LOCUS       sequence-221666-        5994 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CH192: PIq:luxR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Chen Y, Ho JML, Shis DL, Gupta C, Long J, Wagner DS, Ott W, Josic K,
            Bennett MR
  TITLE     Tuning the dynamic range of bacterial promoters regulated by 
            ligand-inducible transcription factors.
  JOURNAL   Nat Commun. 2018 Jan 4;9(1):64. doi: 10.1038/s41467-017-02473-5.
  PUBMED    29302024
REFERENCE   2  (bases 1 to 5994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-01-4"; volume: "9(1):64. doi"; pages: " 
            10.1038/s41467-017-02473-5"
COMMENT     Sequence Label: CH192
FEATURES             Location/Qualifiers
     source          1..5994
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..34
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      98..125
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(736..1395)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1310..1329
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1396..1498)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    1555..1588
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      complement(1725..1752)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(2905..2934)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3125..3147)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3136..3152
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3300..3531
                     /gene="attP"
                     /label=lambda attP
                     /bound_moiety="lambda integrase"
                     /note="integrase from phage lambda"
     terminator      3566..3652
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(4190..5140)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      5188..5410
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(5912..5946)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gaagttccta ttctctagaa agtataggaa cttcaacgcc atgagcggcc tcatttctta
       61 ttctgagtta caacagtccg caccgctgtc tcacactggc tcaccttcgg gtgggccttt
      121 ctgcgtttat atactagaga gagaatataa aaagccagat tattaatccg gcttttttat
      181 tatttcctag cggccgcagg cggtagctcc ttccggtggg cgcggggcat gactatcgtc
      241 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgccc
      301 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gccctgcacc
      361 attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct
      421 gtattaacga agcgctaacc gtttttatca ggctctggga ggcagaataa atgatcatat
      481 cgtcaattat tacctccacg gggagagcct gagcaaactg gcctcaggca tttgagaagc
      541 acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag acataagcgg
      601 ctatttaacg accctgccct gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc
      661 attcatccgc ttattatcac ttattcaggc gtagcaacca ggcgtttaag ggcaccaata
      721 actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
      781 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
      841 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
      901 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
      961 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     1021 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     1081 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     1141 ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag
     1201 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     1261 taaaaaggcc gtaatatcca gctgaacagt ctggttatag gtacattgag caactgactg
     1321 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     1381 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
     1441 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac
     1501 gtctcatttt cgccaaaagt tggcccaggg cttcccggta tcaacaggga caccgaagtt
     1561 cctattctct agaaagtata ggaacttctg ctcatgtttg acagcttatc atcgatgcat
     1621 aatgtgcctg tcaaatggac gaagcaggga ttctgctagc taggaaataa taaaaaagcc
     1681 ggattaataa tctggctttt tatattctct ctctagtata taaacgcaga aaggcccacc
     1741 cgaaggtgag ccagtgtgag acagcggtgc ggactgttgt aactcagaat aagaaatgag
     1801 gccgctcatg gcgttgactc tcagtcatag tatcgtggta tcaccggttg gttccactct
     1861 ctgttgcggg caacttcagc agcacgtagg ggaagcttat taatttttaa agtatgggca
     1921 atcaattgct cctgttaaaa ttgctttaga aatactttgg cagcggtttg ttgtattgag
     1981 tttcatttgc gcattggtta aatggaaagt gacagtacgc tcactgcagc ctaatatttt
     2041 tgaaatatcc caagagcttt ttccttcgca ggcccacgct aaacattctt tttctctttt
     2101 ggttaaatcg ttgtttgatt tattatttgc tatatttatt tttcgataat tatcaactag
     2161 agaaggaaca attaatggta tgttcataca cgcatgtaaa aataaactat ctatatagtt
     2221 gtctttttct gaatgtgcaa aactaagcat tccgaagcca ttgttagccg tatgaatagg
