LOCUS       sequence-221764-       19608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  binary vector for IMS (Induced Mis-Splicing) of PDS3 in Arabidopsis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19608)
  AUTHORS   Kang BC, Yun JY, Kim ST, Shin Y, Ryu J, Choi M, Woo JW, Kim JS
  TITLE     Precision genome engineering through adenine base editing in plants.
  JOURNAL   Nat Plants. 2018 Jun 4. pii: 10.1038/s41477-018-0178-x. doi: 
            10.1038/s41477-018-0178-x.
  PUBMED    29867128
REFERENCE   2  (bases 1 to 19608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2018 Jun 4. pii: 10.1038/s41477-018-0178-x. doi: 
            10.1038/s41477-018-0178-x."
COMMENT     Sequence Label: pJY-RpABE-PDS3_IMS
FEATURES             Location/Qualifiers
     source          1..19608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        319..394
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             join(3274..3297,3298..3300,3301..7374)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7387..7407
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7421..7669
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     protein_bind    complement(7678..7702)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9848..10543
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     terminator      10544..10795
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    10899..10923
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12254..12883
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             13312..14385
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      14451..14645
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(14881..14903)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    14989..15129
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     15044..15063
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(15144..15161)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15315..15903)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15395..15414)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(16149..16940)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    17461..17485
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(17578..17830)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(18028..19053)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(19089..19268)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     regulatory      19406..19415
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    19428..19452
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttccattcg gagtttttgt atcttgtttc atagtttgtc ccaggattag aatgattagg
       61 catcgaacct tcaagaattt gattgaataa aacatcttca ttcttaagat atgaagataa
      121 tcttcaaaag gcccctggga atctgaaaga agagaagcag gcccatttat atgggaaaga
      181 acaatagtat ttcttatata ggcccattta agttgaaaac aatcttcaaa agtcccacat
      241 cgcttagata agaaaacgaa gctgagttta tatacagcta gagtcgaagt agtgattgcc
      301 tccagatagc tgcatggagt tttagagcta gaaatagcaa gttaaaataa ggctagtccg
      361 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tttttgcggc cgcgatccct
      421 caacttttga ttcgctattt gcagtgcacc tgtggcgttc atcacatctt ttgtgacact
      481 gtttgcactg gtcattgcta ttacaaagga ccttcctgat gttgaaggag atcgaaagta
      541 agtaactgca cgcataacca ttttctttcc gctctttggc tcaatccatt tgacagtcaa
      601 agacaatgtt taaccagctc cgtttgatat attgtcttta tgtgtttgtt caagcatgtt
      661 tagttaatca tgcctttgat tgatcttgaa taggttccaa atatcaaccc tggcaacaaa
      721 acttggagtg agaaacattg cattcctcgg ttctggactt ctgctagtaa attatgtttc
      781 agccatatca ctagctttct acatgcctca ggtgaattca tctatttccg tcttaactat
      841 ttcggttaat taaagcacga acaccattac tgcatgtaga agcttgataa actatcgcca
      901 ccaatttatt tttgttgcga tattgttact ttcctcagta tgcagctttg aaaagaccaa
      961 ccctcttatc ctttaacaat gaacaggttt ttagaggtag cttgatgatt cctgcacatg
     1021 tgatcttggc ttcaggctta attttccagg taaagcatta tgagatactc ttatatctct
     1081 tacatacttt tgagataatg cacaagaact tcataactat atgctttagt ttctgcattt
     1141 gacactgcca aattcattaa tctctaatat ctttgttgtt gatctttggt agacatgggt
     1201 actagaaaaa gcaaactaca ccaaggtaaa atacttttgt acaaacataa actcgttatc
     1261 acggaacatc aatggagtgt atatctaacg gagtgtagaa acatttgatt attgcaggaa
     1321 gctatctcag gatattatcg gtttatatgg aatctcttct acgcagagta tctgttattc
     1381 cccttcctct agctttcaat ttcatggtga ggatatgcag ttttctttgt atatcattct
     1441 tcttcttctt tgtagcttgg agtcaaaatc ggttccttca tgtacataca tcaaggatat
     1501 gtccttctga atttttatat cttgcaataa aaatgcttgt accaattgaa acaccagctt
     1561 tttgagttct atgatcactg acttggttct aaccaaaaaa aaaaaatgtt taatttacat
