LOCUS       sequence-221761-       19616 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  binary vector for ABE expression in Arabidopsis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19616)
  AUTHORS   Kang BC, Yun JY, Kim ST, Shin Y, Ryu J, Choi M, Woo JW, Kim JS
  TITLE     Precision genome engineering through adenine base editing in plants.
  JOURNAL   Nat Plants. 2018 Jun 4. pii: 10.1038/s41477-018-0178-x. doi: 
            10.1038/s41477-018-0178-x.
  PUBMED    29867128
REFERENCE   2  (bases 1 to 19616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2018 Jun 4. pii: 10.1038/s41477-018-0178-x. doi: 
            10.1038/s41477-018-0178-x."
COMMENT     Sequence Label: pJY-RpABE
FEATURES             Location/Qualifiers
     source          1..19616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        243..318
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             join(3198..3221,3222..3224,3225..7298)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7311..7331
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7345..7593
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     protein_bind    complement(7602..7626)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9772..10467
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     terminator      10468..10719
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    10823..10847
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12178..12807
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             13236..14309
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      14375..14569
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(14805..14827)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    14913..15053
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     14968..14987
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(15068..15085)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15239..15827)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15319..15338)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(16073..16864)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    17385..17409
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(17502..17754)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(17952..18977)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(19013..19192)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     regulatory      19330..19339
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    19352..19376
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gaataaaaca tcttcattct taagatatga agataatctt caaaaggccc ctgggaatct
       61 gaaagaagag aagcaggccc atttatatgg gaaagaacaa tagtatttct tatataggcc
      121 catttaagtt gaaaacaatc ttcaaaagtc ccacatcgct tagataagaa aacgaagctg
      181 agtttatata cagctagagt cgaagtagtg attgaaatgc aggtgatgac tcacctgcat
      241 aagttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
      301 gtggcaccga gtcggtgctt tttttttttg cggccgcgat ccctcaactt ttgattcgct
      361 atttgcagtg cacctgtggc gttcatcaca tcttttgtga cactgtttgc actggtcatt
      421 gctattacaa aggaccttcc tgatgttgaa ggagatcgaa agtaagtaac tgcacgcata
      481 accattttct ttccgctctt tggctcaatc catttgacag tcaaagacaa tgtttaacca
      541 gctccgtttg atatattgtc tttatgtgtt tgttcaagca tgtttagtta atcatgcctt
      601 tgattgatct tgaataggtt ccaaatatca accctggcaa caaaacttgg agtgagaaac
      661 attgcattcc tcggttctgg acttctgcta gtaaattatg tttcagccat atcactagct
      721 ttctacatgc ctcaggtgaa ttcatctatt tccgtcttaa ctatttcggt taattaaagc
      781 acgaacacca ttactgcatg tagaagcttg ataaactatc gccaccaatt tatttttgtt
      841 gcgatattgt tactttcctc agtatgcagc tttgaaaaga ccaaccctct tatcctttaa
      901 caatgaacag gtttttagag gtagcttgat gattcctgca catgtgatct tggcttcagg
      961 cttaattttc caggtaaagc attatgagat actcttatat ctcttacata cttttgagat
     1021 aatgcacaag aacttcataa ctatatgctt tagtttctgc atttgacact gccaaattca
     1081 ttaatctcta atatctttgt tgttgatctt tggtagacat gggtactaga aaaagcaaac
     1141 tacaccaagg taaaatactt ttgtacaaac ataaactcgt tatcacggaa catcaatgga
     1201 gtgtatatct aacggagtgt agaaacattt gattattgca ggaagctatc tcaggatatt
     1261 atcggtttat atggaatctc ttctacgcag agtatctgtt attccccttc ctctagcttt
     1321 caatttcatg gtgaggatat gcagttttct ttgtatatca ttcttcttct tctttgtagc
     1381 ttggagtcaa aatcggttcc ttcatgtaca tacatcaagg atatgtcctt ctgaattttt
