LOCUS       sequence-221758-         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human Cdt1 fused to HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Tsanov N, Kermi C, Coulombe P, Van der Laan S, Hodroj D, Maiorano D
  TITLE     PIP degron proteins, substrates of CRL4Cdt2, and not PIP boxes, 
            interfere with DNA polymerase eta and kappa focus formation on UV 
            damage.
  JOURNAL   Nucleic Acids Res. 2014 Apr;42(6):3692-706. doi: 
            10.1093/nar/gkt1400. Epub 2014 Jan 14.
  PUBMED    24423875
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkt1400"; journalName: "Nucleic Acids Res"; date: 
            "2014-04"; volume: "42"; issue: "6"; pages: "3692-706"
COMMENT     Sequence Label: pcDNA3-hCdt1-HA
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             499..525
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     promoter        complement(544..562)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(545..562)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(582..599)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    588..699
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ggcctgcgct ctgctgcgcc cagcagcccc gggtctccca ggccagcact gccggctacc
       61 ccaccagcca ccccgcctgc agcctctccc agtgctctga agggggtgtc ccaggatctg
      121 ctggagcgga tccgagccaa ggaggcacag aagcagctgg cacagatgac gcggtgcccg
      181 gagcaggagc agcggctgca gcgcttagaa cggctgcctg agctggcccg cgtgctgcgg
      241 agcgtctttg tgtccgaacg caagcctgcg ctcagcatgg aggtggcctg tgccaggatg
      301 gtgggcagct gttgtactat catgagccct ggggaaatgg agaagcacct gctgctcctc
      361 tccgagctgc tgccggactg gctcagcctc caccgcatcc gcaccgacac ctacgtcaag
      421 ctggacaagg ccgcggacct cgcccacatc actgcacgcc tggcccacca gacacgtgct
      481 gaggaggggc tgaattctta tgatgttcct gattatgcta gcctctaatc tagagggccc
      541 tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg tgccttctag
      601 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
      661 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
      721 ttctattctg gggggtgggg tggggcagac a
//
