LOCUS       sequence-221569-       15436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral expression vector containing N terminal FLAG/HA
            tagged human PALI1 coding sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15436)
  AUTHORS   Conway E, Jerman E, Healy E, Ito S, Holoch D, Oliviero G, Deevy O, 
            Glancy E, Fitzpatrick DJ, Mucha M, Watson A, Rice AM, Chammas P, 
            Huang C, Pratt-Kelly I, Koseki Y, Nakayama M, Ishikura T, Streubel 
            G, Wynne K, Hokamp K, McLysaght A, Ciferri C, Di Croce L, Cagney G, 
            Margueron R, Koseki H, Bracken AP
  TITLE     A Family of Vertebrate-Specific Polycombs Encoded by the LCOR/LCORL 
            Genes Balance PRC2 Subtype Activities.
  JOURNAL   Mol Cell. 2018 May 3;70(3):408-421.e8. doi: 
            10.1016/j.molcel.2018.03.005. Epub 2018 Apr 5.
  PUBMED    29628311
REFERENCE   2  (bases 1 to 15436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.03.005"; journalName: "Mol Cell"; date: 
            "2018-05-3- 3"; volume: "70"; issue: "3"; pages: "408-421.e8"
COMMENT     Sequence Label: pLENTI EF1alpha FLAG/HA-PALI1
FEATURES             Location/Qualifiers
     source          1..15436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    95..557
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(151..168)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     378..397
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             590..1189
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(590..609)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1086..1106
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1467..2055
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1520..1540)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1938..1949)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2058..2074)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2059..2075)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2580..2760
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2786..2803)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2792..3016
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3062..3490
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3149..3168)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3359..3380
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3499..3519)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3504..3833
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3684..3819
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3746..3765
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3881..3928
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3947..4321
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4451..4572
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4488..4507)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4542..4561
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4621..4637)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4621..4637)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4634..4656)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4645..4661
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4669..4675,4676..4693,4694..4699))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4714..4735
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4852..4869)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5023..5611)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5103..5122)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5782..6573,6574..6642))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6405..6424
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6643..6747)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6822..6841)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7013..7392
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7394..7592
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7547..7567
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7610..7790
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7837..7962
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8455..8688
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8873..8917
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9215..9332
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9379..10557
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9610..10548
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     10505..10525
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             10587..10634
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             10635..10661
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    10668..10692
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(15394..15418)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
       61 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
      121 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
      181 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
      241 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
      301 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
      361 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
      421 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
      481 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
      541 gaaaaacacg atgataagct tgccacaacc cacaaggaga cgaccttcca tgaccgagta
      601 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc
      661 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga
      721 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
      781 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
      841 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
      901 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
      961 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     1021 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     1081 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     1141 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca
     1201 cgacccgcag cgcccgaccg aaaggagcgc acgaccccat ggctccgacc gaagccaccc
     1261 ggggcggccc cgccgacccc gcacccgccc ccgaggccca ccgactctag tcgagggctg
     1321 cagcgctgca gaggccgagt gcagaactgc tccaaaggga cctcaaggct ttccgaggga
     1381 cactaggctg actccatcga gccagtgtag agataagctt atcgattagt ccaatttgtt
     1441 aaagacagga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     1501 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     1561 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     1621 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     1681 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     1741 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     1801 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     1861 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     1921 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     1981 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     2041 gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca
     2101 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     2161 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     2221 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     2281 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     2341 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
