LOCUS       sequence-221606-       14464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect baculovirus expression vector containing N terminal FLAG/His 
            tagged human PALI1 coding sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14464)
  AUTHORS   Conway E, Jerman E, Healy E, Ito S, Holoch D, Oliviero G, Deevy O, 
            Glancy E, Fitzpatrick DJ, Mucha M, Watson A, Rice AM, Chammas P, 
            Huang C, Pratt-Kelly I, Koseki Y, Nakayama M, Ishikura T, Streubel 
            G, Wynne K, Hokamp K, McLysaght A, Ciferri C, Di Croce L, Cagney G, 
            Margueron R, Koseki H, Bracken AP
  TITLE     A Family of Vertebrate-Specific Polycombs Encoded by the LCOR/LCORL 
            Genes Balance PRC2 Subtype Activities.
  JOURNAL   Mol Cell. 2018 May 3;70(3):408-421.e8. doi: 
            10.1016/j.molcel.2018.03.005. Epub 2018 Apr 5.
  PUBMED    29628311
REFERENCE   2  (bases 1 to 14464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.03.005"; journalName: "Mol Cell"; date: 
            "2018-05-3- 3"; volume: "70"; issue: "3"; pages: "408-421.e8"
COMMENT     Sequence Label: pVL-FLAG/His PALI1
FEATURES             Location/Qualifiers
     source          1..14464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             152..175
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             176..199
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     protein_bind    209..233
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4935..4959)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     5479..6177
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           5560..5932
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(5589..6177)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     CDS             complement(6611..6628)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7845..7861)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7845..7861)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7858..7880)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7869..7885
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7893..7899,7900..7917,7918..7923))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7938..7959
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8076..8093)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8247..8835)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8327..8346)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9006..9797,9798..9866))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9629..9648
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9867..9971)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10039..10057
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10095..10117)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10217..10236
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10430..10452
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10444..10461
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10445..10461
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_recomb     13634..14461
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             13634..13758
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(13796..14401)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atcaccatgg actacaaaga cgattacaag gatgacgatg acaagcatca
      181 tcatcatcat catcatcatg ggggatctac aagtttgtac aaaaaagcag gctccgaatt
