LOCUS       sequence-221614-        9475 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This lentiviral plasmid expresses MCV tumor T antigens (truncated LT
            and small T) using the viral NCCR (non-coding control region). The 
            sequence was amplifeied from MCV.R17a and truncated LT=428 aa..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9475)
  AUTHORS   Arora R, Rekhi B, Chandrani P, Krishna S, Dutt A
  TITLE     Merkel cell polyomavirus is implicated in a subset of Merkel cell 
            carcinomas, in the Indian subcontinent.
  JOURNAL   Microb Pathog. 2019 Dec;137:103778. doi: 
            10.1016/j.micpath.2019.103778. Epub 2019 Oct 7.
  PUBMED    31600537
REFERENCE   2  (bases 1 to 9475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Pathog."; date: "2019-12"; volume: "137:103778. doi"; pages: " 
            10.1016/j.micpath.2019.103778. Epub 2019 Oct 7"
COMMENT     Sequence Label: pLenti.puro.MCV.ER.RAZ.2
FEATURES             Location/Qualifiers
     source          1..9475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    3908..4496
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3961..3981)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4379..4390)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4510..5009
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4545..4568)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4942..4961
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5030..5629
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5030..5049)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5526..5546
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5767..6000
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6072..6193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6109..6128)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6163..6182
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6233..6368
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6388..6407)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6389..6407)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6417..6434)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6417..6433)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6426..6448)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6575..7030
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6662..6681)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6872..6893
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7056..7160
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7161..7229,7230..8021)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7379..7398)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8192..8780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8681..8700
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8934..8951
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9068..9089
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9104..9109,9110..9127,9128..9134)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9142..9158
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9147..9169
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9166..9182
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9166..9182
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9201..9221
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9203..9221
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9249..9475
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 ttcaaaattt tatcgatcct gaaaaataaa taaggatact tactctttta atgtcctcct
     1741 ccctttgtaa gagaaaaaaa agcctccggg cctcccttgt tgaaaaaaag ttaagagtct
     1801 tccgtctccc tcccaaacag aaagaaaaaa agttttgttt atcagtcaaa ctccgcctct
     1861 ccaggaaatg agtcaatgcc agaaaccctg cagcaataaa agttcaatca tgtaaccaca
     1921 acttggctgc ctaggtgact tttttttttc aagttggcag aggcttgggg ctcctagcct
     1981 ccgaggcctc tggaaaaaaa gagagaggcc tctgaggctt aagaggctta attagcaaaa
     2041 aaggcagtat ctaagggcag atcccaaggg cgggaaactg cagtataaaa accactcctt
     2101 agtgaggtag ctcatttgct cctctgctct ttctgcaaac tccttctgca tatagacaag
     2161 atggatttag tcctaaatag gaaagaaaga gaggctctct gcaagctttt agagattgct
     2221 cctaattgtt atggcaacat ccctctgatg aaagctgctt tcaaaagaag ctgcttaaag
     2281 catcaccctg ataaaggggg aaatcctgtt ataatgatgg aattgaacac cctttggagc
     2341 aaattccagc aaaatatcca caagctcaga agtgacttct ctatgtttga tgaggtcagt
     2401 acaaaatttc cttgggaaga atatggaact ttaaaggatt atatgcaaag tggatataat
     2461 gctagatttt gcagaggtcc tgggtgcatg cttaagcaac ttagagattc taagtgcgct
     2521 tgtattagct gtaagttgtc tcgccagcat tgtagtctaa aaactttaaa gcaaaaaaac
     2581 tgtctgacgt ggggagagtg tttttgctat cagtgcttta ttctttggtt tggatttcct
     2641 cctacttggg aaagttttga ctggtggcaa aaaactttag aagaaactga ctactgctta
     2701 ctgcatctgc accttttcta gactcctact tccttcctct gtaagtatta gatatggaaa
     2761 agtctataag gcaaaatatc aaagaaaggt tatttatgac agattttctg tactttccca
     2821 tctaggttga cgaggcccct atatatggga ccactaaatt caaagaatgg tggagatcag
     2881 gaggattcag cttcgggaag gcatacgaat atgggcccaa tccacacggg accaactcaa
     2941 gatccagaaa gccttcctcc aatgcatcca ggggagcccc cagtggaagc tcaccacccc
     3001 acagccagag ctcttcctct gggtatgggt ccttctcagc gtcccaggct tcagactccc
     3061 agtccagagg acccgatata cctcccgaac accatgagga acccacctca tcctctggat
     3121 ccagtagcag agaggagacc accaattcag gaagagaatc cagcacaccc aatggaacca
     3181 gtgtacctag aaattcttcc agaacggatg gcacctggga ggatctcttc tgcgatgaat
     3241 cactttcctc ccctgagcct ccctcgtcct ctgaggagcc tgaggagccc ccctcctcaa
     3301 gaagctcgcc ccggcagccc ccgtcttcct ctgccgagga ggcctcgtca tctcagttta
     3361 cagatgagga atacagatcc tcctccttca ccaccccgaa gacccctcct ccattctcaa
     3421 gaaagcgaaa atttgggggg tcccgaagct ctgcaagctc tgctagttca gcaagtttta
     3481 caagcactcc accaaagcca aaaaagaaca gagaaactcc tgttcctact gattttccta
     3541 ttgatctttc tgattatctt agccatgctg tatatagtaa taaaacagta agttgttttg
     3601 ccatttatac tacttctgat aaagctatag agttatatga taagattgag aaatttaaag
     3661 ttgattttaa aagcaggcat gcctgtgaat taggatgtat tttattgttt ataactttat
     3721 caaagcatag agtatctgct attaagaatt tttgctctac cttctgcact ataagctttt
     3781 taatttgtaa aggagtgaat aagatgcctg aaatgtataa taatttatgc aagccccctt
     3841 acaaattact gcaagagaat aagccactgc tcaattagga tatccagcac agtggcggcc
     3901 gctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     3961 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     4021 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     4081 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     4141 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     4201 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     4261 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     4321 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     4381 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     4441 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     4501 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
     4561 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
     4621 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
     4681 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
     4741 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
     4801 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
     4861 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
     4921 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
     4981 tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta
     5041 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     5101 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     5161 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     5221 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     5281 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     5341 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     5401 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     5461 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     5521 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     5581 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca
     5641 cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatctcgag ggcccggtac
     5701 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     5761 ggggactgga agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg
     5821 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     5881 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     5941 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     6001 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     6061 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     6121 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     6181 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     6241 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6301 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     6361 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     6421 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     6481 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     6541 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     6601 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6661 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6721 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6781 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6841 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6901 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     6961 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     7021 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7081 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7141 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     7201 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     7261 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     7321 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     7381 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     7441 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     7501 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     7561 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7621 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7681 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     7741 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7801 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7861 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7921 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7981 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     8041 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     8101 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8161 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     8221 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     8281 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     8341 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     8401 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     8461 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8521 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8581 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8641 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8701 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8761 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8821 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     8881 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     8941 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     9001 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     9061 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     9121 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     9181 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     9241 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
     9301 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     9361 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
     9421 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaac
//
