LOCUS       sequence-221662-        6372 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 440, Emission: 620.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6372)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 6372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKeima-Red-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..6372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      574..583
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2304..2313
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2310..2312,2313..2315,2316..2978)
                     /codon_start=1
                     /product="monomeric Keima-Red fluorescent protein, also 
                     known as mKeima"
                     /label=mKeima-Red
                     /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV
                     TKGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTV
                     SNDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK
                     LEGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG
                     "
     polyA_signal    3100..3221
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3137..3156)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3191..3210
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3228..3683)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3365..3386)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3577..3596
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3710..3814
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3816..4173
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3816..4007
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3839..3859)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4024..4159
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4208..5002
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4208..5002
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4262..4281)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4872..4891
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5190..5209)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5234..5281
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5610..6198
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6099..6118
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagct caagcttcga attccatggc ggccccccgc ccgtctcccg
      601 cgatccccgt ttcggtctcg gctccggctt tttacgcccc gcagaagaag ttcggccctg
      661 tggtggcccc aaagcccaaa gtgaatccct tccggcccag ggacagcgag cctcccccgg
      721 cacccggggc ccagcgcgca cagatgggcc gggtgggcga tattcccccg ccgcccccgg
      781 aagactttcc cctgcctcca cctccccttg ctggggatgg cgacgatgca gagggtgctc
      841 tgggaggtgc cttcccgccg ccccctcccc cgatcgagga atcatttccc cctgcgcctc
      901 tggaggagga gatcttccct tccccgccgc ctcctccgga ggaggaggga gggcctgagg
      961 cccccatacc gcccccacca cagcccaggg agaaggtgag cagtattgat ttggagatcg
     1021 actctctgtc ctcactgctg gatgacatga ccaagaatga tcctttcaaa gcccgggtgt
     1081 catctggata tgtgccccca ccagtggcca ctccattcag ttccaagtcc agtaccaagc
     1141 ctgcagccgg gggcacagca cccctgcctc cttggaagtc cccttccagc tcccagcctc
     1201 tgccccaggt tccggctccg gctcagagcc agacacagtt ccatgttcag ccccagcccc
     1261 agcccaagcc tcaggtccaa ctccatgtcc agtcccagac ccagcctgtg tctttggcta
     1321 acacccagcc ccgagggccc ccagcctcat ctccggctcc agcccctaag ttttctccag
     1381 tgactcctaa gtttactcct gtggcttcca agttcagtcc tggagcccca ggtggatctg
     1441 ggtcacaacc aaatcaaaaa ttggggcacc ccgaagctct ttctgctggc acaggctccc
     1501 ctcaacctcc cagcttcacc tatgcccagc agagggagaa gccccgagtg caggagaagc
     1561 agcaccccgt gcccccaccg gctcagaacc aaaaccaggt gcgctcccct ggggccccag
     1621 ggcccctgac tctgaaggag gtggaggagc tggagcagct gacccagcag ctaatgcagg
     1681 acatggagca tcctcagagg cagaatgtgg ctgtcaacga actctgcggc cgatgccatc
     1741 aacccctggc ccgggcgcag ccagccgtcc gcgctctagg gcagctgttc cacatcgcct
     1801 gcttcacctg ccaccagtgt gcgcagcagc tccagggcca gcagttctac agtctggagg
     1861 gggcgccgta ctgcgagggc tgttacactg acaccctgga gaagtgtaac acctgcgggg
     1921 agcccatcac tgaccgcatg ctgagggcca cgggcaaggc ctatcacccg cactgcttca
     1981 cctgtgtggt ctgcgcccgc cccctggagg gcacctcctt catcgtggac caggccaacc
     2041 ggccccactg tgtccccgac taccacaagc agtacgcccc gaggtgctcc gtctgctctg
     2101 agcccatcat gcctgagcct ggccgagatg agactgtgcg agtggtcgcc ctggacaaga
     2161 acttccacat gaagtgttac aagtgtgagg actgcgggaa gcccctgtcg attgaggcag
     2221 atgacaatgg ctgcttcccc ctggacggtc acgtgctctg tcggaagtgc cacactgcta
     2281 gagcccagac ggatccaccg gtcgccacca tggtgagtgt gatcgctaaa caaatgacct
     2341 acaaggttta tatgtcaggc acggtcaatg gacactactt tgaggtcgaa ggcgatggaa
     2401 aaggaaagcc ttacgaggga gagcagacag taaagctcac tgtcaccaag ggtggacctc
     2461 tgccatttgc ttgggatatt ttatcaccac agcttcagta cggaagcata ccattcacca
     2521 agtaccctga agacatccct gattatttca agcagtcatt ccctgaggga tatacatggg
     2581 agaggagcat gaactttgaa gatggtgcag tgtgtactgt cagcaatgat tccagcatcc
     2641 aaggcaactg tttcatctac aatgtcaaaa tctctggtga gaactttcct cccaatggac
     2701 ctgttatgca gaagaagaca cagggctggg aacccagcac tgagcgtctc tttgcacgag
     2761 atggaatgct gataggaaac gattatatgg ctctgaagtt ggaaggaggt ggtcactatt
     2821 tgtgtgaatt taaatctact tacaaggcaa agaagcctgt gaggatgcca gggcgccacg
     2881 agattgaccg caaactggat gtaaccagtc acaacaggga ttacacatct gttgagcagt
     2941 gtgaaatagc cattgcacgc cactctttgc tcggttaagc ggccgcgact ctagatcata
     3001 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     3061 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     3121 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     3181 cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt
     3241 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     3301 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     3361 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     3421 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     3481 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     3541 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     3601 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     3661 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     3721 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     3781 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg
     3841 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3901 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     3961 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     4021 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4081 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     4141 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc
     4201 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     4261 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     4321 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     4381 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     4441 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     4501 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     4561 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     4621 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     4681 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
     4741 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     4801 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     4861 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     4921 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     4981 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     5041 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     5101 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     5161 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga
     5221 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
     5281 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     5341 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     5401 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca
     5461 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     5521 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5581 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5641 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5701 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     5761 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5821 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5881 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5941 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6001 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6061 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6121 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6181 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6241 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     6301 accgccatgc attagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     6361 tatggagttc cg
//