      241 cgcccttatg cagcgaatga tccaacaatt tgctgctgaa tatacctcaa aaaatagctc
      301 tactcaggac cccagccagc ccaatagcac aaagaaccaa agcctgccga aagcatctcc
      361 agtcaccacc tctcccacgg ctgcaactac tcagaaccct gtgctcagca aacttctcat
      421 ggctgaccaa gactcacctc tggaccttac tgtcagaaag tctcagtcag aacctagcga
      481 acaagacggt gtacttgatc tgtccactaa gaaaagtcca tgtgctggca gcacttccct
      541 gagccactct ccaggctgct ccagtactca agggaacggt gagaactcaa cagaggcaaa
      601 agcagtagat tctaacaatc agtcgaagtc cccactggag aaatttatgg tcaaactgtg
      661 tactcatcat caaaagcaat tcattcgtgt tctgaacgac ctgtacactg aatctcaacc
      721 aggcactgag gacctgcagc cttctgattc gggagcaatg gatgtatcca cttgcaatgc
      781 tggctgtgcc cagctcagca ccaaacataa ggaaaaagat gctctgtgtc tcgatatgaa
      841 gtcttctgct tctgtagatt tgttcgtaga ctcgtcagac tctcacagcc ctctacactt
      901 gacggaacag accccgaaga agcctcctcc tgagataaac cctgtagatg gaagagagaa
      961 tgccttgact gttgtccaga aagattcctc tgaacttcca accactaaat cgaattctat
     1021 taatagcagt tcagtggata gtttcactcc gggatacctc actgcatcta attgttcctc
     1081 agtgaacttc caccacatcc ctaaaatctt ggaggggcag accactggac aagagcaaga
     1141 cacaaatgtg aacatatgtg aggatggtaa agaccatatg cagagttcag ctttagtaga
     1201 aagtctaatt acagtaaaaa tggcagctga gaatagtgag gaaggcaata cctgtattat
     1261 tcctcaaaga aatttgttca aagctttatc agaagaggct tggaactcag ggtttatggg
     1321 gaactcatct agaactgctg acaaagagaa tactttacag tgtccaaaaa cacctttgcg
     1381 ccaggattta gaggcaaatg aacaagatgc aaggccaaag caagagaacc atcttcactc
     1441 tctgggaaga aataaggtgg gttaccattt acatcccagt gataagggcc agtttgatca
     1501 ttccaaagat ggttggttag gccccggccc tatgccagct gtacacaaag cggcaaatgg
     1561 acactcaaga accaagatga tatcaacctc catcaagaca gctcggaaaa gtaaaagggc
     1621 atcagggctg aggataaatg attatgataa ccagtgtgat gttgtttata tcagtcaacc
     1681 aataacagaa tgccactttg agaatcaaaa atcaatatta tcttctcgga aaacagccag
     1741 aaagagtact cgaggatact ttttcaatgg tgactgttgt gagctgccaa ctgttcgtac
     1801 actggccaga aatttacact cccaggaaaa agcaagctgc tcagcattgg catcagaggc
     1861 agttttcact cctaagcaga cccttacaat tccagcccct agacatacag tagatgtgca
     1921 gcttcccaga gaagacaacc ctgaagaacc tagcaaggaa atcacctctc acgaggaagg
     1981 aggtggagac gtttcacctc gaaaagaacc tcaagagcct gaggtttgcc ccacaaagat
     2041 taagccgaac ctgagcagct cccctaggtc agaggaaacg acagcctcca gcctggtgtg
     2101 gcctctccct gctcaccttc ctgaagagga cctgccagaa ggtggctcca cagtctcagc
     2161 tcccacagca agtgggatgt cttctcctga acacaaccaa ccaccagttg cactgttgga
     2221 tacggaggag atgagtgtac cccaggactg tcacctcctt ccctccactg aaagcttttc
     2281 cgggggagtc agtgaagatg tcatttctag gcctcattct cctcctgaaa tagtcagtag
     2341 agaagaaagt cctcagtgct cagaaaatca gagttcccca atgggcttgg agccccccat
     2401 gagtctggga aaggctgagg acaaccaaag catcagtgct gaggttgagt ctggagacac
     2461 ccaggagcta aatgtcgacc cactcttgaa ggaaagcagc acttttactg atgaaaaccc
     2521 cagtgaaact gaggaaagtg aggcagcagg tggtatagga aaattagagg gagaggacgg
     2581 tgatgtaaaa tgcctgtcag aaaaagacac gtatgataca agcattgact cactcgaaga
     2641 gaatttggac aagaagaaaa aaggtaaaaa attccctgag gcctctgata ggtgcctaag
     2701 aagtcaactt tcggattctt cctctgctga cagatgccta agaaatcaga gttcagattc
     2761 ttcctcagct tgtcttgaaa tcaaagttcc taaaaatcct agtgcaaaac gttcaaaaaa
     2821 agaagggcac cctggtggga caacacctaa gggccttcta cctgacagtt tccacacgga
     2881 aactctggag gacacagaaa agccaagtgt caatgaacgc ccctctgaga aagatgctga
     2941 gcaggagggc gaaggcgggg ggatcatcac caggcagact ttgaaaaaca tgctggacaa