     1441 atatcttgca ataaaaatgc ttgtaccaat tgaaacacca gctttttgag ttctatgatc
     1501 actgacttgg ttctaaccaa aaaaaaaaaa tgtttaattt acatatctaa aagtaggttt
     1561 agggaaacct aaacagtaaa atatttgtat attattcgaa tttcactcat cataaaaact
     1621 taaattgcac cataaaattt tgttttacta ttaatgatgt aatttgtgta acttaagata
     1681 aaaataatat tccgtaagtt aaccggctaa aaccacgtat aaaccaggga acctgttaaa
     1741 ccggttcttt actggataaa gaaatgaaag cccatgtaga cagctccatt agagcccaaa
     1801 ccctaaattt ctcatctata taaaaggagt gacattaggg tttttgttcg tcctcttaaa
     1861 gcttctcgtt ttctctgccg tctctctcat tcgcgcgacg caaacgatct tcaggtgatc
     1921 ttctttctcc aaatcctctc tcataactct gatttcgtac ttgtgtattt gagctcacgc
     1981 tctgtttctc tcaccacagc cccatgatga gcgaggtcga gtttagccat gaatactgga
     2041 tgagacatgc tcttacttta gcgaaacgtg cgtgggacga gagagaggtt ccggtgggcg
     2101 cggtgttagt acacaataac agggttatcg gcgaagggtg gaacaggcct attggcaggc
     2161 acgatccgac tgcacatgcg gagatcatgg cgctacgaca gggggggctc gtcatgcaga
     2221 attatcgatt gatagacgcc acgctgtacg tcactctgga accatgcgta atgtgtgcag
     2281 gagcgatgat ccactctaga attggaagag tagtcttcgg cgcgagggat gcaaagaccg
     2341 gggcggcggg gtccctaatg gacgtgcttc accaccctgg catgaaccat agagtggaga
     2401 tcaccgaggg catacttgct gatgaatgtg cagcacttct atccgatttc tttcgaatga
     2461 gaaggcagga aattaaagcc cagaagaagg ctcaatctag tacagactct ggagggtcca
     2521 gtggcggctc atccgggagc gaaacaccag ggacgtcaga atccgctacg cccgaatcta
     2581 gtggtggctc cagcggagga agtagtgagg tggagttctc tcacgaatac tggatgcgac
     2641 atgctctaac gctagcaaaa cgagcgaggg atgaacgaga ggttcctgta ggagcagtgt
     2701 tggttctgaa caacagagtt attggtgaag gttggaatcg tgctattggg cttcacgacc
     2761 caacagccca tgccgaaata atggcgctca ggcaaggagg cttagtaatg caaaactaca
     2821 gattaatcga cgcgaccctg tatgtcacgt tcgagccatg cgttatgtgc gcgggcgcga
     2881 tgattcattc tagaattgga agggttgttt ttggggtgag aaatgccaaa actggtgcgg
     2941 ctgggagtct tatggacgtt ctccactacc ctggtatgaa ccatcgagtg gaaatcacgg
     3001 aggggatttt ggcggacgaa tgtgcagcat tgttatgcta tttcttccgt atgcctaggc
     3061 aggtcttcaa cgctcagaag aaggcgcaaa gttcaaccga ttcagggggg tcttctggcg
     3121 gatcttctgg ttcagagacg cccggtacct cagaatcagc tacgcctgag agttcaggtg
     3181 ggtcttctgg cggcagcgac aagaagtaca gcatcggcct ggccatcggt accaacagcg
     3241 tgggctgggc cgtgatcacc gacgagtaca aggtgcccag caagaagttc aaggtgctgg
     3301 gcaacaccga ccgccacagc atcaagaaga acctgatcgg cgccctgctg ttcgacagcg
     3361 gcgagaccgc cgaggccacc cgcctgaagc gcaccgcccg ccgccgctac acccgccgca
     3421 agaaccgcat ctgctacctg caggagatct tcagcaacga gatggccaag gtggacgaca
     3481 gcttcttcca ccgcctggag gagagcttcc tggtggagga ggacaagaag cacgagcgcc
     3541 accccatctt cggcaacatc gtggacgagg tggcctacca cgagaagtac cccaccatct
     3601 accatctgcg caagaagctg gtggacagca ccgacaaggc cgacctgcgc ctgatctacc
     3661 tggccctggc ccacatgatc aagttccgcg gccacttcct gatcgagggc gacctgaacc
     3721 ccgacaacag cgacgtggac aagctgttca tccagctggt gcagacctac aaccagctgt
     3781 tcgaggagaa ccccatcaac gccagcggcg tggacgccaa ggccatcctg agcgcccgcc
     3841 tgagcaagag ccgccgcctg gagaacctga tcgcccagct gcccggcgag aagaagaacg
     3901 gcctgttcgg caacctgatc gccctgagcc tgggcctgac ccccaacttc aagagcaact
     3961 tcgacctggc cgaggacgcc aagctgcagc tgagcaagga cacctacgac gacgacctgg
     4021 acaacctgct ggcccagatc ggcgaccagt acgccgacct gttcctggcc gccaagaacc
     4081 tgagcgacgc catcctgctg agcgacatcc tgcgcgtgaa caccgagatc accaaggccc
     4141 ccctgagcgc cagcatgatc aagcgctacg acgagcacca ccaggacctg accctgctga
     4201 aggccctggt gcgccagcag ctgcccgaga agtacaagga gatcttcttc gaccagagca
     4261 agaacggcta cgccggctac atcgacggcg gcgccagcca ggaggagttc tacaagttca
     4321 tcaagcccat cctggagaag atggacggca ccgaggagct gctggtgaag ctgaaccgcg
     4381 aggacctgct gcgcaagcag cgcaccttcg acaacggcag catcccccac cagatccacc
     4441 tgggcgagct gcacgccatc ctgcgccgcc aggaggactt ctaccccttc ctgaaggaca
     4501 accgcgagaa gatcgagaag atcctgacct tccgcatccc ctactacgtg ggccccctgg
     4561 cccgcggcaa cagccgcttc gcctggatga cccgcaagag cgaggagacc atcaccccct
     4621 ggaacttcga ggaggtggtg gacaagggcg ccagcgccca gagcttcatc gagcgcatga
     4681 ccaacttcga caagaacctg cccaacgaga aggtgctgcc caagcacagc ctgctgtacg
     4741 agtacttcac cgtgtacaac gagctgacca aggtgaagta cgtgaccgag ggcatgcgca
     4801 agcccgcctt cctgagcggc gagcagaaga aggccatcgt ggacctgctg ttcaagacca
     4861 accgcaaggt gaccgtgaag cagctgaagg aggactactt caagaagatc gagtgcttcg
