LOCUS       sequence-221707-       11498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  stable lentiviral expression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11498)
  AUTHORS   Liang JR, Lingeman E, Ahmed S, Corn JE
  TITLE     Atlastins remodel the endoplasmic reticulum for selective autophagy.
  JOURNAL   J Cell Biol. 2018 Aug 24. pii: jcb.201804185. doi: 
            10.1083/jcb.201804185.
  PUBMED    30143524
REFERENCE   2  (bases 1 to 11498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2018 Aug 24. pii: jcb.201804185. doi: 10.1083/jcb.201804185."
COMMENT     Sequence Label: pLenti-X1-Neo-GFP-ATL2-R244Q
FEATURES             Location/Qualifiers
     source          1..11498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        1196..1214
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1259..1261,1262..1264,1265..1975)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1259..1261,1262..1264,1265..1975)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1304..1325)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1565..1584)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1912..1933
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3757..3781)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3812..4400
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3865..3885)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4283..4294)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4451..4950
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4486..4509)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4883..4902
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4964..5767
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5027..5046)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5637..5656
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             5911..6144
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6216..6337
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6253..6272)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6307..6326
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6377..6512
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6533..6551)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6561..6578)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6561..6577)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6570..6592)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6719..7174
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6806..6825)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7016..7037
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7200..7304
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7305..7373,7374..8165)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7523..7542)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8336..8924
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8825..8844
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9078..9095
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9212..9233
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9248..9253,9254..9271,9272..9278)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9286..9302
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9291..9313
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9310..9326
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9310..9326
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9345..9365
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9347..9365
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9393..9619
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9620..9800
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9847..9972
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10465..10698
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10883..10927
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11194..11310
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    11344..11368
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata
     1201 cgactcacta tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccat
     1261 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1321 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1381 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1441 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     1501 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1561 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1621 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1681 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     1741 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     1801 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1861 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1921 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggtgg
     1981 atctggaggt tctggtggaa tggcggaggg ggacgaggca gcgcgagggc agcaaccgca
     2041 ccaggggctg cggcgccggc gacggaccag cgacccaagc gccgcggtta accacgtctc
     2101 gtccacgacc tccctaggtg agaattatga agatgatgac ctagtaaatt ctgatgaggt
     2161 tatgaagaaa ccatgtccag tacagattgt tcttgctcat gaagatgacc ataactttga
     2221 acttgatgaa gaagctttgg agcagatatt gctacaggag cacatacgag atcttaacat
     2281 agtagtggta tctgtggcag gagcttttcg taaagggaag tcatttctac tggacttcat
     2341 gcttagatac atgtataata aggattctca aagttggatt ggtggaaaca atgaaccatt
     2401 gacaggcttt acatggcgag gtggctgtga aagagaaaca acaggcatac aagtttggaa
     2461 tgaagtattt gtgattgaca gacctaatgg aactaaagtt gctgtgctgc ttatggatac
     2521 ccagggtgcc tttgatagcc agtcaactat caaagactgt gcaacggtgt ttgctctgag
     2581 cactatgact agctctgtcc aggtatataa tctgtctcag aatattcaag aagatgatct
     2641 tcaacatttg caattattta cagagtatgg aagacttgcg atggaagaaa tctaccagaa
     2701 accatttcag acattaatgt ttttgattca agattggagc tatccttatg aacattcata
     2761 tggtttggaa ggtggaaagc aatttcttga aaagagatta caggtaaaac aaaatcaaca
     2821 tgaagagctt cagaatgtaa ggaagcacat acacaattgt ttctcaaatc ttggttgctt
     2881 ccttttgcca catcctggtc ttaaagttgc aactaatcct agttttgatg ggagattgaa
     2941 agatattgat gaagacttta aacgcgagct tcgaaatctg gttccattgc tgcttgcccc
     3001 tgaaaatttg gtagaaaaag agataagtgg atctaaagtc acttgtagag atcttgtaga
     3061 atattttaag gcttacatca aaatctatca aggagaagaa cttccacatc caaagtccat
     3121 gcttcaggca acagctgaag ctaataatct tgctgcagta gcaggagcaa gagataccta
     3181 ttgtaaaagt atggaacagg tatgtggagg ggacaagcct tacattgcac cttcagatct
     3241 ggagcgaaaa cacttggatc tcaaggaagt ggcgataaaa caatttcgtt cagtaaaaaa
     3301 gatgggtgga gatgagttct gccgtcgtta tcaggaccag cttgaagctg aaattgaaga
     3361 aacctatgca aattttataa agcacaatga tggcaaaaat atcttctatg ctgctcgtac
     3421 cccagccaca ctgtttgcgg tcatgtttgc tatgtatata atctcaggac tgactggctt
     3481 cattggccta aactctatag ctgtcttgtg taaccttgtc atggggttag cactgatatt
     3541 tctttgtact tgggcatatg ttaaatactc tggggagttc agagaaattg gaacagtgat
     3601 tgatcagatt gctgaaacac tatgggaaca ggtattgaag cccctgggtg ataatttgat
     3661 ggaggaaaac ataaggcagt ctgtaacaaa ctctatcaaa gcaggcctga ctgaccaggt
     3721 gtctcatcat gccagattaa agacagactg atctagaccc agctttcttg tacaaagtgg
     3781 ttgatatcca gcacagtggc ggccgctcga caatcaacct ctggattaca aaatttgtga
     3841 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     3901 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     3961 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     4021 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4081 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     4141 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     4201 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     4261 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     4321 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     4381 cctttgggcc gcctccccgc ctggacctgc aggattctgc agatatccgg actagtgatc