     2401 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
     2461 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
     2521 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg
     2581 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     2641 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     2701 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     2761 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     2821 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     2881 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     2941 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     3001 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     3061 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     3121 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     3181 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     3241 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     3301 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     3361 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     3421 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     3481 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     3541 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     3601 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3661 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     3721 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     3781 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     3841 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
     3901 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac
     3961 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
     4021 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
     4081 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
     4141 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
     4201 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
     4261 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
     4321 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     4381 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     4441 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     4501 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     4561 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     4621 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     4681 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     4741 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     4801 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     4861 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4921 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4981 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5041 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5101 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5161 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     5221 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5281 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5341 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5401 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5461 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5521 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     5581 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     5641 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     5701 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     5761 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     5821 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     5881 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     5941 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     6001 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     6061 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     6121 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     6181 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     6241 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     6301 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     6361 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     6421 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     6481 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     6541 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     6601 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     6661 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     6721 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     6781 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
     6841 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     6901 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
     6961 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
     7021 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     7081 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     7141 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     7201 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     7261 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     7321 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     7381 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     7441 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     7501 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     7561 aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg
     7621 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7681 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7741 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     7801 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     7861 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     7921 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     7981 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     8041 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     8101 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     8161 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     8221 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     8281 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     8341 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     8401 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     8461 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     8521 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     8581 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     8641 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     8701 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     8761 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     8821 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     8881 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     8941 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     9001 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     9061 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     9121 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
     9181 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     9241 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     9301 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     9361 cagtttggtt aattaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     9421 gtccccgaga agttgtgggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
