LOCUS       sequence-221650-        7786 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  protein production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7786)
  AUTHORS   Jelluma N, Brenkman AB, van den Broek NJ, Cruijsen CW, van Osch MH, 
            Lens SM, Medema RH, Kops GJ
  TITLE     Mps1 phosphorylates Borealin to control Aurora B activity and 
            chromosome alignment.
  JOURNAL   Cell. 2008 Jan 25;132(2):233-46. doi: 10.1016/j.cell.2007.11.046.
  PUBMED    18243099
REFERENCE   2  (bases 1 to 7786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2007.11"; journalName: "Cell"; date: "2008-01-25- 
            25"; volume: "132"; issue: "2"; pages: "233-46"
COMMENT     Sequence Label: pFastBac-His6-Mps1-WT
FEATURES             Location/Qualifiers
     source          1..7786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        43..134
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     59..76
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             201..218
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             240..260
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    3434..3568
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3471..3490)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3525..3544
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3597..3762
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3946..4400
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4033..4052)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4242..4263
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4426..4530
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4531..4599,4600..5391)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4749..4768)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5562..6150
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6051..6070
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6304..6321
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  6455..6679
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(6746..7279)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(7449..7454,7455..7471,7472..7477))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 tagttctagt ggttggctac gtatactccg gaatattaat agatcatgga gataattaaa
       61 atgataacca tctcgcaaat aaataagtat tttactgttt tcgtaacagt tttgtaataa
      121 aaaaacctat aaatattccg gattattcat accgtcccac catcgggcgc ggatctcggt
      181 ccgaaaccat gtcgtactac catcaccatc accatcacga ttacgatatc ccaacgaccg
      241 aaaacctgta ttttcagggc gccatggatc cggaattcaa aggcctacgt cgacgagctc
      301 aactagtgcg gccgcgaatc ggcacgaggg tagaaatgga atccgaggat ttaagtggca
      361 gagaattgac aattgattcc ataatgaaca aagtgagaga cattaaaaat aagtttaaaa
      421 atgaagacct tactgatgaa ctaagcttga ataaaatttc tgctgatact acagataact
      481 cgggaactgt taaccaaatt atgatgatgg caaacaaccc agaggactgg ttgagtttgt
      541 tgctcaaact agagaaaaac agtgttccgc taagtgatgc tcttttaaat aaattgattg
      601 gtcgttacag tcaagcaatt gaagcgcttc ccccagataa atatggccaa aatgagagtt
      661 ttgctagaat tcaagtgaga tttgctgaat taaaagctat tcaagagcca gatgatgcac
      721 gtgactactt tcaaatggcc agagcaaact gcaagaaatt tgcttttgtt catatatctt
      781 ttgcacaatt tgaactgtca caaggtaatg tcaaaaaaag taaacaactt cttcaaaaag
      841 ctgtagaacg tggagcagta ccactagaaa tgctggaaat tgccctgcgg aatttaaacc
      901 tccaaaaaaa gcagctgctt tcagaggagg aaaagaagaa tttatcagca tctacggtat
      961 taactgccca agaatcattt tccggttcac ttgggcattt acagaatagg aacaacagtt
     1021 gtgattccag aggacagact actaaagcca ggtttttata tggagagaac atgccaccac
     1081 aagatgcaga aataggttac cggaattcat tgagacaaac taacaaaact aaacagtcat
     1141 gcccatttgg aagagtccca gttaaccttc taaatagccc agattgtgat gtgaagacag
     1201 atgattcagt tgtaccttgt tttatgaaaa gacaaacctc tagatcagaa tgccgagatt
     1261 tggttgtgcc tggatctaaa ccaagtggaa atgattcctg tgaattaaga aatttaaagt
     1321 ctgttcaaaa tagtcatttc aaggaacctc tggtgtcaga tgaaaagagt tctgaactta
     1381 ttattactga ttcaataacc ctgaagaata aaacggaatc aagtcttcta gctaaattag
     1441 aagaaactaa agagtatcaa gaaccagagg ttccagagag taaccagaaa cagtggcaat
     1501 ctaagagaaa gtcagagtgt attaaccaga atcctgctgc atcttcaaat cactggcaga
     1561 ttccggagtt agcccgaaaa gttaatacag agcagaaaca taccactttt gagcaacctg
     1621 tcttttcagt ttcaaaacag tcaccaccaa tatcaacatc taaatggttt gacccaaaat
     1681 ctatttgtaa gacaccaagc agcaatacct tggatgatta catgagctgt tttagaactc
     1741 cagttgtaaa gaatgacttt ccacctgctt gtcagttgtc aacaccttat ggccaacctg
     1801 cctgtttcca gcagcaacag catcaaatac ttgccactcc acttcaaaat ttacaggttt
     1861 tagcatcttc ttcagcaaat gaatgcattt cggttaaagg aagaatttat tccatattaa
     1921 agcagatagg aagtggaggt tcaagcaagg tatttcaggt gttaaatgaa aagaaacaga
     1981 tatatgctat aaaatatgtg aacttagaag aagcagataa ccaaactctt gatagttacc
     2041 ggaacgaaat agcttatttg aataaactac aacaacacag tgataagatc atccgacttt
     2101 atgattatga aatcacggac cagtacatct acatggtaat ggagtgtgga aatattgatc
     2161 ttaatagttg gcttaaaaag aaaaaatcca ttgatccatg ggaacgcaag agttactgga
     2221 aaaatatgtt agaggcagtt cacacaatcc atcaacatgg cattgttcac agtgatctta
     2281 aaccagctaa ctttctgata gttgatggaa tgctaaagct aattgatttt gggattgcaa
     2341 accaaatgca accagataca acaagtgttg ttaaagattc tcaggttggc acagttaatt
     2401 atatgccacc agaagcaatc aaagatatgt cttcctccag agagaatggg aaatctaagt
     2461 caaagataag ccccaaaagt gatgtttggt ccttaggatg tattttgtac tatatgactt
     2521 acgggaaaac accatttcag cagataatta atcagatttc taaattacat gccataattg
     2581 atcctaatca tgaaattgaa tttcccgata ttccagagaa agatcttcaa gatgtgttaa
     2641 agtgttgttt aaaaagggac ccaaaacaga ggatatccat tcctgagctc ctggctcatc
     2701 catatgttca aattcaaact catccagtta accaaatggc caagggaacc actgaagaaa