     4441 taattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
     4501 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
     4561 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
     4621 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
     4681 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
     4741 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
     4801 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
     4861 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
     4921 ttctcctctt cctcatctcc gggcctttcg acctgcagcc aatatgggat cggccattga
     4981 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     5041 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     5101 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     5161 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     5221 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     5281 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     5341 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     5401 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     5461 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     5521 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     5581 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     5641 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     5701 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     5761 cttctgaacg cgtcgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc
     5821 ccatgcatct cgagggcccg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     5881 ttagccactt tttaaaagaa aaggggggac tggaagggct agctcactcc caacgaagac
     5941 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     6001 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6061 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6121 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     6181 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     6241 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6301 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     6361 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6421 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     6481 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     6541 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     6601 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     6661 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     6721 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     6781 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     6841 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     6901 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     6961 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     7021 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     7081 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     7141 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     7201 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     7261 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     7321 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     7381 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     7441 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     7501 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     7561 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     7621 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     7681 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     7741 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7801 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     7861 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7921 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7981 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     8041 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     8101 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     8161 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     8221 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     8281 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     8341 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     8401 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     8461 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     8521 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     8581 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8641 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8701 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8761 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8821 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8881 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8941 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     9001 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     9061 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     9121 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     9181 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     9241 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     9301 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     9361 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     9421 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     9481 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     9541 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     9601 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     9661 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9721 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9781 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     9841 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     9901 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     9961 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
    10021 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
    10081 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
    10141 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
    10201 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
    10261 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
    10321 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
    10381 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
    10441 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
    10501 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
    10561 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
    10621 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
    10681 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
    10741 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
    10801 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
    10861 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
    10921 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
    10981 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
    11041 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
    11101 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
    11161 attagtgaac ggatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg
    11221 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
    11281 acaaaaacaa attacaaaat tcaaaatttt atcgtccagt gtggtggaat tctgcagata
    11341 tcaacaagtt tgtacaaaaa agcaggctcc accatgggaa ccgacattga ttattgacta
    11401 ggcttttgca aaaagctttg caaagatgga taaagtttta aacagagagg aatctttgca
    11461 gctaatggac cttctaggtc ttgaaaggag tgggaatt
//