     3001 agaagtcaag gagttacgag gagagatttt ccccagcagg gaccccataa ccacagctgg
     3061 acagccactg cctggagaga gattggaaat ctatgttcag tctaaaatgg atgagaagaa
     3121 tgctcatatc ccctcagaaa gtattgcttg taagagggac ccagaacagg caaaagaaga
     3181 gccagggcat attcccacac agcatgtgga ggaggctgtg aatgaggtag acaacgaaaa
     3241 cacccagcag aaagatgatg agagtgatgc cccatgcagc tctcttgggt tgtcgagtag
     3301 tggaagtggt gatgctgcta gggcaccaaa atcggtgcca aggcctaaaa gattgacctc
     3361 ttcaacctac aacctaagac acgctcattc tctgggctcc ttggatgctt caaaagtgac
     3421 ttcagaaaag gaagctgcac aagtaaaccc cataatgcca aaggaaaatg gagcttcaga
     3481 gagtggagac cccctagatg aggacgatgt tgacaccgtg gtagatgaac agccaaagtt
     3541 tatggaatgg tgtgctgagg aggagaacca agagctcatc gccaacttca atgcccagta
     3601 catgaaagtt cagaagggct ggatccagtt ggagaaagaa ggacagccaa caccaagagc
     3661 aaggaacaaa tcagataaac tgaaagagat ttggaaaagc aagaaaaggt cacggaaatg
     3721 taggagttca ttggagagtc agaagtgttc tcctgttcag atgctcttta tgacaaactt
     3781 taaattatct aatgtttgta aatggttctt agagacaact gaaacccggt ctctagtcat
     3841 tgtgaagaag ctcaatactc gccttccagg agacgttccc cctgtcaagc atcctcttca
     3901 gaaatacgct ccttccagcc tatatcccag ttcactacag gctgagcgct tgaaaaagca
     3961 cttgaagaaa tttcctggag ctacccctgc taagaataat tggaaaatgc agaagctctg
     4021 ggccaaattt cgagagaatc ctgatcaagt ggagccagaa gatggcagtg atgtcagccc
     4081 cggccctaat tctgaagaca gcatagagga agtcaaggaa gatagaaaca gtcatcctcc
     4141 agcaaacctg cccactccag ccagtacccg gattcttaga aaatattcca atattcgagg
     4201 aaagctcaga gcccagcaac gtttaatcaa gaatgagaaa atggaatgcc cagatgctct
     4261 ggctgtggaa agtaagccaa gtcgtaagag cgtatgcatc aaccctctga tgtcccccaa
     4321 gcttgccctg caagtggatg cagatgggtt tcctgttaag cccaagagta ctgaaggaat
     4381 gaagggaagg aaggggaagc aggtgtctga aatcttgcct aaagcagaag ttcagagtaa
     4441 acgcaagaga acagaaggca gcagccctcc agatagtaag aacaaggggc ctacggtgaa
     4501 agccagcaaa gaaaagcatg ctgatggagc caccaaaacc cctgctgcca agaggccagc
     4561 tgcaagggac agaagcagcc aaccccccaa aaagacgtct ttgaaagaga ataaagtgaa
     4621 gatccctaaa aagtccgctg ggaagagctg ccctccctcc aggaaagaaa aagagaatac
     4681 aaacaaaagg ccttcccagt ctattgcctc ggaaacactg acgaaacctg caaaacagaa
     4741 gggggccggt gaatcctctt caaggcctca gaaagccacg aataggaagc agagtagtgg
     4801 aaagactcgg gccagaccct caacgaaaac cccagagagc agtgcagctc agagaaagcg
     4861 aaagctgaag gcaaagctgg actgttcgca cagcaaacgg aggcggctgg atgcaaagtg
     4921 aaagggcgaa ttcgacccag ctttcttgta caaagtggta gatccgttta aacaattccg
     4981 gagcggccgc tgcagatctg atcctttcct gggacccggc aagaaccaaa aactcactct
     5041 cttcaaggaa atccgtaatg ttaaacccga cacgatgaag cttgtcgttg gatggaaagg
     5101 aaaagagttc tacagggaaa cttggacccg cttcatggaa gacagcttcc ccattgttaa
     5161 cgaccaagaa gtgatggatg ttttccttgt tgtcaacatg cgtcccacta gacccaaccg
     5221 ttgttacaaa ttcctggccc aacacgctct gcgttgcgac cccgactatg tacctcatga
     5281 cgtgattagg atcgtcgagc cttcatgggt gggcagcaac aacgagtacc gcatcagcct
     5341 ggctaagaag ggcggcggct gcccaataat gaaccttcac tctgagtaca ccaactcgtt
     5401 cgaacagttc atcgatcgtg tcatctggga gaacttctac aagcccatcg tttacatcgg
     5461 taccgactct gctgaagagg aggaaattct ccttgaagtt tccctggtgt aaaggagttt
     5521 gcaccagacg cacctctgtt cactggtccg gcgtattaaa acacgataca ttgttattag
     5581 tacatttatt aagcgctaga ttctgtgcgt tgttgattta cagacaattg ttgtacgtat
     5641 tttaataatt cattaaattt ataatcttta gggtggtatg ttagagcgaa aatcaaatga