     4921 acagcgtgga gatcagcggc gtggaggacc gcttcaacgc cagcctgggc acctaccacg
     4981 acctgctgaa gatcatcaag gacaaggact tcctggacaa cgaggagaac gaggacatcc
     5041 tggaggacat cgtgctgacc ctgaccctgt tcgaggaccg cgagatgatc gaggagcgcc
     5101 tgaagaccta cgcccacctg ttcgacgaca aggtgatgaa gcagctgaag cgccgccgct
     5161 acaccggctg gggccgcctg agccgcaagc ttatcaacgg catccgcgac aagcagagcg
     5221 gcaagaccat cctggacttc ctgaagagcg acggcttcgc caaccgcaac ttcatgcagc
     5281 tgatccacga cgacagcctg accttcaagg aggacatcca gaaggcccag gtgagcggcc
     5341 agggcgacag cctgcacgag cacatcgcca acctggccgg cagccccgcc atcaagaagg
     5401 gcatcctgca gaccgtgaag gtggtggacg agctggtgaa ggtgatgggc cgccacaagc
     5461 ccgagaacat cgtgatcgag atggcccgcg agaaccagac cacccagaag ggccagaaga
     5521 acagccgcga gcgcatgaag cgcatcgagg agggcatcaa ggagctgggc agccagatcc
     5581 tgaaggagca ccccgtggag aacacccagc tgcagaacga gaagctgtac ctgtactacc
     5641 tgcagaacgg ccgcgacatg tacgtggacc aggagctgga catcaaccgc ctgagcgact
     5701 acgacgtgga ccacatcgtg ccccagagct tcctgaagga cgacagcatc gacaacaagg
     5761 tgctgacccg cagcgacaag aaccgcggca agagcgacaa cgtgcccagc gaggaggtgg
     5821 tgaagaagat gaagaactac tggcgccagc tgctgaacgc caagctgatc acccagcgca
     5881 agttcgacaa cctgaccaag gccgagcgcg gcggcctgag cgagctggac aaggccggct
     5941 tcatcaagcg ccagctggtg gagacccgcc agatcaccaa gcacgtggcc cagatcctgg
     6001 acagccgcat gaacaccaag tacgacgaga acgacaagct gatccgcgag gtgaaggtga
     6061 tcaccctgaa gagcaagctg gtgagcgact tccgcaagga cttccagttc tacaaggtgc
     6121 gcgagatcaa caactaccac cacgcccacg acgcctacct gaacgccgtg gtgggcaccg
     6181 ccctgatcaa gaagtacccc aagctggaga gcgagttcgt gtacggcgac tacaaggtgt
     6241 acgacgtgcg caagatgatc gccaagagcg agcaggagat cggcaaggcc accgccaagt
     6301 acttcttcta cagcaacatc atgaacttct tcaagaccga gatcaccctg gccaacggcg
     6361 agatccgcaa gcgccccctg atcgagacca acggcgagac cggcgagatc gtgtgggaca
     6421 agggccgcga cttcgccacc gtgcgcaagg tgctgagcat gccccaggtg aacatcgtga
     6481 agaagaccga ggtgcagacc ggcggcttca gcaaggagag catcctgccc aagcgcaaca
     6541 gcgacaagct gatcgcccgc aagaaggact gggaccccaa gaagtacggc ggcttcgaca
     6601 gccccaccgt ggcctacagc gtgctggtgg tggccaaggt ggagaagggc aagagcaaga
     6661 agctgaagag cgtgaaggag ctgctgggca tcaccatcat ggagcgcagc agcttcgaga
     6721 agaaccccat cgacttcctg gaggccaagg gctacaagga ggtgaagaag gacctgatca
     6781 tcaagctgcc caagtacagc ctgttcgagc tggagaacgg ccgcaagcgc atgctggcca
     6841 gcgccggcga gctgcagaag ggcaacgagc tggccctgcc cagcaagtac gtgaacttcc
     6901 tgtacctggc cagccactac gagaagctga agggcagccc cgaggacaac gagcagaagc
     6961 agctgttcgt ggagcagcac aagcactacc tggacgagat catcgagcag atcagcgagt
     7021 tcagcaagcg cgtgatcctg gccgacgcca acctggacaa ggtgctgagc gcctacaaca
     7081 agcaccgcga caagcccatc cgcgagcagg ccgagaacat catccacctg ttcaccctga
     7141 ccaacctggg cgcccccgcc gccttcaagt acttcgacac caccatcgac cgcaagcgct
     7201 acaccagcac caaggaggtg ctggacgcca ccctgatcca ccagagcatc accggtctgt
     7261 acgagacccg catcgacctg agccagctgg gcggcgactc tggtggttct ccaaagaaaa
     7321 agagaaaagt ataaaagggt gggatatgaa gatgaagatg aaatatttgg tgtgtcaaat
     7381 aaaaagcttg tgtgcttaag tttgtgtttt tttcttggct tgttgtgtta tgaatttgtg
     7441 gctttttcta atattaaatg aatgtaagat ctcattataa tgaataaaca aatgtttcta
     7501 taatccattg tgaatgtttt gttggatctc ttctgcagca tataactact gtatgtgcta
     7561 tggtatggac tatggaatat gattaaagat aaggcgcgcc gacccagctt tcttgtacaa
     7621 agtggtgata tcactagtgt atgtaggtat agtaacatga acaagaacag actcaagtac
     7681 aagatcgcat acgaaaatgg aaatggcaat gtcacttcca cataatcaaa cacgaatcct
     7741 catatcaaca aggcctgaga ttctaactag ctcataacaa cttagccaat agttacttga
     7801 gactaccaaa tgtatgtaga actaaagact aagggacaga gagttcgtct aaacaggtga
     7861 atctagtcgt tgttatctaa taaacaattc agccccaaat gcagaacaca catagagctc
     7921 tctattgatt caaattacga tctgatactg ataacgtcta gatttttagg gttaaagcaa
     7981 tcaatcacct gacgattcaa ggtggttgga tcatgacgat tccagaaaac atcaagcaag
     8041 ctctcaaagc tacactcttt gggatcatac tgaactctaa caacctcgtt atgtcccgta
     8101 gtgccagtac agacatcctc gtaactcgga ttgtgcacga tgccatggct atacccaacc
     8161 tcggtcttgg tcacaccagg aactctctgg taagctagct ccactcccca gaaacaaccg
     8221 gcgccaaatt gcgcgaattg ctgacctgaa gacggaacat catcgtcggg tccttgggcg
     8281 attgcggcgg aagatgggtc agcttgggct tgaggacgag acccgaatcc gagtctgttg
     8341 aaaaggttgt tcattgggga tttgtatacg gagattggtc gtcgagaggt ttgagggaaa
