcagcgg atctggaccg gtcgccacca tgagcaaggg cgccgagctg
     3001 ttcaccggca tcgtgcccat cctgatcgag ctgaatggcg atgtgaatgg ccacaagttc
     3061 agcgtgagcg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     3121 tgcaccaccg gcaagctgcc tgtgccctgg cccaccctgg tggccaccct gagctacggc
     3181 gtgcagtgct tctcacgcta ccccgatcac atgaagcagc acgacttctt caagagcgcc
     3241 atgcctgagg gctacatcca ggagcgcacc atcttcttcg aggatgacgg caactacaag
     3301 acgcgcgccg aggtgaagtt cgagggcgat accctggtga gtcgcatcga gctgaccggc
     3361 actgatttca aggaggatgg caacatcctg ggcaataaga tggagtacaa ctacaacgcc
     3421 accaatgtgt acatcgtggc cgacaaggcc aggaatggca tcaaggtgaa cttcaaggtc
     3481 cgccacaaca tcaaggatgg cagcgtgcag ctggccgacc actaccagca gaataccccc
     3541 atcggcgatg gccctgtgct gctgcccgat aaccactacc tgtccaccca gagcgccctg
     3601 tccaaggacc ccaacgagaa gcgcgatcac atgatctacc tcgagttcgt gaccgccgcc
     3661 gccatcaccc acggcatgga tgagctgtac aagtaaagcg gccgcgactc tagatcataa
     3721 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     3781 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     3841 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     3901 attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt
     3961 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     4021 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     4081 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     4141 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     4201 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     4261 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     4321 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     4381 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     4441 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4501 taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga
     4561 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4621 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     4681 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     4741 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     4801 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     4861 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg
     4921 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     4981 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     5041 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     5101 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     5161 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     5221 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     5281 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     5341 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     5401 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
     5461 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     5521 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     5581 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     5641 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     5701 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     5761 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     5821 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     5881 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
     5941 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc
     6001 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
     6061 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
     6121 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat
     6181 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6241 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6301 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6361 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6421 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6481 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6541 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6601 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6661 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6721 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6781 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6841 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6901 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6961 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     7021 ccgccatgca ttagttatta atagtaatca attacggggt cattagttca tagcccatat
     7081 atggagttcc g
//
