LOCUS       sequence-221763-       19607 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  binary vector for INF (Induced Neo-Functionalization) of FT in 
            Arabidopsis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19607)
  AUTHORS   Kang BC, Yun JY, Kim ST, Shin Y, Ryu J, Choi M, Woo JW, Kim JS
  TITLE     Precision genome engineering through adenine base editing in plants.
  JOURNAL   Nat Plants. 2018 Jun 4. pii: 10.1038/s41477-018-0178-x. doi: 
            10.1038/s41477-018-0178-x.
  PUBMED    29867128
REFERENCE   2  (bases 1 to 19607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2018 Jun 4. pii: 10.1038/s41477-018-0178-x. doi: 
            10.1038/s41477-018-0178-x."
COMMENT     Sequence Label: pJY-RpABE-FT_INF
FEATURES             Location/Qualifiers
     source          1..19607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        268..343
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             join(3223..3246,3247..3249,3250..7323)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7336..7356
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7370..7618
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     protein_bind    complement(7627..7651)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9797..10492
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     terminator      10493..10744
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    10848..10872
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12203..12832
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             13261..14334
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      14400..14594
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(14830..14852)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    14938..15078
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     14993..15012
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(15093..15110)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15264..15852)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15344..15363)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(16098..16889)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    17410..17434
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(17527..17779)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(17977..19002)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(19038..19217)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     regulatory      19355..19364
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    19377..19401
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aatgattagg catcgaacct tcaagaattt gattgaataa aacatcttca ttcttaagat
       61 atgaagataa tcttcaaaag gcccctggga atctgaaaga agagaagcag gcccatttat
      121 atgggaaaga acaatagtat ttcttatata ggcccattta agttgaaaac aatcttcaaa
      181 agtcccacat cgcttagata agaaaacgaa gctgagttta tatacagcta gagtcgaagt
      241 agtgattgga gatattctcg gaggtgagtt ttagagctag aaatagcaag ttaaaataag
      301 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt ttttgcggcc
      361 gcgatccctc aacttttgat tcgctatttg cagtgcacct gtggcgttca tcacatcttt
      421 tgtgacactg tttgcactgg tcattgctat tacaaaggac cttcctgatg ttgaaggaga
      481 tcgaaagtaa gtaactgcac gcataaccat tttctttccg ctctttggct caatccattt
      541 gacagtcaaa gacaatgttt aaccagctcc gtttgatata ttgtctttat gtgtttgttc
      601 aagcatgttt agttaatcat gcctttgatt gatcttgaat aggttccaaa tatcaaccct
      661 ggcaacaaaa cttggagtga gaaacattgc attcctcggt tctggacttc tgctagtaaa
      721 ttatgtttca gccatatcac tagctttcta catgcctcag gtgaattcat ctatttccgt
      781 cttaactatt tcggttaatt aaagcacgaa caccattact gcatgtagaa gcttgataaa
      841 ctatcgccac caatttattt ttgttgcgat attgttactt tcctcagtat gcagctttga
      901 aaagaccaac cctcttatcc tttaacaatg aacaggtttt tagaggtagc ttgatgattc
      961 ctgcacatgt gatcttggct tcaggcttaa ttttccaggt aaagcattat gagatactct
     1021 tatatctctt acatactttt gagataatgc acaagaactt cataactata tgctttagtt
     1081 tctgcatttg acactgccaa attcattaat ctctaatatc tttgttgttg atctttggta
     1141 gacatgggta ctagaaaaag caaactacac caaggtaaaa tacttttgta caaacataaa
     1201 ctcgttatca cggaacatca atggagtgta tatctaacgg agtgtagaaa catttgatta
     1261 ttgcaggaag ctatctcagg atattatcgg tttatatgga atctcttcta cgcagagtat
     1321 ctgttattcc ccttcctcta gctttcaatt tcatggtgag gatatgcagt tttctttgta