     8401 ggacaaatgg gtttggctct ggagaaagag agtgcggctt tagagagaga attgagaggt
     8461 ttagagagag atgcggcggc gatgagcgga ggagagacga cgaggacctg cattatcaaa
     8521 gcagtgacgt ggtgaaattt ggaactttta agaggcagat agatttatta tttgtatcca
     8581 ttttcttcat tgttctagaa tgtcgcggaa caaattttaa aactaaatcc taaatttttc
     8641 taattttgtt gccaatagtg gatatgtggg ccgtatagaa ggaatctatt gaaggcccaa
     8701 acccatactg acgagcccaa aggttcgttt tgcgttttat gtttcggttc gatgccaacg
     8761 ccacattctg agctaggcaa aaaacaaacg tgtctttgaa tagactcctc tcgttaacac
     8821 atgcagcggc tgcatggtga cgccattaac acgtggccta caattgcatg atgtctccat
     8881 tgacacgtga cttctcgtct cctttcttaa tatatctaac aaacactcct acctcttcca
     8941 aaatatatac acatcttttt gatcaatctc tcattcaaaa tctcattctc tctagtaaac
     9001 aagaacaaaa aaatggcgga tacagctaga ggaacccatc acgatatcat cggcagagac
     9061 cagtacccga tgatgggccg agaccgagac cagtaccaga tgtccggacg aggatctgac
     9121 tactccaagt ctaggcagat tgctaaagct gcaactgctg tcacagctgg tggttccctc
     9181 cttgttctct ccagccttac ccttgttgga actgtcatag ctttgactgt tgcaacacct
     9241 ctgctcgtta tcttcagccc aatccttgtc ccggctctca tcacagttgc actcctcatc
     9301 accggttttc tttcctctgg agggtttggc attgccgcta taaccgtttt ctcttggatt
     9361 tacaagtaag cacacattta tcatcttact tcataatttt gtgcaatatg tgcatgcatg
     9421 tgttgagcca gtagctttgg atcaattttt ttggtcgaat aacaaatgta acaataagaa
     9481 attgcaaatt ctagggaaca tttggttaac taaatacgaa atttgaccta gctagcttga
     9541 atgtgtctgt gtatatcatc tatataggta aaatgcttgg tatgatacct attgattgtg
     9601 aataggtacg caacgggaga gcacccacag ggatcagaca agttggacag tgcaaggatg
     9661 aagttgggaa gcaaagctca ggatctgaaa gacagagctc agtactacgg acagcaacat
     9721 actggtgggg aacatgaccg tgaccgtact cgtggtggcc agcacactac tatgagcgag
     9781 ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac
     9841 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc
     9901 aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg
     9961 tacggcagca gaaccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc
    10021 ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc
    10081 gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg
    10141 gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccaac
    10201 accgagatgc tgtaccccgc tgacggcggc ctggaaggca gaagcgacat ggccctgaag
    10261 ctcgtgggcg ggggccacct gatctgcaac ttcaagacca catacagatc caagaaaccc
    10321 gctaagaacc tcaagatgcc cggcgtctac tatgtggacc acagactgga aagaatcaag
    10381 gaggccgaca aagagaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac
    10441 ctccctagca aactggggca caagtgaatc gttcaaacat ttggcaataa agtttcttaa
    10501 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta
    10561 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
    10621 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
    10681 ataaattatc gcgcgcggtg tcatctatgt tactagatca ctagtgcggc cgcctgcagg
    10741 tcgaccatat gggagagctc aagcttagct tgagcttgga tcagattgtc gtttcccgcc
    10801 ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga
    10861 gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat
    10921 ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtacttta aagtacttta
    10981 aagtacttta aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac
    11041 gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg
    11101 ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga
    11161 atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc
    11221 tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc
    11281 acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc
    11341 cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc
    11401 tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg
    11461 ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc
    11521 gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc
    11581 gcacccggag cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc ccccgcccta
    11641 ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg
    11701 tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc
    11761 gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat
    11821 tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa
    11881 accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat
    11941 gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga
    12001 aatcctggcc ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga
