LOCUS       sequence-221608-       11099 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect baculovirus expression vector containing N terminal FLAG/His 
            tagged human LCOR coding sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11099)
  AUTHORS   Conway E, Jerman E, Healy E, Ito S, Holoch D, Oliviero G, Deevy O, 
            Glancy E, Fitzpatrick DJ, Mucha M, Watson A, Rice AM, Chammas P, 
            Huang C, Pratt-Kelly I, Koseki Y, Nakayama M, Ishikura T, Streubel 
            G, Wynne K, Hokamp K, McLysaght A, Ciferri C, Di Croce L, Cagney G, 
            Margueron R, Koseki H, Bracken AP
  TITLE     A Family of Vertebrate-Specific Polycombs Encoded by the LCOR/LCORL 
            Genes Balance PRC2 Subtype Activities.
  JOURNAL   Mol Cell. 2018 May 3;70(3):408-421.e8. doi: 
            10.1016/j.molcel.2018.03.005. Epub 2018 Apr 5.
  PUBMED    29628311
REFERENCE   2  (bases 1 to 11099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.03.005"; journalName: "Mol Cell"; date: 
            "2018-05-3- 3"; volume: "70"; issue: "3"; pages: "408-421.e8"
COMMENT     Sequence Label: pVL-FLAG/His LCOR
FEATURES             Location/Qualifiers
     source          1..11099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             152..175
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             176..199
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     protein_bind    209..233
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1563..1587)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     2107..2812
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           2195..2567
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(2224..2812)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     CDS             complement(3246..3263)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4480..4496)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4480..4496)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4493..4515)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4504..4520
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4528..4534,4535..4552,4553..4558))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4573..4594
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4711..4728)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4882..5470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4962..4981)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5641..6432,6433..6501))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6264..6283
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6502..6606)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6674..6692
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6730..6752)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6852..6871
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7065..7087
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7079..7096
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7080..7096
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_recomb     10269..11096
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             10269..10393
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(10431..11036)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atcaccatgg actacaaaga cgattacaag gatgacgatg acaagcatca
      181 tcatcatcat catcatcatg ggggatctac aagtttgtac aaaaaagcag gctccgaatt
      241 cgcccttatg cagcgaatga tccaacaatt tgctgctgaa tatacctcaa aaaatagctc
      301 tactcaggac cccagccagc ccaatagcac aaagaaccaa agcctgccga aagcatctcc
      361 agtcaccacc tctcccacgg ctgcaactac tcagaaccct gtgctcagca aacttctcat
      421 ggctgaccaa gactcacctc tggaccttac tgtcagaaag tctcagtcag aacctagcga
      481 acaagacggt gtacttgatc tgtccactaa gaaaagtcca tgtgctggca gcacttccct
      541 gagccactct ccaggctgct ccagtactca agggaacggg cgacctggga gacccagcca
      601 gtaccgccca gacggacttc ggagtggtga tggggtacct ccaagaagct tacaggatgg
      661 aaccagggaa ggttttggac actccacatc actcaaagtt ccactggctc gatccctgca
      721 gattagtgaa gaactactga gcagaaacca attgtccaca gctgccagcc ttgggccatc
