LOCUS       sequence-22156-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Duennwald ML, Jagadish S, Muchowski PJ, Lindquist S
  TITLE     Flanking sequences profoundly alter polyglutamine toxicity in yeast.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50.
  PUBMED    16832050
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 18. 103(29):11045-50."
COMMENT     Sequence Label: MET FLAG103QDProCFPp303
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..21
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6..22
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(39..286)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     252..270
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(336..356)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cccctcgagg tcgacggtat cgataagctt gatcggccgc aaattaaagc cttcgagcgt
       61 cccaaaacct tctcaagcaa ggttttcagt ataatgttac atgcgtacac gcgtctgtac
      121 agaaaaaaaa gaaaaatttg aaatataaat aacgttctta atactaacat aactataaaa
      181 aaataaatag ggacctagac ttcaggttgt ctaactcctt ccttttcggt tagagcggat
      241 gtggggggag ggcgtgaatg taagcgtgac ataactaatt acatgactcg agctatttgt
      301 atagttcatc catgccatgt gtaatcccag cagctgttac aaactcaaga aggaccatgt
      361 ggtctctctt ttcgttggga tctttcgaaa gggcagattg tgtggacagg taatggttgt
      421 ctggtaaaag gacagggcca tcgccaattg gagtattttg ttgataatgg tctgctagtt
      481 gaacgcttcc atcttcaatg ttgtgtctaa ttttgaaatg agctttgatt ccattctttt
      541 gtttgtctgc agtgatgtat acattgtgtg aaatatagtt gtattccaat ttgtgtccaa
      601 gaatgtttcc atcttcttta aaannnntnn ntttttaact cgatnnntnn aacnnnnnna
      661 tcacctttna aactgtgact tcagcacgtg tcttgtagtt cccgtcnnct ttnagaaana
      721 tnnngttcnt tntcgtgnac nnnanctncg gncntngcac tcttnaaaaa gtcatgccgt
      781 ttcatntgat ctggtatctt gaaagcattg annccccnag tc
//