     2281 gaaactaaac ccagtgataa gacctgatgt tttcgcttct ttaattacat ttggagattt
     2341 tttatttaca gcattgtttt caaatatatt ccaattaatt ggtgaatgat tggagttaga
     2401 ataatctact ataggatcat attttattaa attagcgtca tcataatatt gcctccattt
     2461 tttagggtaa ttatctagaa ttgaaatatc agatttaacc atagaatgag gataaatgat
     2521 cgcgagtaaa taatattcac aatgtaccat tttagtcata tcagataagc attgattaat
     2581 atcattattg cttctacatg ctttaatttt attaattatt ctgtatgtgt cgtcggcatt
     2641 tatgtttttc atactagaat ttctccttag catgattaag atgtttcagt acgaaaattg
     2701 ctttcattgt tgatctcctt tttaagtggc atgcttccgg gcgctatcat gccataccgc
     2761 gaaaggtttt gcaccatcga cagttcatag gtgattgctc aggacatttc tgttagaagg
     2821 aatcgttttc cttacttttc cttacgcaca agagttccgt agctgttcaa gtttgtgttt
     2881 caactgttct cgtcgtttcc gcaacaagtc ctcttcagaa atgagctttt gctcctctgc
     2941 ttggacggac aggatgtatg ctgtggcttt tttaaggata actaccttgg gggccttttc
     3001 attgttttcc aactccggga tctggtcacg cagggcaaaa aagctccgtt ttagctcgtt
     3061 cctcctctgg cgctccaaga cgttgtgtgt tcgcctcttg acattctcct cggtgtccga
     3121 gggccctgtg tgaaattgtt atccgctcac aattccacac agacgtcgga attgccagct
     3181 ggggcgccct ctggtaaggt tgggaagctc gagcgcactg agctcctaga gtgcgcgaca
     3241 ggtttgatga caaaaaatta gcgcaagaag acaaaaatca ccttgcgcta atgctctgtt
     3301 acaggtcact aataccatct aagtagttga ttcatagtga ctgcatatgt tgtgttttac
     3361 agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat
     3421 tttacgtttc tcgttcagct tttttatact aagttggcat tataaaaaag cattgcttat
     3481 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgatttcaat
     3541 tttgtcccac tccccctaga ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     3601 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc
     3661 ctagacctag ggtacgggtt ttgctgcccg caaacgggct gttctggtgt tgctagtttg
     3721 ttatcagaat cgcagatccg gcttcagccg gtttgccggc tgaaagcgct atttcttcca
     3781 gaattgccat gattttttcc ccacgggagg cgtcactggc tcccgtgttg tcggcagctt
     3841 tgattcgata agcagcatcg cctgtttcag gctgtctatg tgtgactgtt gagctgtaac
     3901 aagttgtctc aggtgttcaa tttcatgttc tagttgcttt gttttactgg tttcacctgt
     3961 tctattaggt gttacatgct gttcatctgt tacattgtcg atctgttcat ggtgaacagc
     4021 tttgaatgca ccaaaaactc gtaaaagctc tgatgtatct atctttttta caccgttttc
     4081 atctgtgcat atggacagtt ttccctttga tatgtaacgg tgaacagttg ttctactttt
     4141 gtttgttagt cttgatgctt cactgataga tacaagagcc ataagaacct cagatccttc
     4201 cgtatttagc cagtatgttc tctagtgtgg ttcgttgttt ttgcgtgagc catgagaacg
     4261 aaccattgag atcatactta ctttgcatgt cactcaaaaa ttttgcctca aaactggtga
     4321 gctgaatttt tgcagttaaa gcatcgtgta gtgtttttct tagtccgtta tgtaggtagg
     4381 aatctgatgt aatggttgtt ggtattttgt caccattcat ttttatctgg ttgttctcaa
     4441 gttcggttac gagatccatt tgtctatcta gttcaacttg gaaaatcaac gtatcagtcg
     4501 ggcggcctcg cttatcaacc accaatttca tattgctgta agtgtttaaa tctttactta
     4561 ttggtttcaa aacccattgg ttaagccttt taaactcatg gtagttattt tcaagcatta
     4621 acatgaactt aaattcatca aggctaatct ctatatttgc cttgtgagtt ttcttttgtg
     4681 ttagttcttt taataaccac tcataaatcc tcatagagta tttgttttca aaagacttaa
     4741 catgttccag attatatttt atgaattttt ttaactggaa aagataaggc aatatctctt
     4801 cactaaaaac taattctaat ttttcgcttg agaacttggc atagtttgtc cactggaaaa
     4861 tctcaaagcc tttaaccaaa ggattcctga tttccacagt tctcgtcatc agctctctgg
     4921 ttgctttagc taatacacca taagcatttt ccctactgat gttcatcatc tgagcgtatt
     4981 ggttataagt gaacgatacc gtccgttctt tccttgtagg gttttcaatc gtggggttga
     5041 gtagtgccac acagcataaa attagcttgg tttcatgctc cgttaagtca tagcgactaa
     5101 tcgctagttc atttgctttg aaaacaacta attcagacat acatctcaat tggtctaggt
     5161 gattttaatc actataccaa ttgagatggg ctagtcaatg ataattacta gtccttttcc
     5221 tttgagttgt gggtatctgt aaattctgct agacctttgc tggaaaactt gtaaattctg
     5281 ctagaccctc tgtaaattcc gctagacctt tgtgtgtttt ttttgtttat attcaagtgg
     5341 ttataattta tagaataaag aaagaataaa aaaagataaa aagaatagat cccagccctg
     5401 tgtataactc actactttag tcagttccgc agtattacaa aaggatgtcg caaacgctgt
     5461 ttgctcctct acaaaacaga ccttaaaacc ctaaaggctt aagtagcacc ctcgcaagct
     5521 cgggcaaatc gctgaatatt ccttttgtct ccgaccatca ggcacctgag tcgctgtctt
     5581 tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag tgaatggggg taaatggcac
     5641 tacaggcgcc ttttatggat tcatgcaagg aaactaccca taatacaaga aaagcccgtc
     5701 acgggcttct cagggcgttt tatggcgggt ctgctatgtg gtgctatctg actttttgct
     5761 gttcagcagt tcctgccctc tgattttcca gtctgaccac ttcggattat cccgtgacag
     5821 gtcattcaga ctggctaatg cacccagtaa ggcagcggta tcatcaacag gcttacccgt
     5881 cttactgtcc ctagtgcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt
     5941 tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca ggag
//
