LOCUS       sequence-221651-        7786 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  protein production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7786)
  AUTHORS   Jelluma N, Brenkman AB, van den Broek NJ, Cruijsen CW, van Osch MH, 
            Lens SM, Medema RH, Kops GJ
  TITLE     Mps1 phosphorylates Borealin to control Aurora B activity and 
            chromosome alignment.
  JOURNAL   Cell. 2008 Jan 25;132(2):233-46. doi: 10.1016/j.cell.2007.11.046.
  PUBMED    18243099
REFERENCE   2  (bases 1 to 7786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2007.11"; journalName: "Cell"; date: "2008-01-25- 
            25"; volume: "132"; issue: "2"; pages: "233-46"
COMMENT     Sequence Label: pFastBac-His6-Mps1-KD
FEATURES             Location/Qualifiers
     source          1..7786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        140..231
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     156..173
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             298..315
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             337..357
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    3531..3665
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3568..3587)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3622..3641
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3694..3859
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4043..4497
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4130..4149)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4339..4360
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4523..4627
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4628..4696,4697..5488)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4846..4865)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5659..6247
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6148..6167
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6401..6418
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  6552..6776
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(6843..7376)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(7546..7551,7552..7568,7569..7574))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 cgcggcgctt gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg
       61 aaggcgagca tcgtttgttc gcccaggact ctagctatag ttctagtggt tggctacgta
      121 tactccggaa tattaataga tcatggagat aattaaaatg ataaccatct cgcaaataaa
      181 taagtatttt actgttttcg taacagtttt gtaataaaaa aacctataaa tattccggat
      241 tattcatacc gtcccaccat cgggcgcgga tctcggtccg aaaccatgtc gtactaccat
      301 caccatcacc atcacgatta cgatatccca acgaccgaaa acctgtattt tcagggcgcc
      361 atggatccgg aattcaaagg cctacgtcga cgagctcaac tagtgcggcc gcgaatcggc
      421 acgagggtag aaatggaatc cgaggattta agtggcagag aattgacaat tgattccata
      481 atgaacaaag tgagagacat taaaaataag tttaaaaatg aagaccttac tgatgaacta
      541 agcttgaata aaatttctgc tgatactaca gataactcgg gaactgttaa ccaaattatg
      601 atgatggcaa acaacccaga ggactggttg agtttgttgc tcaaactaga gaaaaacagt
      661 gttccgctaa gtgatgctct tttaaataaa ttgattggtc gttacagtca agcaattgaa
      721 gcgcttcccc cagataaata tggccaaaat gagagttttg ctagaattca agtgagattt
      781 gctgaattaa aagctattca agagccagat gatgcacgtg actactttca aatggccaga
      841 gcaaactgca agaaatttgc ttttgttcat atatcttttg cacaatttga actgtcacaa
      901 ggtaatgtca aaaaaagtaa acaacttctt caaaaagctg tagaacgtgg agcagtacca
      961 ctagaaatgc tggaaattgc cctgcggaat ttaaacctcc aaaaaaagca gctgctttca
     1021 gaggaggaaa agaagaattt atcagcatct acggtattaa ctgcccaaga atcattttcc
     1081 ggttcacttg ggcatttaca gaataggaac aacagttgtg attccagagg acagactact
     1141 aaagccaggt ttttatatgg agagaacatg ccaccacaag atgcagaaat aggttaccgg
     1201 aattcattga gacaaactaa caaaactaaa cagtcatgcc catttggaag agtcccagtt
     1261 aaccttctaa atagcccaga ttgtgatgtg aagacagatg attcagttgt accttgtttt
     1321 atgaaaagac aaacctctag atcagaatgc cgagatttgg ttgtgcctgg atctaaacca
     1381 agtggaaatg attcctgtga attaagaaat ttaaagtctg ttcaaaatag tcatttcaag
     1441 gaacctctgg tgtcagatga aaagagttct gaacttatta ttactgattc aataaccctg
     1501 aagaataaaa cggaatcaag tcttctagct aaattagaag aaactaaaga gtatcaagaa
     1561 ccagaggttc cagagagtaa ccagaaacag tggcaatcta agagaaagtc agagtgtatt
     1621 aaccagaatc ctgctgcatc ttcaaatcac tggcagattc cggagttagc ccgaaaagtt
     1681 aatacagagc agaaacatac cacttttgag caacctgtct tttcagtttc aaaacagtca
     1741 ccaccaatat caacatctaa atggtttgac ccaaaatcta tttgtaagac accaagcagc
     1801 aataccttgg atgattacat gagctgtttt agaactccag ttgtaaagaa tgactttcca
     1861 cctgcttgtc agttgtcaac accttatggc caacctgcct gtttccagca gcaacagcat
     1921 caaatacttg ccactccact tcaaaattta caggttttag catcttcttc agcaaatgaa
     1981 tgcatttcgg ttaaaggaag aatttattcc atattaaagc agataggaag tggaggttca
     2041 agcaaggtat ttcaggtgtt aaatgaaaag aaacagatat atgctataaa atatgtgaac
     2101 ttagaagaag cagataacca aactcttgat agttaccgga acgaaatagc ttatttgaat
     2161 aaactacaac aacacagtga taagatcatc cgactttatg attatgaaat cacggaccag
     2221 tacatctaca tggtaatgga gtgtggaaat attgatctta atagttggct taaaaagaaa
     2281 aaatccattg atccatggga acgcaagagt tactggaaaa atatgttaga ggcagttcac
     2341 acaatccatc aacatggcat tgttcacagt gatcttaaac cagctaactt tctgatagtt
     2401 gatggaatgc taaagctaat tgcttttggg attgcaaacc aaatgcaacc agatacaaca
     2461 agtgttgtta aagattctca ggttggcaca gttaattata tgccaccaga agcaatcaaa
     2521 gatatgtctt cctccagaga gaatgggaaa tctaagtcaa agataagccc caaaagtgat
     2581 gtttggtcct taggatgtat tttgtactat atgacttacg ggaaaacacc atttcagcag
     2641 ataattaatc agatttctaa attacatgcc ataattgatc ctaatcatga aattgaattt
     2701 cccgatattc cagagaaaga tcttcaagat gtgttaaagt gttgtttaaa aagggaccca