     1381 tatcattctt cttcttcttt gtagcttgga gtcaaaatcg gttccttcat gtacatacat
     1441 caaggatatg tccttctgaa tttttatatc ttgcaataaa aatgcttgta ccaattgaaa
     1501 caccagcttt ttgagttcta tgatcactga cttggttcta accaaaaaaa aaaaatgttt
     1561 aatttacata tctaaaagta ggtttaggga aacctaaaca gtaaaatatt tgtatattat
     1621 tcgaatttca ctcatcataa aaacttaaat tgcaccataa aattttgttt tactattaat
     1681 gatgtaattt gtgtaactta agataaaaat aatattccgt aagttaaccg gctaaaacca
     1741 cgtataaacc agggaacctg ttaaaccggt tctttactgg ataaagaaat gaaagcccat
     1801 gtagacagct ccattagagc ccaaacccta aatttctcat ctatataaaa ggagtgacat
     1861 tagggttttt gttcgtcctc ttaaagcttc tcgttttctc tgccgtctct ctcattcgcg
     1921 cgacgcaaac gatcttcagg tgatcttctt tctccaaatc ctctctcata actctgattt
     1981 cgtacttgtg tatttgagct cacgctctgt ttctctcacc acagccccat gatgagcgag
     2041 gtcgagttta gccatgaata ctggatgaga catgctctta ctttagcgaa acgtgcgtgg
     2101 gacgagagag aggttccggt gggcgcggtg ttagtacaca ataacagggt tatcggcgaa
     2161 gggtggaaca ggcctattgg caggcacgat ccgactgcac atgcggagat catggcgcta
     2221 cgacaggggg ggctcgtcat gcagaattat cgattgatag acgccacgct gtacgtcact
     2281 ctggaaccat gcgtaatgtg tgcaggagcg atgatccact ctagaattgg aagagtagtc
     2341 ttcggcgcga gggatgcaaa gaccggggcg gcggggtccc taatggacgt gcttcaccac
     2401 cctggcatga accatagagt ggagatcacc gagggcatac ttgctgatga atgtgcagca
     2461 cttctatccg atttctttcg aatgagaagg caggaaatta aagcccagaa gaaggctcaa
     2521 tctagtacag actctggagg gtccagtggc ggctcatccg ggagcgaaac accagggacg
     2581 tcagaatccg ctacgcccga atctagtggt ggctccagcg gaggaagtag tgaggtggag
     2641 ttctctcacg aatactggat gcgacatgct ctaacgctag caaaacgagc gagggatgaa
     2701 cgagaggttc ctgtaggagc agtgttggtt ctgaacaaca gagttattgg tgaaggttgg
     2761 aatcgtgcta ttgggcttca cgacccaaca gcccatgccg aaataatggc gctcaggcaa
     2821 ggaggcttag taatgcaaaa ctacagatta atcgacgcga ccctgtatgt cacgttcgag
     2881 ccatgcgtta tgtgcgcggg cgcgatgatt cattctagaa ttggaagggt tgtttttggg
     2941 gtgagaaatg ccaaaactgg tgcggctggg agtcttatgg acgttctcca ctaccctggt
     3001 atgaaccatc gagtggaaat cacggagggg attttggcgg acgaatgtgc agcattgtta
     3061 tgctatttct tccgtatgcc taggcaggtc ttcaacgctc agaagaaggc gcaaagttca
     3121 accgattcag gggggtcttc tggcggatct tctggttcag agacgcccgg tacctcagaa
     3181 tcagctacgc ctgagagttc aggtgggtct tctggcggca gcgacaagaa gtacagcatc
     3241 ggcctggcca tcggtaccaa cagcgtgggc tgggccgtga tcaccgacga gtacaaggtg
     3301 cccagcaaga agttcaaggt gctgggcaac accgaccgcc acagcatcaa gaagaacctg
     3361 atcggcgccc tgctgttcga cagcggcgag accgccgagg ccacccgcct gaagcgcacc
     3421 gcccgccgcc gctacacccg ccgcaagaac cgcatctgct acctgcagga gatcttcagc
     3481 aacgagatgg ccaaggtgga cgacagcttc ttccaccgcc tggaggagag cttcctggtg
     3541 gaggaggaca agaagcacga gcgccacccc atcttcggca acatcgtgga cgaggtggcc
     3601 taccacgaga agtaccccac catctaccat ctgcgcaaga agctggtgga cagcaccgac
     3661 aaggccgacc tgcgcctgat ctacctggcc ctggcccaca tgatcaagtt ccgcggccac
     3721 ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct gttcatccag
     3781 ctggtgcaga cctacaacca gctgttcgag gagaacccca tcaacgccag cggcgtggac
     3841 gccaaggcca tcctgagcgc ccgcctgagc aagagccgcc gcctggagaa cctgatcgcc
     3901 cagctgcccg gcgagaagaa gaacggcctg ttcggcaacc tgatcgccct gagcctgggc
     3961 ctgaccccca acttcaagag caacttcgac ctggccgagg acgccaagct gcagctgagc
     4021 aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga ccagtacgcc
     4081 gacctgttcc tggccgccaa gaacctgagc gacgccatcc tgctgagcga catcctgcgc
     4141 gtgaacaccg agatcaccaa ggcccccctg agcgccagca tgatcaagcg ctacgacgag
     4201 caccaccagg acctgaccct gctgaaggcc ctggtgcgcc agcagctgcc cgagaagtac
     4261 aaggagatct tcttcgacca gagcaagaac ggctacgccg gctacatcga cggcggcgcc
     4321 agccaggagg agttctacaa gttcatcaag cccatcctgg agaagatgga cggcaccgag
     4381 gagctgctgg tgaagctgaa ccgcgaggac ctgctgcgca agcagcgcac cttcgacaac
     4441 ggcagcatcc cccaccagat ccacctgggc gagctgcacg ccatcctgcg ccgccaggag
     4501 gacttctacc ccttcctgaa ggacaaccgc gagaagatcg agaagatcct gaccttccgc
     4561 atcccctact acgtgggccc cctggcccgc ggcaacagcc gcttcgcctg gatgacccgc
     4621 aagagcgagg agaccatcac cccctggaac ttcgaggagg tggtggacaa gggcgccagc
     4681 gcccagagct tcatcgagcg catgaccaac ttcgacaaga acctgcccaa cgagaaggtg
     4741 ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt acaacgagct gaccaaggtg
     4801 aagtacgtga ccgagggcat gcgcaagccc gccttcctga gcggcgagca gaagaaggcc
