LOCUS       sequence-221610-        8169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of exogenous LAP tagged MPS1 delta TPR (TPR is deleted).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8169)
  AUTHORS   Nijenhuis W, von Castelmur E, Littler D, De Marco V, Tromer E, 
            Vleugel M, van Osch MH, Snel B, Perrakis A, Kops GJ
  TITLE     A TPR domain-containing N-terminal module of MPS1 is required for 
            its kinetochore localization by Aurora B.
  JOURNAL   J Cell Biol. 2013 Apr 15;201(2):217-31. doi: 10.1083/jcb.201210033. 
            Epub 2013 Apr 8.
  PUBMED    23569217
REFERENCE   2  (bases 1 to 8169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201210033"; journalName: "J Cell Biol"; date: 
            "2013-04-15- 15"; volume: "201"; issue: "2"; pages: "217-31"
COMMENT     Sequence Label: pCDNA5-LAP-MPS1-dTPR
FEATURES             Location/Qualifiers
     source          1..8169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        375..578
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     529..549
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    580..598
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    601..619
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     629..653
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      778..787
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(784..786,787..789,790..1500)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(829..850)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1090..1109)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1437..1458
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1546..1566
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1579..1623
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(3881..3898)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3887..4111
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4244..4263)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4395..4442
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4450..5472
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4450..5472
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5602..5723
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5639..5658)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5693..5712
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5772..5788)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5772..5788)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5785..5807)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5796..5812
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5820..5826,5827..5844,5845..5850))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5865..5886
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6003..6020)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6174..6762)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6254..6273)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6933..7724,7725..7793))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7556..7575
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7794..7898)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7973..7992)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8164..8169,1..374)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
       61 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      121 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      181 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      241 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      301 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      361 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      421 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      481 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      541 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      601 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      661 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      721 ctctagcgtt taaacttaag cttggtaccg agctcggatc cgctagcgct accggtcgcc
      781 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
      841 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
      901 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
      961 accctcgtga ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg
     1021 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     1081 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     1141 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     1201 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     1261 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     1321 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     1381 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     1441 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     1501 tccggagggg atcggtggag ctccaccggt ggcggccgct ctagagagaa tctttatttt
     1561 cagggcgccg ccaaattcaa agaaaccgct gctgctaaat tcgaacgcca gcacatggac
     1621 agcggaggtg gaggtactag tctcgagatg gaatccgagg atttaagtgg cagagaattg
     1681 acaattgatt ccataatgaa caaagtgaga gacattaaaa ataagtttaa aaatgaagac
     1741 cttactgatg aactaagctt gaataaaatt tctgctgata ctacagataa ctcgggaact
     1801 gttaaccaaa ttatgatgat ggcaaacaac ccaatcgccc agctgctttc agaggaggaa
     1861 aagaagaatt tatcagcatc tacggtatta actgcccaag aatcattttc cggttcactt
     1921 gggcatttac agaataggaa caacagttgt gattccagag gacagactac taaagccagg
     1981 tttttatatg gagagaacat gccaccacaa gatgcagaaa taggttaccg gaattcattg
     2041 agacaaacta acaaaactaa acagtcatgc ccatttggaa gagtcccagt taaccttcta
     2101 aatagcccag attgtgatgt gaagacggac gattcagttg taccttgttt tatgaaaaga
     2161 caaacctcta gatcagaatg ccgagatttg gttgtgcctg gatctaaacc aagtggaaat
     2221 gattcctgtg aattaagaaa tttaaagtct gttcaaaata gtcatttcaa ggaacctctg
     2281 gtgtcagatg aaaagagttc tgaacttatt attactgatt caataaccct gaagaataaa
     2341 acggaatcaa gtcttctagc taaattagaa gaaactaaag agtatcaaga accagaggtt
     2401 ccagagagta accagaaaca gtggcaatct aagagaaagt cagagtgtat taaccagaat
     2461 cctgctgcat cttcaaatca ctggcagatt ccggagttag cccgaaaagt taatacagag
     2521 cagaaacata ccacttttga gcaacctgtc ttttcagttt caaaacagtc accaccaata
     2581 tcaacatcta aatggtttga cccaaaatct atttgtaaga caccaagcag caataccttg
     2641 gatgattaca tgagctgttt tagaactcca gttgtaaaga atgactttcc acctgcttgt
     2701 cagttgtcaa caccttatgg ccaacctgcc tgtttccagc agcaacagca tcaaatactt
     2761 gccactccac ttcaaaattt acaggtttta gcatcttctt cagcaaatga atgcatttcg
     2821 gttaaaggaa gaatttattc catattaaag cagataggaa gtggaggttc aagcaaggta
     2881 tttcaggtgt taaatgaaaa gaaacagata tatgctataa aatatgtgaa cttagaagaa
     2941 gcagataacc aaactcttga tagttaccgg aacgaaatag cttatttgaa taaactacaa
     3001 caacacagtg ataagatcat ccgactttat gattatgaaa tcacggacca gtacatctac
     3061 atggtaatgg agtgtggaaa tattgatctt aatagttggc ttaaaaagaa aaaatccatt
     3121 gatccatggg aacgcaagag ttactggaaa aatatgttag aggcagttca cacaatccat
     3181 caacatggca ttgttcacag tgatcttaaa ccagctaact ttctgatagt tgatggaatg
     3241 ctaaagctaa ttgattttgg gattgcaaac caaatgcaac cagatacaac aagtgttgtt
     3301 aaagattctc aggttggcac agttaattat atgccaccag aagcaatcaa agatatgtct
     3361 tcctccagag agaatgggaa atctaagtca aagataagcc ccaaaagtga tgtttggtcc
     3421 ttaggatgta ttttgtacta tatgacttac gggaaaacac catttcagca gataattaat
     3481 cagatttcta aattacatgc cataattgat cctaatcatg aaattgaatt tcccgatatt
     3541 ccagagaaag atcttcaaga tgtgttaaag tgttgtttaa aaagggaccc aaaacagagg
     3601 atatccattc ctgagctcct ggctcatcca tatgttcaaa ttcaaactca tccagttaac
     3661 caaatggcca agggaaccac tgaagaaatg aaatatgttc tgggccaact tgttggtctg
     3721 aattctccta actccatttt gaaagctgct aaaactttat atgaacacta tagtggtggt
     3781 gaaagtcata attcttcatc ctccaagact tttgaaaaaa aaaggggaaa aaaatgagtc
     3841 gagcatgcat ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     3901 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     3961 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     4021 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     4081 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     4141 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4201 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4261 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4321 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     4381 ggttcacgta cctagaagtt cctattccga agttcctatt ctctagaaag tataggaact
     4441 tccttggcca aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag
     4501 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
     4561 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
     4621 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
     4681 gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
     4741 acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc
     4801 atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg
     4861 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
     4921 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
     4981 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
     5041 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
     5101 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
     5161 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
     5221 tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
     5281 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
     5341 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
     5401 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg
     5461 gcaaaggaat agcacgtact acgagatttc gattccaccg ccgccttcta tgaaaggttg
     5521 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     5581 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     5641 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     5701 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     5761 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     5821 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     5881 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     5941 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     6001 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     6061 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6121 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6181 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6241 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6301 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6361 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6421 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6481 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6541 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6601 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6661 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6721 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     6781 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     6841 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     6901 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     6961 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     7021 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     7081 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     7141 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     7201 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     7261 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     7321 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     7381 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     7441 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     7501 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     7561 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     7621 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     7681 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     7741 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     7801 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     7861 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     7921 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     7981 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     8041 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     8101 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     8161 gttgacatt
//