      781 tggattacag aatcatggac aacacttaat attatccagg gaagcctctt gggcaaaacc
      841 acattacgag ttcaacctca gccgtatgaa gttcagggga aatggtgcac tcagcaacat
      901 cagtgacctt ccttttcttg cagaaaactc tgcctttcca aaaatggcac ttcaagcaaa
      961 acaagatgga aaaaaggatg tgagccattc atctcctgta gatttaaaga taccacaagt
     1021 tcgaggaatg gatctttctt gggagtctcg cactggtgat cagtacagct atagctcttt
     1081 ggtaatgggt tcacaaacgg agagcgcgct tagtaaaaaa ttaagggcta ttcttccaaa
     1141 acaaagtaga aaaagcatgt tagatgctgg acccgattct tggggctcag atgctgagca
     1201 gtctacctct ggacagccat atcccacatc ggatcaagaa ggagaccctg gctccaagca
     1261 gcctcggaag aaaagagggc gttacagaca gtacaacagt gagatactgg aggaagcaat
     1321 ctcagtggtt atgagtggaa aaatgagtgt ttccaaagct cagagtattt atgggattcc
     1381 ccacagtaca ctggagtaca aagtaaagga gaggctgggc actttgaaaa accctccaaa
     1441 gaaaaagatg aaattaatga ggtcggaggg gccagatgtt tctgtaaaga ttgaattaga
     1501 tccccaggga gaggcagcac aaagtgcaaa tgaatcaaaa aacgagtaga agggcgaatt
     1561 cgacccagct ttcttgtaca aagtggtaga tccgtttaaa caattccgga gcggccgctg
     1621 cagatctgat cctttcctgg gacccggcaa gaaccaaaaa ctcactctct tcaaggaaat
     1681 ccgtaatgtt aaacccgaca cgatgaagct tgtcgttgga tggaaaggaa aagagttcta
     1741 cagggaaact tggacccgct tcatggaaga cagcttcccc attgttaacg accaagaagt
     1801 gatggatgtt ttccttgttg tcaacatgcg tcccactaga cccaaccgtt gttacaaatt
     1861 cctggcccaa cacgctctgc gttgcgaccc cgactatgta cctcatgacg tgattaggat
     1921 cgtcgagcct tcatgggtgg gcagcaacaa cgagtaccgc atcagcctgg ctaagaaggg
     1981 cggcggctgc ccaataatga accttcactc tgagtacacc aactcgttcg aacagttcat
     2041 cgatcgtgtc atctgggaga acttctacaa gcccatcgtt tacatcggta ccgactctgc
     2101 tgaagaggag gaaattctcc ttgaagtttc cctggtgttc aaagtaaagg agtttgcacc
     2161 agacgcacct ctgttcactg gtccggcgta ttaaaacacg atacattgtt attagtacat
     2221 ttattaagcg ctagattctg tgcgttgttg atttacagac aattgttgta cgtattttaa
     2281 taattcatta aatttataat ctttagggtg gtatgttaga gcgaaaatca aatgattttc
     2341 agcgtcttta tatctgaatt taaatattaa atcctcaata gatttgtaaa ataggtttcg
     2401 attagtttca aacaagggtt gtttttccga accgatggct ggactatcta atggattttc
     2461 gctcaacgcc acaaaacttg ccaaatcttg tagcagcaat ctagctttgt cgatattcgt
     2521 ttgtgttttg ttttgtaata aaggttcgac gtcgttcaaa atattatgcg cttttgtatt
     2581 tctttcatca ctgtcgttag tgtacaattg actcgacgta aacacgttaa ataaagcttg
     2641 gacatattta acatcgggcg tgttagcttt attaggccga ttatcgtcgt cgtcccaacc
     2701 ctcgtcgtta gaagttgctt ccgaagacga ttttgccata gccacacgac gcctattaat
     2761 tgtgtcggct aacacgtccg cgatcaaatt tgtagttgag ctttttggaa ttatttctga
     2821 ttgcgggcgt ttttgggcgg gtttcaatct aactgtgccc gattttaatt cagacaacac
     2881 gttagaaagc gatggtgcag gcggtggtaa catttcagac ggcaaatcta ctaatggcgg
     2941 cggtggtgga gctgatgata aatctaccat cggtggaggc gcaggcgggg ctggcggcgg
     3001 aggcggaggc ggaggtggtg gcggtgatgc agacggcggt ttaggctcaa atgtctcttt
     3061 aggcaacaca gtcggcacct caactattgt actggtttcg ggcgccgttt ttggtttgac
     3121 cggtctgaga cgagtgcgat ttttttcgtt tctaatagct tccaacaatt gttgtctgtc
     3181 gtctaaaggt gcagcgggtt gaggttccgt cggcattggt ggagcgggcg gcaattcaga
     3241 catcgatggt ggtggtggtg gtggaggcgc tggaatgtta ggcacgggag aaggtggtgg
     3301 cggcggtgcc gccggtataa tttgttctgg tttagtttgt tcgcgcacga ttgtgggcac
     3361 cggcgcaggc gccgctggct gcacaacgga aggtcgtctg cttcgaggca gcgcttgggg
     3421 tggtggcaat tcaatattat aattggaata caaatcgtaa aaatctgcta taagcattgt
     3481 aatttcgcta tcgtttaccg tgccgatatt taacaaccgc tcaatgtaag caattgtatt
     3541 gtaaagagat tgtctcaagc tcgcacgccg ataacaagcc ttttcatttt tactacagca
     3601 ttgtagtggc gagacacttc gctgtcgtcg acgtacatgt atgctttgtt gtcaaaaacg
     3661 tcgttggcaa gctttaaaat atttaaaaga acatctctgt tcagcaccac tgtgttgtcg
     3721 taaatgttgt ttttgataat ttgcgcttcc gcagtatcga cacgttcaaa aaattgatgc
     3781 gcatcaattt tgttgttcct attattgaat aaataagatt gtacagattc atatctacga
     3841 ttcgtcatgg ccaccacaaa tgctacgctg caaacgctgg tacaatttta cgaaaactgc
     3901 aaaaacgtca aaactcggta taaaataatc aacgggcgct ttggcaaaat atctatttta
     3961 tcgcacaagc ccactagcaa attgtatttg cagaaaacaa tttcggcgca caattttaac