     2761 aaacagagga tatccattcc tgagctcctg gctcatccat atgttcaaat tcaaactcat
     2821 ccagttaacc aaatggccaa gggaaccact gaagaaatga aatatgttct gggccaactt
     2881 gttggtctga attctcctaa ctccattttg aaagctgcta aaactttata tgaacactat
     2941 agtggtggtg aaagtcataa ttcttcatcc tccaagactt ttgaaaaaaa aaggggaaaa
     3001 aaatgatttg cagttattcg taatgtcaga taccacctat aaaatatatt ggactgttat
     3061 actcttgaat ccctgtggaa atctacattt gaagacaaca tcactctgaa gtgttatcag
     3121 caaaaaaaat tcagtagatt atctttaaaa gaaaactgta aaaatagcaa ccacttatgg
     3181 cactgtatat attgtagact tgttttctct gttttatgct cttgtgtaat ctacttgaca
     3241 tcattttact cttggaatag tgggtggata gcaagtatat tctaaaaaac tttgtaaata
     3301 aagttttgtg gctaaaatga caaaaaaaaa aaaaaaaaac tcgactctag attgcggccg
     3361 ctttcgaatc tagagcctgc agtctcgagg catgcggtac caagcttgtc gagaagtact
     3421 agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     3481 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     3541 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     3601 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     3661 ggatctgatc actgcttgag cctaggagat ccgaaccaga taagtgaaat ctagttccaa
     3721 actattttgt catttttaat tttcgtatta gcttacgacg ctacacccag ttcccatcta
     3781 ttttgtcact cttccctaaa taatccttaa aaactccatt tccacccctc ccagttccca
     3841 actattttgt ccgcccacag cggggcattt ttcttcctgt tatgttttta atcaaacatc
     3901 ctgccaactc catgtgacaa accgtcatct tcggctactt tttctctgtc acagaatgaa
     3961 aatttttctg tcatctcttc gttattaatg tttgtaattg actgaatatc aacgcttatt
     4021 tgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4081 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4141 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4201 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4261 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4321 gagtccacgt tcttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4381 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     4441 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     4501 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     4561 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     4621 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     4681 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     4741 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     4801 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     4861 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     4921 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     4981 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     5041 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     5101 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     5161 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     5221 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     5281 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     5341 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     5401 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     5461 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     5521 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     5581 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     5641 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     5701 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     5761 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     5821 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     5881 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     5941 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     6001 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6061 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6121 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6181 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     6241 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6301 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     6361 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     6421 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     6481 catagaccag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa tggatgccct
     6541 gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga tctaaactat
     6601 gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc agttatgctg
     6661 tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt ctgaagtgca
     6721 aattgcccgt cgtattaaag aggggcgtgg ccaagggcat ggtaaagact atattcgcgg
     6781 cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat
     6841 tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac
     6901 tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa
     6961 gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc
     7021 ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca
     7081 aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca
     7141 agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt
     7201 tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg
     7261 gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct
     7321 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa
     7381 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa
     7441 aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc
     7501 aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt acgaaccgaa
     7561 caggcttatg tcaactgggt tcgtgccttc atccgtttcc acggtgtgcg tcacccggca
     7621 accttgggca gcagcgaagt cgaggcattt ctgtcctggc tggcgaacga gcgcaaggtt
     7681 tcggtctcca cgcatcgtca ggcattggcg gccttgctgt tcttctacgg caaggtgctg
     7741 tgcacggatc tgccctggct tcaggagatc ggaagacctc ggccgt
//