     5701 ttttcagcgt ctttatatct gaatttaaat attaaatcct caatagattt gtaaaatagg
     5761 tttcgattag tttcaaacaa gggttgtttt tccgaaccga tggctggact atctaatgga
     5821 ttttcgctca acgccacaaa acttgccaaa tcttgtagca gcaatctagc tttgtcgata
     5881 ttcgtttgtg ttttgttttg taataaaggt tcgacgtcgt tcaaaatatt atgcgctttt
     5941 gtatttcttt catcactgtc gttagtgtac aattgactcg acgtaaacac gttaaataaa
     6001 gcttggacat atttaacatc gggcgtgtta gctttattag gccgattatc gtcgtcgtcc
     6061 caaccctcgt cgttagaagt tgcttccgaa gacgattttg ccatagccac acgacgccta
     6121 ttaattgtgt cggctaacac gtccgcgatc aaatttgtag ttgagctttt tggaattatt
     6181 tctgattgcg ggcgtttttg ggcgggtttc aatctaactg tgcccgattt taattcagac
     6241 aacacgttag aaagcgatgg tgcaggcggt ggtaacattt cagacggcaa atctactaat
     6301 ggcggcggtg gtggagctga tgataaatct accatcggtg gaggcgcagg cggggctggc
     6361 ggcggaggcg gaggcggagg tggtggcggt gatgcagacg gcggtttagg ctcaaatgtc
     6421 tctttaggca acacagtcgg cacctcaact attgtactgg tttcgggcgc cgtttttggt
     6481 ttgaccggtc tgagacgagt gcgatttttt tcgtttctaa tagcttccaa caattgttgt
     6541 ctgtcgtcta aaggtgcagc gggttgaggt tccgtcggca ttggtggagc gggcggcaat
     6601 tcagacatcg atggtggtgg tggtggtgga ggcgctggaa tgttaggcac gggagaaggt
     6661 ggtggcggcg gtgccgccgg tataatttgt tctggtttag tttgttcgcg cacgattgtg
     6721 ggcaccggcg caggcgccgc tggctgcaca acggaaggtc gtctgcttcg aggcagcgct
     6781 tggggtggtg gcaattcaat attataattg gaatacaaat cgtaaaaatc tgctataagc
     6841 attgtaattt cgctatcgtt taccgtgccg atatttaaca accgctcaat gtaagcaatt
     6901 gtattgtaaa gagattgtct caagctcgca cgccgataac aagccttttc atttttacta
     6961 cagcattgta gtggcgagac acttcgctgt cgtcgacgta catgtatgct ttgttgtcaa
     7021 aaacgtcgtt ggcaagcttt aaaatattta aaagaacatc tctgttcagc accactgtgt
     7081 tgtcgtaaat gttgtttttg ataatttgcg cttccgcagt atcgacacgt tcaaaaaatt
     7141 gatgcgcatc aattttgttg ttcctattat tgaataaata agattgtaca gattcatatc
     7201 tacgattcgt catggccacc acaaatgcta cgctgcaaac gctggtacaa ttttacgaaa
     7261 actgcaaaaa cgtcaaaact cggtataaaa taatcaacgg gcgctttggc aaaatatcta
     7321 ttttatcgca caagcccact agcaaattgt atttgcagaa aacaatttcg gcgcacaatt
     7381 ttaacgctga cgaaataaaa gttcaccagt taatgagcga ccacccaaat tttataaaaa
     7441 tctattttaa tcacggttcc atcaacaacc aagtgatcgt gatggactac attgactgtc
     7501 ccgatttatt tgaaacacta caaattaaag gcgagctttc gtaccaactt gttagcaata
     7561 ttattagaca gctgtgtgaa gcgctcaacg atttgcacaa gcacaatttc atacacaacg
     7621 acataaaact cgaaaatgtc ttatatttcg aagcacttga tcgcgtgtat gtttgcgatt
     7681 acggattgtg caaacacgaa aactcactta gcgtgcacga cggcacgttg gagtatttta
     7741 gtccggaaaa aattcgacac cacaactatg cacgttcgtt tgactggtac gccgtcggcg
     7801 tgttaacata caagttgcta accggcggcc aattcgtaat catggtcata gctgtttcct
     7861 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7921 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7981 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8041 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8101 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8161 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8221 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8281 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8341 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8401 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8461 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8521 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8581 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8641 