LOCUS       sequence-221706-       11240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  stable lentiviral expression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11240)
  AUTHORS   Liang JR, Lingeman E, Ahmed S, Corn JE
  TITLE     Atlastins remodel the endoplasmic reticulum for selective autophagy.
  JOURNAL   J Cell Biol. 2018 Aug 24. pii: jcb.201804185. doi: 
            10.1083/jcb.201804185.
  PUBMED    30143524
REFERENCE   2  (bases 1 to 11240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2018 Aug 24. pii: jcb.201804185. doi: 10.1083/jcb.201804185."
COMMENT     Sequence Label: pLenti-X1-Neo-GFP-FAM134B-LIRmut
FEATURES             Location/Qualifiers
     source          1..11240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        1196..1214
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1259..1261,1262..1264,1265..1975)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1259..1261,1262..1264,1265..1975)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1304..1325)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1565..1584)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1912..1933
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3499..3523)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3554..4142
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3607..3627)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4025..4036)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4193..4692
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4228..4251)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4625..4644
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4706..5509
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4769..4788)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5379..5398
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             5653..5886
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5958..6079
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5995..6014)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6049..6068
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6119..6254
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6275..6293)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6303..6320)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6303..6319)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6312..6334)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6461..6916
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6548..6567)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6758..6779
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6942..7046
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7047..7115,7116..7907)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7265..7284)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8078..8666
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8567..8586
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8820..8837
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8954..8975
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8990..8995,8996..9013,9014..9020)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9028..9044
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9033..9055
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9052..9068
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9052..9068
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9087..9107
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9089..9107
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9135..9361
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9362..9542
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9589..9714
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10207..10440
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10625..10669
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10936..11052
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    11086..11110
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata
     1201 cgactcacta tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccat
     1261 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1321 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1381 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1441 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     1501 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1561 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1621 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1681 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     1741 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     1801 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1861 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1921 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggtgg
     1981 atctggaggt tctggtggaa tggcgagccc ggcgcctccg gagcacgccg aggagggatg
     2041 cccggctcct gccgccgagg agcaggcgcc gccgtcgccg ccaccgcccc aggcatcccc
     2101 cgcagagcgg cagcagcagg aggaggaagc gcaggaagct ggggcggcgg agggcgcggg
     2161 gttgcaggtg gaggaggccg cgggccgggc ggcggccgcg gtaacctggc tgctcgggga
     2221 gccggtgctg tggctgggct gccgcgccga cgagctgctg agctggaaga ggccgctgcg
     2281 gagcctgctc ggcttcgtcg ctgccaacct gctgttctgg ttccttgcat tgactccatg
     2341 gagagtatat cacctgattt ccgtcatgat acttgggcgt gttattatgc aaataataaa
     2401 ggatatggtt ttgtctagaa caagaggtgc acagttgtgg agaagcctca gtgaaagctg
     2461 ggaagttatc aattccaaac cagatgaaag acccaggctc agccactgta ttgcagaatc
     2521 atggatgaat ttcagcatat ttcttcaaga aatgtctctt tttaaacagc agagccctgg
     2581 caagttttgt ctcctggtct gtagtgtgtg cacatttttt acgatcttgg gaagttacat
     2641 tcctggggtt atactcagct atctactgtt actgtgtgca tttttgtgtc cattgtttaa
     2701 atgtaatgat attggacaaa aaatttacag caaaattaag tcagttctgc tgaaactgga
     2761 ttttggaatt ggagaatata ttaatcagaa gaaacgtgag agatctgaag cagataaaga
     2821 aaaaagtcac aaagatgaca gtgaattaga cttttcagct ctttgtccta agattagcct
     2881 cacggttgct gccaaagagt tatctgtgtc tgacacagac gtctcagagg tatcctggac
     2941 tgataatggg accttcaacc tttcagaagg atacactcca cagacagaca cttctgatga
     3001 tcttgaccga cccagtgagg aagttttctc tagagatctt tcagattttc catctctaga
     3061 aaatggcatg ggaacaaatg atgaagatga attaagcctt ggtttgccca ctgagctcaa
     3121 gagaaagaag gaacagttgg acagtggtca cagaccaagc aaagagacgc aatcagcagc
     3181 tggtctcacc cttcctctga acagtgacca aacctttcac ctgatgagca acctggctgg
     3241 ggatgttatc acagctgcag tgactgcagc tatcaaagac cagttagagg gtgtgcagca
     3301 agcactttct caggctgccc ccatcccaga agaggacaca gacactgaag aaggtgatga
     3361 cgctgaagca cttgaccagt cagagctgga tcaaattgag agtgaattgg gacttacaca
     3421 agaccaggaa gcagaagcac agcaaaataa gaagtcttca ggtttccttt caaatctgct
     3481 gggaggccat taatctagac ccagctttct tgtacaaagt ggttgatatc cagcacagtg
     3541 gcggccgctc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     3601 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     3661 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     3721 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     3781 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     3841 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     3901 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     3961 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     4021 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     4081 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     4141 gcctggacct gcaggattct gcagatatcc ggactagtga tctaattcta ccgggtaggg
     4201 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
     4261 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
     4321 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
     4381 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
     4441 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
     4501 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
     4561 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc
     4621 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
     4681 ccgggccttt cgacctgcag ccaatatggg atcggccatt gaacaagatg gattgcacgc
     4741 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     4801 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     4861 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     4921 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     4981 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     5041 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     5101 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     5161 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     5221 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     5281 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     5341 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     5401 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     5461 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa cgcgtcgccc
     5521 gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat ctcgagggcc
     5581 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     5641 aaaagggggg actggaaggg ctagctcact cccaacgaag acaagatctg ctttttgctt
     5701 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     5761 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     5821 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     5881 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     5941 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6001 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6061 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     6121 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6181 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     6241 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     6301 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     6361 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     6421 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     6481 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6541 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6601 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6661 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6721 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6781 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6841 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     6901 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6961 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     7021 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     7081 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     7141 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     7201 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     7261 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     7321 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     7381 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     7441 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     7501 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     7561 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     7621 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     7681 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     7741 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     7801 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     7861 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     7921 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     7981 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     8041 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     8101 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     8161 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     8221 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     8281 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     8341 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     8401 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     8461 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     8521 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     8581 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     8641 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     8701 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     8761 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     8821 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     8881 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     8941 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     9001 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     9061 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     9121 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     9181 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     9241 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     9301 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     9361 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     9421 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     9481 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     9541 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     9601 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     9661 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     9721 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     9781 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     9841 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     9901 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     9961 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
    10021 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
    10081 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
    10141 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
    10201 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
    10261 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
    10321 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
    10381 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
    10441 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
    10501 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
    10561 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
    10621 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
    10681 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
    10741 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
    10801 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
    10861 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
    10921 acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
    10981 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
    11041 attcaaaatt ttatcgtcca gtgtggtgga attctgcaga tatcaacaag tttgtacaaa
    11101 aaagcaggct ccaccatggg aaccgacatt gattattgac taggcttttg caaaaagctt
    11161 tgcaaagatg gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg
    11221 tcttgaaagg agtgggaatt
//
