LOCUS       sequence-221756-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human Cdt1 fused to HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Tsanov N, Kermi C, Coulombe P, Van der Laan S, Hodroj D, Maiorano D
  TITLE     PIP degron proteins, substrates of CRL4Cdt2, and not PIP boxes, 
            interfere with DNA polymerase eta and kappa focus formation on UV 
            damage.
  JOURNAL   Nucleic Acids Res. 2014 Apr;42(6):3692-706. doi: 
            10.1093/nar/gkt1400. Epub 2014 Jan 14.
  PUBMED    24423875
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkt1400"; journalName: "Nucleic Acids Res"; date: 
            "2014-04"; volume: "42"; issue: "6"; pages: "3692-706"
COMMENT     Sequence Label: pcDNA3-hCdt1-HA
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             83..355
                     /codon_start=1
                     /product="degron consisting of residues 30-120 of human 
                     Cdt1 (Zielke and Edgar, 2015)"
                     /label=Cdt1 (30-120)
                     /translation="PSPARPALRAPASATSGSRKRARPPAAPGRDQARPPARRRLRLSV
                     DEVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTI"
ORIGIN
        1 agcgccgcgt caccgacttc ttcgcggcgc cgccgccccg ggcccccccg catcggcgcc
       61 gcccaagctg gcctgccgca cccccagccc cgccaggccc gcactccgcg ccccggcctc
      121 cgctaccagt ggcagccgca agcgcgcccg cccgcccgcc gcccccggac gcgaccaggc
      181 caggccaccg gcccgcagga gactgcggct gtcggtggac gaggtttcca gccccagtac
      241 ccccgaggcc ccagacatcc cagcctgccc ttctccgggc cagaagataa agaaatccac
      301 cccggcagca ggtcagccgc cccacctgac atccgcgcag gaccaggaca ccatctctga
      361 gcttgcgtca tgcctgcaac gggcccggga gctgggggca agagtccggg cgctgaaggc
      421 cagtgcccag gatgctgggg agtcctgcac cccagaggcc gagggccgcc ctgaggagcc
      481 atgtggcgag aaggcgcccg cctaccagcg cttccatgcc ctggcccagc ccggcctgcc
      541 gggactcgtg ctgccctaca agtaccaggt gctggcggag atgttccgca gcatggacac
      601 catcgtgggc atgctccaca accgctccga gacgcccacc tttgccaagg tccagcgggg
      661 cgtccaggac atgatgcgta ggcgttttga ggagcgcaat gttggccaga tcaaaaccgt
      721 gtacccggcc tcctacccgc ttccgccagg agcgcagtgt cctccacctt caaggatggc
      781 accaggaggt cagattacca gctcaccatc gagccactgc tggagcagga ggctgacgga
      841 gcagcc
//