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8701 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8761 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8821 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8881 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8941 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9001 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9061 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9121 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9181 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9241 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9301 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9361 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9421 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9481 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9541 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9601 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9661 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9721 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9781 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9841 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9901 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9961 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
    10021 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
    10081 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
    10141 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
    10201 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
    10261 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
    10321 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
    10381 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
    10441 cgttgtaaaa cgacggccag tgccaagctt ggctcgtaaa gcgagttgaa ggatcatatt
    10501 tagttgcgtt tatgagataa gattgaaagc acgtgtaaaa tgtttcccgc gcgttggcac
    10561 aactatttac aatgcggcca agttataaaa gattctaatc tgatatgttt taaaacacct
    10621 ttgcggcccg agttgtttgc gtacgtgact agcgaagaag atatgtggac cgcagaacag
    10681 atagtaaaac aaaaccctag tattggagca ataatcgatt taaccaacac gtctaaatat
    10741 tatgatggtg tgcatttttt gcgggcgggc ctgttataca aaaaaattca agtacctggc
    10801 cagactttgc cgcctgaaag catagttcaa gaatttattg acacggtaaa agaatttaca
    10861 gaaaagtgtc ccggcatgtt ggtgggcgtg cactgcacac acggtattaa tcgcaccggt
    10921 tacatggtgt gcagatattt aatgcacacc ctgggtattg cgccgcagga agccatagat
    10981 agattcgaaa aagccagagg tcacaaaatt gaaagacaaa attacgttca agatttatta
    11041 atttaattaa tattatttgc attctttaac aaatacttta tcctattttc aaattgttgc
    11101 gcttcttcca gcgaaccaaa actatgcttc gcttgctccg tttagcttgt agccgatcag
    11161 tggcgttgtt ccaatcgacg gtaggattag gccggatatt ctccaccaca atgttggcaa
    11221 cgttgatgtt acgtttatgc ttttggtttt ccacgtacgt cttttggccg gtaatagccg
    11281 taaacgtagt gccgtcgcgc gtcacgcaca acaccggatg tttgcgcttg tccgcggggt
    11341 attgaaccgc gcgatccgac aaatccacca ctttggcaac taaatcggtg acctgcgcgt
    11401 cttttttctg cattatttcg tctttctttt gcatggtttc ctggaagccg gtgtacatgc
    11461 ggtttagatc agtcatgacg cgcgtgacct gcaaatcttt ggcctcgatc tgcttgtcct
    11521 tgatggcaac gatgcgttca ataaactctt gttttttaac aagttcctcg gttttttgcg
    11581 ccaccaccgc ttgcagcgcg tttgtgtgct cggtgaatgt cgcaatcagc ttagtcacca
    11641 actgtttgct ctcctcctcc cgttgtttga tcgcgggatc gtacttgccg gtgcagagca
    11701 cttgaggaat tacttcttct aaaagccatt cttgtaattc tatggcgtaa ggcaatttgg