     2761 tgaaatatgt tctgggccaa cttgttggtc tgaattctcc taactccatt ttgaaagctg
     2821 ctaaaacttt atatgaacac tatagtggtg gtgaaagtca taattcttca tcctccaaga
     2881 cttttgaaaa aaaaagggga aaaaaatgat ttgcagttat tcgtaatgtc agataccacc
     2941 tataaaatat attggactgt tatactcttg aatccctgtg gaaatctaca tttgaagaca
     3001 acatcactct gaagtgttat cagcaaaaaa aattcagtag attatcttta aaagaaaact
     3061 gtaaaaatag caaccactta tggcactgta tatattgtag acttgttttc tctgttttat
     3121 gctcttgtgt aatctacttg acatcatttt actcttggaa tagtgggtgg atagcaagta
     3181 tattctaaaa aactttgtaa ataaagtttt gtggctaaaa tgacaaaaaa aaaaaaaaaa
     3241 aactcgactc tagattgcgg ccgctttcga atctagagcc tgcagtctcg aggcatgcgg
     3301 taccaagctt gtcgagaagt actagaggat cataatcagc cataccacat ttgtagaggt
     3361 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     3421 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     3481 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     3541 catcaatgta tcttatcatg tctggatctg atcactgctt gagcctagga gatccgaacc
     3601 agataagtga aatctagttc caaactattt tgtcattttt aattttcgta ttagcttacg
     3661 acgctacacc cagttcccat ctattttgtc actcttccct aaataatcct taaaaactcc
     3721 atttccaccc ctcccagttc ccaactattt tgtccgccca cagcggggca tttttcttcc
     3781 tgttatgttt ttaatcaaac atcctgccaa ctccatgtga caaaccgtca tcttcggcta
     3841 ctttttctct gtcacagaat gaaaattttt ctgtcatctc ttcgttatta atgtttgtaa
     3901 ttgactgaat atcaacgctt atttgcagcc tgaatggcga atgggacgcg ccctgtagcg
     3961 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     4021 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     4081 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     4141 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     4201 cggtttttcg ccctttgacg ttggagtcca cgttcttaat agtggactct tgttccaaac
     4261 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     4321 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     4381 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4441 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4501 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     4561 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     4621 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     4681 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     4741 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     4801 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4861 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4921 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     4981 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     5041 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     5101 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     5161 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     5221 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     5281 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5341 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5401 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5461 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     5521 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5581 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5641 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5701 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5761 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5821 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5881 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5941 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6001 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6061 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6121 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6181 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     6241 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     6301 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
     6361 catctgtgcg gtatttcaca ccgcatagac cagccgcgta acctggcaaa atcggttacg
     6421 gttgagtaat aaatggatgc cctgcgtaag cgggtgtggg cggacaataa agtcttaaac
     6481 tgaacaaaat agatctaaac tatgacaata aagtcttaaa ctagacagaa tagttgtaaa
     6541 ctgaaatcag tccagttatg ctgtgaaaaa gcatactgga cttttgttat ggctaaagca
     6601 aactcttcat tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg tggccaaggg
     6661 catggtaaag actatattcg cggcgttgtg acaatttacc gaacaactcc gcggccggga
     6721 agccgatctc ggcttgaacg aattgttagg tggcggtact tgggtcgata tcaaagtgca
     6781 tcacttcttc ccgtatgccc aactttgtat agagagccac tgcgggatcg tcaccgtaat
     6841 ctgcttgcac gtagatcaca taagcaccaa gcgcgttggc ctcatgcttg aggagattga
     6901 tgagcgcggt ggcaatgccc tgcctccggt gctcgccgga gactgcgaga tcatagatat
     6961 agatctcact acgcggctgc tcaaacttgg gcagaacgta agccgcgaga gcgccaacaa
     7021 ccgcttcttg gtcgaaggca gcaagcgcga tgaatgtctt actacggagc aagttcccga
     7081 ggtaatcgga gtccggctga tgttgggagt aggtggctac gtctccgaac tcacgaccga
     7141 aaagatcaag agcagcccgc atggatttga cttggtcagg gccgagccta catgtgcgaa
     7201 tgatgcccat acttgagcca cctaactttg ttttagggcg actgccctgc tgcgtaacat
     7261 cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg
     7321 cccgaggcat agactgtaca aaaaaacagt cataacaagc catgaaaacc gccactgcgc
     7381 cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact
     7441 tgcattacag tttacgaacc gaacaggctt atgtcaactg ggttcgtgcc ttcatccgtt
     7501 tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga agtcgaggca tttctgtcct
     7561 ggctggcgaa cgagcgcaag gtttcggtct ccacgcatcg tcaggcattg gcggccttgc
     7621 tgttcttcta cggcaaggtg ctgtgcacgg atctgccctg gcttcaggag atcggaagac
     7681 ctcggccgtc gcggcgcttg ccggtggtgc tgaccccgga tgaagtggtt cgcatcctcg
     7741 gttttctgga aggcgagcat cgtttgttcg cccaggactc tagcta
//
