LOCUS       sequence-221645-        1353 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For Calcium imaging, expression of G-CaMP6 under the expression of a
            npr-4 promoter (AVA and RIV interneurons).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Hori S, Oda S, Suehiro Y, Iino Y, Mitani S
  TITLE     OFF-responses of interneurons optimize avoidance behaviors depending
            on stimulus strength via electrical synapses.
  JOURNAL   PLoS Genet. 2018 Jun 25;14(6):e1007477. doi: 
            10.1371/journal.pgen.1007477. eCollection 2018 Jun.
  PUBMED    29939997
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2018-06-25"; volume: "14(6):e1007477. doi"; pages: "
            10.1371/journal.pgen.1007477. eCollection 2018 Jun"
COMMENT     Sequence Label: pPD_Pnpr-4::G-CaMP6s
FEATURES             Location/Qualifiers
     source          1..1353
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..9,10..27,28..30,31..63,64..66,67..90,91..117,
                     118..174,175..180,181..450,451..468,469..903,904..909,
                     910..1353)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      97..106
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     387..408
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(514..535)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(775..794)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atgggttctc atcatcatca tcatcatggt atggctagca tgactggtgg acagcaaatg
       61 ggtcgggatc tgtacgacga tgacgataag gatctcgcca ccatggtcga ctcatcacgt
      121 cgtaagtgga ataagacagg tcacgcagtc agagctatag gtcggctgag ctcactcgag
      181 aacgtctata tcaaggccga caagcagaag aacggcatca aggcgaactt ccacatccgc
      241 cacaacatcg aggacggcgg cgtgcagctc gcctaccact accagcagaa cacccccatc
      301 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcgtgcagtc caaactttcg
      361 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      421 atcactctcg gcatggacga gctgtacaag ggcggtaccg gagggagcat ggtgagcaag
      481 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      541 ggccacaagt tcagcgtgtc cggcgagggt gagggcgatg ccacctacgg caagctgacc
      601 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      661 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
      721 ttcaagtccg ccatgcccga aggctacatc caggagcgca ccatcttctt caaggacgac
      781 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      841 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      901 aacctgccgg accaactgac tgaagagcag atcgcagaat ttaaagaggc tttctcccta
      961 tttgacaagg acggggatgg gacaataaca accaaggagc tggggacggt gatgcggtct
     1021 ctggggcaga accccacaga agcagagctg caggacatga tcaatgaagt agatgccgac
     1081 ggtgacggca caatcgactt ccctgagttc ctgacaatga tggcaagaaa aatgaaatac
     1141 agggacacgg aagaagaaat tagagaagcg ttcggtgtgt ttgataagga tggcaatggc
     1201 tacatcagtg cagcagagct tcgccacgtg atgacaaacc ttggagagaa gttaacagat
     1261 gaagaggttg atgaaatgat cagggaagca gacatcgatg gggatggtca ggtaaactac
     1321 gaagagtttg tacaaatgat gacagcgaag tga
//