     4861 atcgtggacc tgctgttcaa gaccaaccgc aaggtgaccg tgaagcagct gaaggaggac
     4921 tacttcaaga agatcgagtg cttcgacagc gtggagatca gcggcgtgga ggaccgcttc
     4981 aacgccagcc tgggcaccta ccacgacctg ctgaagatca tcaaggacaa ggacttcctg
     5041 gacaacgagg agaacgagga catcctggag gacatcgtgc tgaccctgac cctgttcgag
     5101 gaccgcgaga tgatcgagga gcgcctgaag acctacgccc acctgttcga cgacaaggtg
     5161 atgaagcagc tgaagcgccg ccgctacacc ggctggggcc gcctgagccg caagcttatc
     5221 aacggcatcc gcgacaagca gagcggcaag accatcctgg acttcctgaa gagcgacggc
     5281 ttcgccaacc gcaacttcat gcagctgatc cacgacgaca gcctgacctt caaggaggac
     5341 atccagaagg cccaggtgag cggccagggc gacagcctgc acgagcacat cgccaacctg
     5401 gccggcagcc ccgccatcaa gaagggcatc ctgcagaccg tgaaggtggt ggacgagctg
     5461 gtgaaggtga tgggccgcca caagcccgag aacatcgtga tcgagatggc ccgcgagaac
     5521 cagaccaccc agaagggcca gaagaacagc cgcgagcgca tgaagcgcat cgaggagggc
     5581 atcaaggagc tgggcagcca gatcctgaag gagcaccccg tggagaacac ccagctgcag
     5641 aacgagaagc tgtacctgta ctacctgcag aacggccgcg acatgtacgt ggaccaggag
     5701 ctggacatca accgcctgag cgactacgac gtggaccaca tcgtgcccca gagcttcctg
     5761 aaggacgaca gcatcgacaa caaggtgctg acccgcagcg acaagaaccg cggcaagagc
     5821 gacaacgtgc ccagcgagga ggtggtgaag aagatgaaga actactggcg ccagctgctg
     5881 aacgccaagc tgatcaccca gcgcaagttc gacaacctga ccaaggccga gcgcggcggc
     5941 ctgagcgagc tggacaaggc cggcttcatc aagcgccagc tggtggagac ccgccagatc
     6001 accaagcacg tggcccagat cctggacagc cgcatgaaca ccaagtacga cgagaacgac
     6061 aagctgatcc gcgaggtgaa ggtgatcacc ctgaagagca agctggtgag cgacttccgc
     6121 aaggacttcc agttctacaa ggtgcgcgag atcaacaact accaccacgc ccacgacgcc
     6181 tacctgaacg ccgtggtggg caccgccctg atcaagaagt accccaagct ggagagcgag
     6241 ttcgtgtacg gcgactacaa ggtgtacgac gtgcgcaaga tgatcgccaa gagcgagcag
     6301 gagatcggca aggccaccgc caagtacttc ttctacagca acatcatgaa cttcttcaag
     6361 accgagatca ccctggccaa cggcgagatc cgcaagcgcc ccctgatcga gaccaacggc
     6421 gagaccggcg agatcgtgtg ggacaagggc cgcgacttcg ccaccgtgcg caaggtgctg
     6481 agcatgcccc aggtgaacat cgtgaagaag accgaggtgc agaccggcgg cttcagcaag
     6541 gagagcatcc tgcccaagcg caacagcgac aagctgatcg cccgcaagaa ggactgggac
     6601 cccaagaagt acggcggctt cgacagcccc accgtggcct acagcgtgct ggtggtggcc
     6661 aaggtggaga agggcaagag caagaagctg aagagcgtga aggagctgct gggcatcacc
     6721 atcatggagc gcagcagctt cgagaagaac cccatcgact tcctggaggc caagggctac
     6781 aaggaggtga agaaggacct gatcatcaag ctgcccaagt acagcctgtt cgagctggag
     6841 aacggccgca agcgcatgct ggccagcgcc ggcgagctgc agaagggcaa cgagctggcc
     6901 ctgcccagca agtacgtgaa cttcctgtac ctggccagcc actacgagaa gctgaagggc
     6961 agccccgagg acaacgagca gaagcagctg ttcgtggagc agcacaagca ctacctggac
     7021 gagatcatcg agcagatcag cgagttcagc aagcgcgtga tcctggccga cgccaacctg
     7081 gacaaggtgc tgagcgccta caacaagcac cgcgacaagc ccatccgcga gcaggccgag
     7141 aacatcatcc acctgttcac cctgaccaac ctgggcgccc ccgccgcctt caagtacttc
     7201 gacaccacca tcgaccgcaa gcgctacacc agcaccaagg aggtgctgga cgccaccctg
     7261 atccaccaga gcatcaccgg tctgtacgag acccgcatcg acctgagcca gctgggcggc
     7321 gactctggtg gttctccaaa gaaaaagaga aaagtataaa agggtgggat atgaagatga
     7381 agatgaaata tttggtgtgt caaataaaaa gcttgtgtgc ttaagtttgt gtttttttct
     7441 tggcttgttg tgttatgaat ttgtggcttt ttctaatatt aaatgaatgt aagatctcat
     7501 tataatgaat aaacaaatgt ttctataatc cattgtgaat gttttgttgg atctcttctg
     7561 cagcatataa ctactgtatg tgctatggta tggactatgg aatatgatta aagataaggc
     7621 gcgccgaccc agctttcttg tacaaagtgg tgatatcact agtgtatgta ggtatagtaa
     7681 catgaacaag aacagactca agtacaagat cgcatacgaa aatggaaatg gcaatgtcac
     7741 ttccacataa tcaaacacga atcctcatat caacaaggcc tgagattcta actagctcat
     7801 aacaacttag ccaatagtta cttgagacta ccaaatgtat gtagaactaa agactaaggg
     7861 acagagagtt cgtctaaaca ggtgaatcta gtcgttgtta tctaataaac aattcagccc
     7921 caaatgcaga acacacatag agctctctat tgattcaaat tacgatctga tactgataac
     7981 gtctagattt ttagggttaa agcaatcaat cacctgacga ttcaaggtgg ttggatcatg
     8041 acgattccag aaaacatcaa gcaagctctc aaagctacac tctttgggat catactgaac
     8101 tctaacaacc tcgttatgtc ccgtagtgcc agtacagaca tcctcgtaac tcggattgtg
     8161 cacgatgcca tggctatacc caacctcggt cttggtcaca ccaggaactc tctggtaagc
     8221 tagctccact ccccagaaac aaccggcgcc aaattgcgcg aattgctgac ctgaagacgg