     4021 gctgacgaaa taaaagttca ccagttaatg agcgaccacc caaattttat aaaaatctat
     4081 tttaatcacg gttccatcaa caaccaagtg atcgtgatgg actacattga ctgtcccgat
     4141 ttatttgaaa cactacaaat taaaggcgag ctttcgtacc aacttgttag caatattatt
     4201 agacagctgt gtgaagcgct caacgatttg cacaagcaca atttcataca caacgacata
     4261 aaactcgaaa atgtcttata tttcgaagca cttgatcgcg tgtatgtttg cgattacgga
     4321 ttgtgcaaac acgaaaactc acttagcgtg cacgacggca cgttggagta ttttagtccg
     4381 gaaaaaattc gacaccacaa ctatgcacgt tcgtttgact ggtacgccgt cggcgtgtta
     4441 acatacaagt tgctaaccgg cggccaattc gtaatcatgg tcatagctgt ttcctgtgtg
     4501 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     4561 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     4621 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     4681 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     4741 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     4801 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     4861 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     4921 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4981 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     5041 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     5101 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     5161 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     5221 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     5281 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     5341 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     5401 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     5461 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     5521 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     5581 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     5641 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     5701 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     5761 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     5821 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     5881 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     5941 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     6001 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     6061 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     6121 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     6181 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     6241 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     6301 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     6361 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     6421 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     6481 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     6541 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     6601 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     6661 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     6721 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     6781 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     6841 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     6901 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
     6961 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     7021 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     7081 taaaacgacg gccagtgcca agcttggctc gtaaagcgag ttgaaggatc atatttagtt
     7141 gcgtttatga gataagattg aaagcacgtg taaaatgttt cccgcgcgtt ggcacaacta
     7201 tttacaatgc ggccaagtta taaaagattc taatctgata tgttttaaaa cacctttgcg
     7261 gcccgagttg tttgcgtacg tgactagcga agaagatatg tggaccgcag aacagatagt
     7321 aaaacaaaac cctagtattg gagcaataat cgatttaacc aacacgtcta aatattatga
     7381 tggtgtgcat tttttgcggg cgggcctgtt atacaaaaaa attcaagtac ctggccagac
     7441 tttgccgcct gaaagcatag ttcaagaatt tattgacacg gtaaaagaat ttacagaaaa
     7501 gtgtcccggc atgttggtgg gcgtgcactg cacacacggt attaatcgca ccggttacat
     7561 ggtgtgcaga tatttaatgc acaccctggg tattgcgccg caggaagcca tagatagatt
     7621 cgaaaaagcc agaggtcaca aaattgaaag acaaaattac gttcaagatt tattaattta
     7681 attaatatta tttgcattct ttaacaaata ctttatccta ttttcaaatt gttgcgcttc