    11761 acttcataat cagctgaatc acgccggatt tagtaatgag cactgtatgc ggctgcaaat
    11821 acagcgggtc gccccttttc acgacgctgt tagaggtagg gcccccattt tggatggtct
    11881 gctcaaataa cgatttgtat ttattgtcta catgaacacg tatagcttta tcacaaactg
    11941 tatattttaa actgttagcg acgtccttgg ccacgaaccg gacctgttgg tcgcgctcta
    12001 gcacgtaccg caggttgaac gtatcttctc caaatttaaa ttctccaatt ttaacgcgag
    12061 ccattttgat acacgtgtgt cgattttgca acaactattg ttttttaacg caaactaaac
    12121 ttattgtggt aagcaataat taaatatggg ggaacatgcg ccgctacaac actcgtcgtt
    12181 atgaacgcag acggcgccgg tctcggcgca agcggctaaa acgtgttgcg cgttcaacgc
    12241 ggcaaacatc gcaaaagcca atagtacagt tttgatttgc atattaacgg cgatttttta
    12301 aattatctta tttaataaat agttatgacg cctacaactc cccgcccgcg ttgactcgct
    12361 gcacctcgag cagttcgttg acgccttcct ccgtgtggcc gaacacgtcg agcgggtggt
    12421 cgatgaccag cggcgtgccg cacgcgacgc acaagtatct gtacaccgaa tgatcgtcgg
    12481 gcgaaggcac gtcggcctcc aagtggcaat attggcaaat tcgaaaatat atacagttgg
    12541 gttgtttgcg catatctatc gtggcgttgg gcatgtacgt ccgaacgttg atttgcatgc
    12601 aagccgaaat taaatcattg cgattagtgc gattaaaacg ttgtacatcc tcgcttttaa
    12661 tcatgccgtc gattaaatcg cgcaatcgag tcaagtgatc aaagtgtgga ataatgtttt
    12721 ctttgtattc ccgagtcaag cgcagcgcgt attttaacaa actagccatc ttgtaagtta
    12781 gtttcattta atgcaacttt atccaataat atattatgta tcgcacgtca agaattaaca
    12841 atgcgcccgt tgtcgcatct caacacgact atgatagaga tcaaataaag cgcgaattaa
    12901 atagcttgcg acgcaacgtg cacgatctgt gcacgcgttc cggcacgagc tttgattgta
    12961 ataagttttt acgaagcgat gacatgaccc ccgtagtgac aacgatcacg cccaaaagaa
    13021 ctgccgacta caaaattacc gagtatgtcg gtgacgttaa aactattaag ccatccaatc
    13081 gaccgttagt cgaatcagga ccgctggtgc gagaagccgc gaagtatggc gaatgcatcg
    13141 tataacgtgt ggagtccgct cattagagcg tcatgtttag acaagaaagc tacatattta
    13201 attgatcccg atgattttat tgataaattg accctaactc catacacggt attctacaat
    13261 ggcggggttt tggtcaaaat ttccggactg cgattgtaca tgctgttaac ggctccgccc
    13321 actattaatg aaattaaaaa ttccaatttt aaaaaacgca gcaagagaaa catttgtatg
    13381 aaagaatgcg tagaaggaaa gaaaaatgtc gtcgacatgc tgaacaacaa gattaatatg
    13441 cctccgtgta taaaaaaaat attgaacgat ttgaaagaaa acaatgtacc gcgcggcggt
    13501 atgtacagga agaggtttat actaaactgt tacattgcaa acgtggtttc gtgtgccaag
    13561 tgtgaaaacc gatgtttaat caaggctctg acgcatttct acaaccacga ctccaagtgt
    13621 gtgggtgaag tcatgcatct tttaatcaaa tcccaagatg tgtataaacc accaaactgc
    13681 caaaaaatga aaactgtcga caagctctgt ccgtttgctg gcaactgcaa gggtctcaat
    13741 cctatttgta attattgaat aataaaacaa ttataaatgt caaatttgtt ttttattaac
    13801 gatacaaacc aaacgcaaca agaacatttg tagtattatc tataattgaa aacgcgtagt
    13861 tataatcgct gaggtaatat ttaaaatcat tttcaaatga ttcacagtta atttgcgaca
    13921 atataatttt attttcacat aaactagacg ccttgtcgtc ttcttcttcg tattccttct
    13981 ctttttcatt tttctcctca taaaaattaa catagttatt atcgtatcca tatatgtatc
    14041 tatcgtatag agtaaatttt ttgttgtcat aaatatatat gtctttttta atggggtgta
    14101 tagtaccgct gcgcatagtt tttctgtaat ttacaacagt gctattttct ggtagttctt
    14161 cggagtgtgt tgctttaatt attaaattta tataatcaat gaatttggga tcgtcggttt
    14221 tgtacaatat gttgccggca tagtacgcag cttcttctag ttcaattaca ccatttttta
    14281 gcagcaccgg attaacataa ctttccaaaa tgttgtacga accgttaaac aaaaacagtt
    14341 cacctccctt ttctatacta ttgtctgcga gcagttgttt gttgttaaaa ataacagcca
    14401 ttgtaatgag acgcacaaac taatatcaca aactggaaat gtctatcaat atatagttgc
    14461 tgat
//