    12061 agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca
    12121 tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg
    12181 aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat
    12241 ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag
    12301 ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc
    12361 gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc
    12421 gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg
    12481 ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg
    12541 gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg
    12601 gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg
    12661 cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc
    12721 acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc
    12781 gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca
    12841 caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca
    12901 gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca
    12961 ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat
    13021 acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg
    13081 gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc
    13141 atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgtctg ccggccctgc
    13201 aatggcactg gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc
    13261 cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg
    13321 ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg
    13381 ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga
    13441 agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca
    13501 cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac
    13561 gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc
    13621 cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa
    13681 ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc
    13741 cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg
    13801 acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga
    13861 aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca
    13921 agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga
    13981 tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact
    14041 ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca
    14101 aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt
    14161 tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg
    14221 atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga
    14281 tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc
    14341 tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac attgggaacc
    14401 caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga
    14461 accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa
    14521 actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc
    14581 agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc
    14641 ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc
    14701 caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac
    14761 atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
    14821 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
    14881 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
    14941 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
    15001 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
    15061 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
    15121 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
    15181 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    15241 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    15301 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    15361 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    15421 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    15481 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    15541 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    15601 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    15661 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
    15721 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    15781 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    15841 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    15901 tgcatgatat atctcccaat ttgtgtaggg cttattatgc acgcttaaaa ataataaaag
    15961 cagacttgac ctgatagttt ggctgtgagc aattatgtgc ttagtgcatc taacgcttga