     7741 ttccagcgaa ccaaaactat gcttcgcttg ctccgtttag cttgtagccg atcagtggcg
     7801 ttgttccaat cgacggtagg attaggccgg atattctcca ccacaatgtt ggcaacgttg
     7861 atgttacgtt tatgcttttg gttttccacg tacgtctttt ggccggtaat agccgtaaac
     7921 gtagtgccgt cgcgcgtcac gcacaacacc ggatgtttgc gcttgtccgc ggggtattga
     7981 accgcgcgat ccgacaaatc caccactttg gcaactaaat cggtgacctg cgcgtctttt
     8041 ttctgcatta tttcgtcttt cttttgcatg gtttcctgga agccggtgta catgcggttt
     8101 agatcagtca tgacgcgcgt gacctgcaaa tctttggcct cgatctgctt gtccttgatg
     8161 gcaacgatgc gttcaataaa ctcttgtttt ttaacaagtt cctcggtttt ttgcgccacc
     8221 accgcttgca gcgcgtttgt gtgctcggtg aatgtcgcaa tcagcttagt caccaactgt
     8281 ttgctctcct cctcccgttg tttgatcgcg ggatcgtact tgccggtgca gagcacttga
     8341 ggaattactt cttctaaaag ccattcttgt aattctatgg cgtaaggcaa tttggacttc
     8401 ataatcagct gaatcacgcc ggatttagta atgagcactg tatgcggctg caaatacagc
     8461 gggtcgcccc ttttcacgac gctgttagag gtagggcccc cattttggat ggtctgctca
     8521 aataacgatt tgtatttatt gtctacatga acacgtatag ctttatcaca aactgtatat
     8581 tttaaactgt tagcgacgtc cttggccacg aaccggacct gttggtcgcg ctctagcacg
     8641 taccgcaggt tgaacgtatc ttctccaaat ttaaattctc caattttaac gcgagccatt
     8701 ttgatacacg tgtgtcgatt ttgcaacaac tattgttttt taacgcaaac taaacttatt
     8761 gtggtaagca ataattaaat atgggggaac atgcgccgct acaacactcg tcgttatgaa
     8821 cgcagacggc gccggtctcg gcgcaagcgg ctaaaacgtg ttgcgcgttc aacgcggcaa
     8881 acatcgcaaa agccaatagt acagttttga tttgcatatt aacggcgatt ttttaaatta
     8941 tcttatttaa taaatagtta tgacgcctac aactccccgc ccgcgttgac tcgctgcacc
     9001 tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg
     9061 accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa
     9121 ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca gttgggttgt
     9181 ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg catgcaagcc
     9241 gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct tttaatcatg
     9301 ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat gttttctttg
     9361 tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta agttagtttc
     9421 atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat taacaatgcg
     9481 cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga attaaatagc
     9541 ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga ttgtaataag
     9601 tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa aagaactgcc
     9661 gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc caatcgaccg
     9721 ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa
     9781 cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat atttaattga
     9841 tcccgatgat tttattgata aattgaccct aactccatac acggtattct acaatggcgg
     9901 ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc cgcccactat
     9961 taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga
    10021 atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc
    10081 gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta
    10141 caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga
    10201 aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg
    10261 tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa
    10321 aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat
    10381 ttgtaattat tgaataataa aacaattata aatgtcaaat ttgtttttta ttaacgatac
    10441 aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa
    10501 tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata
    10561 attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt
    10621 tcatttttct cctcataaaa attaacatag ttattatcgt atccatatat gtatctatcg
    10681 tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta
    10741 ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag
    10801 tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac
    10861 aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
    10921 accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct
    10981 cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta
    11041 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgat
//