     8281 aacatcatcg tcgggtcctt gggcgattgc ggcggaagat gggtcagctt gggcttgagg
     8341 acgagacccg aatccgagtc tgttgaaaag gttgttcatt ggggatttgt atacggagat
     8401 tggtcgtcga gaggtttgag ggaaaggaca aatgggtttg gctctggaga aagagagtgc
     8461 ggctttagag agagaattga gaggtttaga gagagatgcg gcggcgatga gcggaggaga
     8521 gacgacgagg acctgcatta tcaaagcagt gacgtggtga aatttggaac ttttaagagg
     8581 cagatagatt tattatttgt atccattttc ttcattgttc tagaatgtcg cggaacaaat
     8641 tttaaaacta aatcctaaat ttttctaatt ttgttgccaa tagtggatat gtgggccgta
     8701 tagaaggaat ctattgaagg cccaaaccca tactgacgag cccaaaggtt cgttttgcgt
     8761 tttatgtttc ggttcgatgc caacgccaca ttctgagcta ggcaaaaaac aaacgtgtct
     8821 ttgaatagac tcctctcgtt aacacatgca gcggctgcat ggtgacgcca ttaacacgtg
     8881 gcctacaatt gcatgatgtc tccattgaca cgtgacttct cgtctccttt cttaatatat
     8941 ctaacaaaca ctcctacctc ttccaaaata tatacacatc tttttgatca atctctcatt
     9001 caaaatctca ttctctctag taaacaagaa caaaaaaatg gcggatacag ctagaggaac
     9061 ccatcacgat atcatcggca gagaccagta cccgatgatg ggccgagacc gagaccagta
     9121 ccagatgtcc ggacgaggat ctgactactc caagtctagg cagattgcta aagctgcaac
     9181 tgctgtcaca gctggtggtt ccctccttgt tctctccagc cttacccttg ttggaactgt
     9241 catagctttg actgttgcaa cacctctgct cgttatcttc agcccaatcc ttgtcccggc
     9301 tctcatcaca gttgcactcc tcatcaccgg ttttctttcc tctggagggt ttggcattgc
     9361 cgctataacc gttttctctt ggatttacaa gtaagcacac atttatcatc ttacttcata
     9421 attttgtgca atatgtgcat gcatgtgttg agccagtagc tttggatcaa tttttttggt
     9481 cgaataacaa atgtaacaat aagaaattgc aaattctagg gaacatttgg ttaactaaat
     9541 acgaaatttg acctagctag cttgaatgtg tctgtgtata tcatctatat aggtaaaatg
     9601 cttggtatga tacctattga ttgtgaatag gtacgcaacg ggagagcacc cacagggatc
     9661 agacaagttg gacagtgcaa ggatgaagtt gggaagcaaa gctcaggatc tgaaagacag
     9721 agctcagtac tacggacagc aacatactgg tggggaacat gaccgtgacc gtactcgtgg
     9781 tggccagcac actactatga gcgagctgat taaggagaac atgcacatga agctgtacat
     9841 ggagggcacc gtgaacaacc accacttcaa gtgcacatcc gagggcgaag gcaagcccta
     9901 cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt
     9961 cgacatcctg gctaccagct tcatgtacgg cagcagaacc ttcatcaacc acacccaggg
    10021 catccccgac ttctttaagc agtccttccc tgagggcttc acatgggaga gagtcaccac
    10081 atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct
    10141 catctacaac gtcaagatca gaggggtgaa cttcccatcc aacggccctg tgatgcagaa
    10201 gaaaacactc ggctgggagg ccaacaccga gatgctgtac cccgctgacg gcggcctgga
    10261 aggcagaagc gacatggccc tgaagctcgt gggcgggggc cacctgatct gcaacttcaa
    10321 gaccacatac agatccaaga aacccgctaa gaacctcaag atgcccggcg tctactatgt
    10381 ggaccacaga ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg agcagcacga
    10441 ggtggctgtg gccagatact gcgacctccc tagcaaactg gggcacaagt gaatcgttca
    10501 aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc
    10561 atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta
    10621 tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa
    10681 aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta
    10741 gatcactagt gcggccgcct gcaggtcgac catatgggag agctcaagct tagcttgagc
    10801 ttggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat
    10861 tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg
    10921 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg
    10981 gatcaaagta ctttaaagta ctttaaagta ctttaaagta ctttgatcca acccctccgc
    11041 tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg
    11101 cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc
    11161 gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg
    11221 aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac
    11281 ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag
    11341 atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct
    11401 ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg
    11461 gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg
    11521 gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag
    11581 ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga
    11641 ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag
    11701 ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc
    11761 tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg
    11821 cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat
    11881 gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca
    11941 ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc
    12001 acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg
    12061 cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg
    12121 tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata
    12181 aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc
    12241 aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt
    12301 tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga
    12361 tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat
    12421 cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc
    12481 cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg
    12541 ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct
    12601 acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc
    12661 cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag
    12721 ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc
    12781 tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga
    12841 ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc
    12901 agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac
    12961 tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag
    13021 accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga
    13081 ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc
    13141 aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag
    13201 cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc
    13261 gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg
    13321 gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc
    13381 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg
    13441 gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc
    13501 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt
    13561 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac
    13621 gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg
    13681 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga
    13741 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga
    13801 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg
    13861 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag
    13921 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac
    13981 atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac
    14041 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac
    14101 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac
    14161 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc
    14221 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc
    14281 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg
    14341 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct
    14401 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt
    14461 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag
    14521 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc
    14581 ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc
    14641 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc
    14701 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg
    14761 tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga
    14821 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
    14881 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
    14941 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
    15001 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
    15061 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    15121 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    15181 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    15241 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    15301 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    15361 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    15421 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    15481 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    15541 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    15601 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    15661 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
    15721 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    15781 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    15841 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    15901 aactcacgtt aagggatttt ggtcatgcat gatatatctc ccaatttgtg tagggcttat