    16021 gttaagccgc gccgcgaagc ggcgtcggct tgaacgaatt tctagctaga cattatttgc
    16081 cgactacctt ggtgatctcg cctttcacgt agtggacaaa ttcttccaac tgatctgcgc
    16141 gcgaggccaa gcgatcttct tcttgtccaa gataagcctg tctagcttca agtatgacgg
    16201 gctgatactg ggccggcagg cgctccattg cccagtcggc agcgacatcc ttcggcgcga
    16261 ttttgccggt tactgcgctg taccaaatgc gggacaacgt aagcactaca tttcgctcat
    16321 cgccagccca gtcgggcggc gagttccata gcgttaaggt ttcatttagc gcctcaaata
    16381 gatcctgttc aggaaccgga tcaaagagtt cctccgccgc tggacctacc aaggcaacgc
    16441 tatgttctct tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg gctggctcga
    16501 agatacctgc aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg
    16561 gataacgcca cggaatgatg tcgtcgtgca caacaatggt gacttctaca gcgcggagaa
    16621 tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg
    16681 ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca
    16741 ggccgccatc cactgcggag ccgtacaaat gtacggccag caacgtcggt tcgagatggc
    16801 gctcgatgac gccaactacc tctgatagtt gagtcgatac ttcggcgatc accgcttccc
    16861 ccatgatgtt taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc
    16921 ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag
    16981 actgtacccc aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac
    17041 cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca
    17101 gcttacgaac cgaacaggct tatgtccact gggttcgtgc ccgaattgat cacaggcagc
    17161 aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa
    17221 cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc
    17281 cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt
    17341 gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt
    17401 ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac
    17461 tgaattaacg ccgaattgaa ttatcagctt gcatgccggt cgatctagta acatagatga
    17521 caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta
    17581 aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta
    17641 catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag
    17701 accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatctgcttg
    17761 actctaggga attaattcct gaatcactgc gaccggccct cccgcgaccc agccgagcga
    17821 gcttagcgaa ctgtggacga gaactgtgcc accaagcgta aggccgttct ctcgcatttg
    17881 ccttgctagg ctcgcgcgag ttgctggctg aggcgttctc gaaatcagct cttgttcggt
    17941 cggcatctac tctattcctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg
    18001 gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta
    18061 cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct
    18121 gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc
    18181 atatacgccc ggagccttgg cgatcctgca agctccggat gcctccgctc gaagtagcgc
    18241 gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat
    18301 tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc
    18361 gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc
    18421 tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc
    18481 atcacagttt gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat
    18541 gtagtgtatt gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga
    18601 tcggccgcag cgatagcatc catagcctcc gcgaccggct gaagaacagc gggcagttcg
    18661 gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg
    18721 ctctcgctga actccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag
    18781 tgccgataaa cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca
    18841 tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc
    18901 atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg
    18961 agttcaggct ttttcatagg ggggattcga gttgagagtg aatatgagac tctaattgga
    19021 taccgagggg aatttatgga acgtcagtgg agcatttttg acaagaaata tttgctagct
    19081 gatagtgacc ttaggcgact tttgaacgcg caataatggt ttctgacgta tgtgcttagc
    19141 tcattaaact ccagaaaccc gcggctcagt ggctccttca acgttgcggt tctgtcagtt
    19201 ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt cataacgtga ctcccttaat
    19261 tctccgctca tgatcgataa ttcgcggtac ccggggatcc tctagagggc ccgacgtcgc
    19321 atgctcccgg ccgccatggc cgcgggatat cacaagtttg tacaaaaaag caggctccgc
    19381 ggccgctcgt tgaacaacgg aaactcgact tgccttccgc acaatacatc atttcttctt
    19441 agcttttttt cttcttcttc gttcatacag tttttttttg tttatcagct tacattttct
    19501 tgaaccgtag ctttcgtttt cttcttttta actttccatt cggagttttt gtatcttgtt
    19561 tcatagtttg tcccaggatt agaatgatta ggcatcgaac cttcaagaat ttgatt
//