     9481 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     9541 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     9601 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
     9661 ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt
     9721 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     9781 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     9841 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     9901 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     9961 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
    10021 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
    10081 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
    10141 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
    10201 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
    10261 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
    10321 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
    10381 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
    10441 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
    10501 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga
    10561 attcggccat tacggccgat atcatggact acaaggacga cgatgacaag gactacaagg
    10621 acgacgatga caagtaccca tacgatgttc cagattacgc tggatctaca agtttgtaca
    10681 aaaaagcagg ctccgaattc gcccttatgc agcgaatgat ccaacaattt gctgctgaat
    10741 atacctcaaa aaatagctct actcaggacc ccagccagcc caatagcaca aagaaccaaa
    10801 gcctgccgaa agcatctcca gtcaccacct ctcccacggc tgcaactact cagaaccctg
    10861 tgctcagcaa acttctcatg gctgaccaag actcacctct ggaccttact gtcagaaagt
    10921 ctcagtcaga acctagcgaa caagacggtg tacttgatct gtccactaag aaaagtccat
    10981 gtgctggcag cacttccctg agccactctc caggctgctc cagtactcaa gggaacggtg
    11041 agaactcaac agaggcaaaa gcagtagatt ctaacaatca gtcgaagtcc ccactggaga
    11101 aatttatggt caaactgtgt actcatcatc aaaagcaatt cattcgtgtt ctgaacgacc
    11161 tgtacactga atctcaacca ggcactgagg acctgcagcc ttctgattcg ggagcaatgg
    11221 atgtatccac ttgcaatgct ggctgtgccc agctcagcac caaacataag gaaaaagatg
    11281 ctctgtgtct cgatatgaag tcttctgctt ctgtagattt gttcgtagac tcgtcagact
    11341 ctcacagccc tctacacttg acggaacaga ccccgaagaa gcctcctcct gagataaacc
    11401 ctgtagatgg aagagagaat gccttgactg ttgtccagaa agattcctct gaacttccaa
    11461 ccactaaatc gaattctatt aatagcagtt cagtggatag tttcactccg ggatacctca
    11521 ctgcatctaa ttgttcctca gtgaacttcc accacatccc taaaatcttg gaggggcaga
    11581 ccactggaca agagcaagac acaaatgtga acatatgtga ggatggtaaa gaccatatgc
    11641 agagttcagc tttagtagaa agtctaatta cagtaaaaat ggcagctgag aatagtgagg
    11701 aaggcaatac ctgtattatt cctcaaagaa atttgttcaa agctttatca gaagaggctt
    11761 ggaactcagg gtttatgggg aactcatcta gaactgctga caaagagaat actttacagt
    11821 gtccaaaaac acctttgcgc caggatttag aggcaaatga acaagatgca aggccaaagc
    11881 aagagaacca tcttcactct ctgggaagaa ataaggtggg ttaccattta catcccagtg
    11941 ataagggcca gtttgatcat tccaaagatg gttggttagg ccccggccct atgccagctg
    12001 tacacaaagc ggcaaatgga cactcaagaa ccaagatgat atcaacctcc atcaagacag
    12061 ctcggaaaag taaaagggca tcagggctga ggataaatga ttatgataac cagtgtgatg
    12121 ttgtttatat cagtcaacca ataacagaat gccactttga gaatcaaaaa tcaatattat
    12181 cttctcggaa aacagccaga aagagtactc gaggatactt tttcaatggt gactgttgtg
    12241 agctgccaac tgttcgtaca ctggccagaa atttacactc ccaggaaaaa gcaagctgct
    12301 cagcattggc atcagaggca gttttcactc ctaagcagac ccttacaatt ccagccccta
    12361 gacatacagt agatgtgcag cttcccagag aagacaaccc tgaagaacct agcaaggaaa
    12421 tcacctctca cgaggaagga ggtggagacg tttcacctcg aaaagaacct caagagcctg
    12481 aggtttgccc cacaaagatt aagccgaacc tgagcagctc ccctaggtca gaggaaacga
    12541 cagcctccag cctggtgtgg cctctccctg ctcaccttcc tgaagaggac ctgccagaag
    12601 gtggctccac agtctcagct cccacagcaa gtgggatgtc ttctcctgaa cacaaccaac
    12661 caccagttgc actgttggat acggaggaga tgagtgtacc ccaggactgt cacctccttc
    12721 cctccactga aagcttttcc gggggagtca gtgaagatgt catttctagg cctcattctc
    12781 ctcctgaaat agtcagtaga gaagaaagtc ctcagtgctc agaaaatcag agttccccaa
    12841 tgggcttgga gccccccatg agtctgggaa aggctgagga caaccaaagc atcagtgctg
    12901 aggttgagtc tggagacacc caggagctaa atgtcgaccc actcttgaag gaaagcagca
    12961 cttttactga tgaaaacccc agtgaaactg aggaaagtga ggcagcaggt ggtataggaa
    13021 aattagaggg agaggacggt gatgtaaaat gcctgtcaga aaaagacacg tatgatacaa
    13081 gcattgactc actcgaagag aatttggaca agaagaaaaa aggtaaaaaa ttccctgagg
    13141 cctctgatag gtgcctaaga agtcaacttt cggattcttc ctctgctgac agatgcctaa
    13201 gaaatcagag ttcagattct tcctcagctt gtcttgaaat caaagttcct aaaaatccta
    13261 gtgcaaaacg ttcaaaaaaa gaagggcacc ctggtgggac aacacctaag ggccttctac
    13321 ctgacagttt ccacacggaa actctggagg acacagaaaa gccaagtgtc aatgaacgcc
    13381 cctctgagaa agatgctgag caggagggcg aaggcggggg gatcatcacc aggcagactt
    13441 tgaaaaacat gctggacaaa gaagtcaagg agttacgagg agagattttc cccagcaggg
    13501 accccataac cacagctgga cagccactgc ctggagagag attggaaatc tatgttcagt
    13561 ctaaaatgga tgagaagaat gctcatatcc cctcagaaag tattgcttgt aagagggacc
    13621 cagaacaggc aaaagaagag ccagggcata ttcccacaca gcatgtggag gaggctgtga
    13681 atgaggtaga caacgaaaac acccagcaga aagatgatga gagtgatgcc ccatgcagct
    13741 ctcttgggtt gtcgagtagt ggaagtggtg atgctgctag ggcaccaaaa tcggtgccaa
    13801 ggcctaaaag attgacctct tcaacctaca acctaagaca cgctcattct ctgggctcct
    13861 tggatgcttc aaaagtgact tcagaaaagg aagctgcaca agtaaacccc ataatgccaa
    13921 aggaaaatgg agcttcagag agtggagacc ccctagatga ggacgatgtt gacaccgtgg
    13981 tagatgaaca gccaaagttt atggaatggt gtgctgagga ggagaaccaa gagctcatcg
    14041 ccaacttcaa tgcccagtac atgaaagttc agaagggctg gatccagttg gagaaagaag
    14101 gacagccaac accaagagca aggaacaaat cagataaact gaaagagatt tggaaaagca
    14161 agaaaaggtc acggaaatgt aggagttcat tggagagtca gaagtgttct cctgttcaga
    14221 tgctctttat gacaaacttt aaattatcta atgtttgtaa atggttctta gagacaactg
    14281 aaacccggtc tctagtcatt gtgaagaagc tcaatactcg ccttccagga gacgttcccc
    14341 ctgtcaagca tcctcttcag aaatacgctc cttccagcct atatcccagt tcactacagg
    14401 ctgagcgctt gaaaaagcac ttgaagaaat ttcctggagc tacccctgct aagaataatt
    14461 ggaaaatgca gaagctctgg gccaaatttc gagagaatcc tgatcaagtg gagccagaag
    14521 atggcagtga tgtcagcccc ggccctaatt ctgaagacag catagaggaa gtcaaggaag
    14581 atagaaacag tcatcctcca gcaaacctgc ccactccagc cagtacccgg attcttagaa
    14641 aatattccaa tattcgagga aagctcagag cccagcaacg tttaatcaag aatgagaaaa
    14701 tggaatgccc agatgctctg gctgtggaaa gtaagccaag tcgtaagagc gtatgcatca
    14761 accctctgat gtcccccaag cttgccctgc aagtggatgc agatgggttt cctgttaagc
    14821 ccaagagtac tgaaggaatg aagggaagga aggggaagca ggtgtctgaa atcttgccta
    14881 aagcagaagt tcagagtaaa cgcaagagaa cagaaggcag cagccctcca gatagtaaga
    14941 acaaggggcc tacggtgaaa gccagcaaag aaaagcatgc tgatggagcc accaaaaccc
    15001 ctgctgccaa gaggccagct gcaagggaca gaagcagcca accccccaaa aagacgtctt
    15061 tgaaagagaa taaagtgaag atccctaaaa agtccgctgg gaagagctgc cctccctcca
    15121 ggaaagaaaa agagaataca aacaaaaggc cttcccagtc tattgcctcg gaaacactga
    15181 cgaaacctgc aaaacagaag ggggccggtg aatcctcttc aaggcctcag aaagccacga
    15241 ataggaagca gagtagtgga aagactcggg ccagaccctc aacgaaaacc ccagagagca
    15301 gtgcagctca gagaaagcga aagctgaagg caaagctgga ctgttcgcac agcaaacgga
    15361 ggcggctgga tgcaaagtga aagggcgaat tcgacccagc tttcttgtac aaagtggtag
    15421 atccggaatt cccccc
//
