LOCUS       sequence-221715-        3413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL70005
FEATURES             Location/Qualifiers
     source          1..3413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        915..1093
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        947..1129
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      1729..1981
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2040..2058)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2126..2230
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2452..3243
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaggag
      841 cggagaatta agggagtcac gttatgaccc ccgccgatga cgcgggacaa gccgttttac
      901 gtttggaact gacagaaccg caacgttgaa ggagccactc agccgcgggt ttctggagtt
      961 taatgagcta agcacatacg tcagaaacca ttattgcgcg ttcaaaagtc gcctaaggtc
     1021 actatcagct agcaaatatt tcttgtcaaa atgctccact gacgttccat aaattcccct
     1081 cggtatccaa ttagagtctc atattcactc tcaatccaaa taatctgcac cggatctgga
     1141 tcgtttcgca tgtctcctga aagaaggcct gctgatatta gaagggctac tgaggctgat
     1201 atgcctgctg tgtgcactat tgtgaatcat tacattgaga cttctactgt gaatttcagg
     1261 actgagcctc aagaaccaca agagtggact gatgatcttg tgaggcttag agagaggtat
     1321 ccttggcttg ttgctgaagt ggatggtgaa gtggctggta ttgcttatgc tggtccttgg
     1381 aaggctagga atgcttacga ttggactgct gagtctactg tttacgtgtc tcctaggcat
     1441 caaagaactg gtcttggatc tactctttac actcatcttt tgaagtcttt ggaggctcag
     1501 ggtttcaagt ctgtggttgc tgtgattgga cttcctaatg atccttctgt gaggatgcat
     1561 gaggctcttg gttatgctcc taggggaatg cttagagctg ctggtttcaa acatggaaat
     1621 tggcatgatg tgggattctg gcagcttgat ttctctcttc ctgttcctcc tagacctgtt
     1681 cttcctgtga ctgagatttg agcgggactc tggatctaga gtcaagcaga tcgttcaaac
     1741 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
     1801 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
     1861 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
     1921 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat
     1981 cgacgcttgc tgaattggag cgcttgagac cacgaagtgg ctcttcagtg gacgaaaggg
     2041 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     2101 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     2161 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     2221 aaggaagagt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     2281 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttttggggt acagtctatg
     2341 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     2401 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa
     2461 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     2521 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     2581 ccacacagcg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     2641 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     2701 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     2761 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     2821 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     2881 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     2941 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     3001 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     3061 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     3121 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     3181 gatcagttgg aagaatttgt ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa
     3241 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     3301 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     3361 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttc
//
