LOCUS       sequence-221714-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Light activated recruitment to OMM in mammalian cells with Cry2/CIB 
            optogenetic switch.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Godwin WC, Hoffmann GF, Gray TJ, Hughes RM
  TITLE     Imaging of morphological and biochemical hallmarks of apoptosis with
            optimized optogenetic tools.
  JOURNAL   J Biol Chem. 2019 Nov 8;294(45):16918-16929. doi: 
            10.1074/jbc.RA119.009141. Epub 2019 Oct 3.
  PUBMED    31582560
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA119.009141"; journalName: "J Biol Chem"; date: 
            "2019-11-8- 8"; volume: "294"; issue: "45"; pages: "16918-16929"
COMMENT     Sequence Label: Tom20-CIB-GFP
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             226..735
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
ORIGIN
        1 atggtgggcc ggaacagcgc tattgccgct ggagtgtgcg gcgccctgtt catcggctac
       61 tgcatctact tcgacaggaa gagaaggagc gaccccaact tcaagaacag gctgagggag
      121 agaaggaaga agcagaagct ggccaaggag agagccggcc tgagcaagct gcccgacctg
      181 aaggacgccg aggccgtgca gaagttcggc ggcagcggcg gcagcatgaa tggagctata
      241 ggaggtgacc ttttgctcaa ttttcctgac atgtcggtcc tagagcgcca aagggctcac
      301 ctcaagtacc tcaatcccac ctttgattct cctctcgccg gcttctttgc cgattcttca
      361 atgattaccg gcggcgagat ggacagctat ctttcgactg ccggtttgaa tcttccgatg
      421 atgtacggtg agacgacggt ggaaggtgat tcaagactct caatttcgcc ggaaacgacg
      481 cttgggactg gaaatttcaa ggcagcgaag tttgatacag agactaagga ttgtaatgag
      541 gcggcgaaga agatgacgat gaacagagat gacctagtag aagaaggaga agaagagaag
      601 tcgaaaataa cagagcaaaa caatgggagc acaaaaagca tcaagaagat gaaacacaaa
      661 gccaagaaag aagagaacaa tttctctaat gattcatcta aagtgacgaa ggaattggag
      721 aaaacggatt atattcatgg cggcagcggc ggcagccgaa gcttcgaatt c
//