    15961 tatgcacgct taaaaataat aaaagcagac ttgacctgat agtttggctg tgagcaatta
    16021 tgtgcttagt gcatctaacg cttgagttaa gccgcgccgc gaagcggcgt cggcttgaac
    16081 gaatttctag ctagacatta tttgccgact accttggtga tctcgccttt cacgtagtgg
    16141 acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa
    16201 gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag
    16261 tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac
    16321 aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt
    16381 aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc
    16441 gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga
    16501 tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct
    16561 ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca
    16621 atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa
    16681 aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc
    16741 aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg
    16801 gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc
    16861 gatacttcgg cgatcaccgc ttcccccatg atgtttaact ttgttttagg gcgactgccc
    16921 tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct
    16981 tgctgcttgg atgcccgagg catagactgt accccaaaaa aacagtcata acaagccatg
    17041 aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt
    17101 gagcgcatac gctacttgca ttacagctta cgaaccgaac aggcttatgt ccactgggtt
    17161 cgtgcccgaa ttgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
    17221 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
    17281 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
    17341 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
    17401 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
    17461 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttgaattatc agcttgcatg
    17521 ccggtcgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct
    17581 atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc
    17641 atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca
    17701 gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt
    17761 gccaaatgtt tgaacgatct gcttgactct agggaattaa ttcctgaatc actgcgaccg
    17821 gccctcccgc gacccagccg agcgagctta gcgaactgtg gacgagaact gtgccaccaa
    17881 gcgtaaggcc gttctctcgc atttgccttg ctaggctcgc gcgagttgct ggctgaggcg
    17941 ttctcgaaat cagctcttgt tcggtcggca tctactctat tcctttgccc tcggacgagt
    18001 gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc
    18061 cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc
    18121 gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata
    18181 gagttggtca agaccaatgc ggagcatata cgcccggagc cttggcgatc ctgcaagctc
    18241 cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca
    18301 gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat
    18361 gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac
    18421 gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc
    18481 gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc
    18541 aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg
    18601 gccgaacccg ctcgtctggc taagatcggc cgcagcgata gcatccatag cctccgcgac
    18661 cggctgaaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtgc
    18721 acggcgggag atgcaatagg tcaggctctc gctgaactcc ccaatgtcaa gcacttccgg
    18781 aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc
    18841 ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg
    18901 agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact tttcgatcag
    18961 aaacttctcg acagacgtcg cggtgagttc aggctttttc atagggggga ttcgagttga
    19021 gagtgaatat gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat
    19081 ttttgacaag aaatatttgc tagctgatag tgaccttagg cgacttttga acgcgcaata
    19141 atggtttctg acgtatgtgc ttagctcatt aaactccaga aacccgcggc tcagtggctc
    19201 cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg
    19261 ggggtcataa cgtgactccc ttaattctcc gctcatgatc gataattcgc ggtacccggg
    19321 gatcctctag agggcccgac gtcgcatgct cccggccgcc atggccgcgg gatatcacaa
    19381 gtttgtacaa aaaagcaggc tccgcggccg ctcgttgaac aacggaaact cgacttgcct
    19441 tccgcacaat acatcatttc ttcttagctt tttttcttct tcttcgttca tacagttttt
    19501 ttttgtttat cagcttacat tttcttgaac cgtagctttc gttttcttct ttttaacttt
    19561 ccattcggag tttttgtatc ttgtttcata gtttgtccca ggattag
//