     1621 atctaaaagt aggtttaggg aaacctaaac agtaaaatat ttgtatatta ttcgaatttc
     1681 actcatcata aaaacttaaa ttgcaccata aaattttgtt ttactattaa tgatgtaatt
     1741 tgtgtaactt aagataaaaa taatattccg taagttaacc ggctaaaacc acgtataaac
     1801 cagggaacct gttaaaccgg ttctttactg gataaagaaa tgaaagccca tgtagacagc
     1861 tccattagag cccaaaccct aaatttctca tctatataaa aggagtgaca ttagggtttt
     1921 tgttcgtcct cttaaagctt ctcgttttct ctgccgtctc tctcattcgc gcgacgcaaa
     1981 cgatcttcag gtgatcttct ttctccaaat cctctctcat aactctgatt tcgtacttgt
     2041 gtatttgagc tcacgctctg tttctctcac cacagcccca tgatgagcga ggtcgagttt
     2101 agccatgaat actggatgag acatgctctt actttagcga aacgtgcgtg ggacgagaga
     2161 gaggttccgg tgggcgcggt gttagtacac aataacaggg ttatcggcga agggtggaac
     2221 aggcctattg gcaggcacga tccgactgca catgcggaga tcatggcgct acgacagggg
     2281 gggctcgtca tgcagaatta tcgattgata gacgccacgc tgtacgtcac tctggaacca
     2341 tgcgtaatgt gtgcaggagc gatgatccac tctagaattg gaagagtagt cttcggcgcg
     2401 agggatgcaa agaccggggc ggcggggtcc ctaatggacg tgcttcacca ccctggcatg
     2461 aaccatagag tggagatcac cgagggcata cttgctgatg aatgtgcagc acttctatcc
     2521 gatttctttc gaatgagaag gcaggaaatt aaagcccaga agaaggctca atctagtaca
     2581 gactctggag ggtccagtgg cggctcatcc gggagcgaaa caccagggac gtcagaatcc
     2641 gctacgcccg aatctagtgg tggctccagc ggaggaagta gtgaggtgga gttctctcac
     2701 gaatactgga tgcgacatgc tctaacgcta gcaaaacgag cgagggatga acgagaggtt
     2761 cctgtaggag cagtgttggt tctgaacaac agagttattg gtgaaggttg gaatcgtgct
     2821 attgggcttc acgacccaac agcccatgcc gaaataatgg cgctcaggca aggaggctta
     2881 gtaatgcaaa actacagatt aatcgacgcg accctgtatg tcacgttcga gccatgcgtt
     2941 atgtgcgcgg gcgcgatgat tcattctaga attggaaggg ttgtttttgg ggtgagaaat
     3001 gccaaaactg gtgcggctgg gagtcttatg gacgttctcc actaccctgg tatgaaccat
     3061 cgagtggaaa tcacggaggg gattttggcg gacgaatgtg cagcattgtt atgctatttc
     3121 ttccgtatgc ctaggcaggt cttcaacgct cagaagaagg cgcaaagttc aaccgattca
     3181 ggggggtctt ctggcggatc ttctggttca gagacgcccg gtacctcaga atcagctacg
     3241 cctgagagtt caggtgggtc ttctggcggc agcgacaaga agtacagcat cggcctggcc
     3301 atcggtacca acagcgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag
     3361 aagttcaagg tgctgggcaa caccgaccgc cacagcatca agaagaacct gatcggcgcc
     3421 ctgctgttcg acagcggcga gaccgccgag gccacccgcc tgaagcgcac cgcccgccgc
     3481 cgctacaccc gccgcaagaa ccgcatctgc tacctgcagg agatcttcag caacgagatg
     3541 gccaaggtgg acgacagctt cttccaccgc ctggaggaga gcttcctggt ggaggaggac
     3601 aagaagcacg agcgccaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag
     3661 aagtacccca ccatctacca tctgcgcaag aagctggtgg acagcaccga caaggccgac
     3721 ctgcgcctga tctacctggc cctggcccac atgatcaagt tccgcggcca cttcctgatc
     3781 gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag
     3841 acctacaacc agctgttcga ggagaacccc atcaacgcca gcggcgtgga cgccaaggcc
     3901 atcctgagcg cccgcctgag caagagccgc cgcctggaga acctgatcgc ccagctgccc
     3961 ggcgagaaga agaacggcct gttcggcaac ctgatcgccc tgagcctggg cctgaccccc
     4021 aacttcaaga gcaacttcga cctggccgag gacgccaagc tgcagctgag caaggacacc
     4081 tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttc
     4141 ctggccgcca agaacctgag cgacgccatc ctgctgagcg acatcctgcg cgtgaacacc
     4201 gagatcacca aggcccccct gagcgccagc atgatcaagc gctacgacga gcaccaccag
     4261 gacctgaccc tgctgaaggc cctggtgcgc cagcagctgc ccgagaagta caaggagatc
     4321 ttcttcgacc agagcaagaa cggctacgcc ggctacatcg acggcggcgc cagccaggag
     4381 gagttctaca agttcatcaa gcccatcctg gagaagatgg acggcaccga ggagctgctg
     4441 gtgaagctga accgcgagga cctgctgcgc aagcagcgca ccttcgacaa cggcagcatc
     4501 ccccaccaga tccacctggg cgagctgcac gccatcctgc gccgccagga ggacttctac
     4561 cccttcctga aggacaaccg cgagaagatc gagaagatcc tgaccttccg catcccctac
     4621 tacgtgggcc ccctggcccg cggcaacagc cgcttcgcct ggatgacccg caagagcgag
     4681 gagaccatca ccccctggaa cttcgaggag gtggtggaca agggcgccag cgcccagagc
     4741 ttcatcgagc gcatgaccaa cttcgacaag aacctgccca acgagaaggt gctgcccaag
     4801 cacagcctgc tgtacgagta cttcaccgtg tacaacgagc tgaccaaggt gaagtacgtg
     4861 accgagggca tgcgcaagcc cgccttcctg agcggcgagc agaagaaggc catcgtggac
     4921 ctgctgttca agaccaaccg caaggtgacc gtgaagcagc tgaaggagga ctacttcaag
     4981 aagatcgagt gcttcgacag cgtggagatc agcggcgtgg aggaccgctt caacgccagc
     5041 ctgggcacct accacgacct gctgaagatc atcaaggaca aggacttcct ggacaacgag
     5101 gagaacgagg acatcctgga ggacatcgtg ctgaccctga ccctgttcga ggaccgcgag
     5161 atgatcgagg agcgcctgaa gacctacgcc cacctgttcg acgacaaggt gatgaagcag
     5221 ctgaagcgcc gccgctacac cggctggggc cgcctgagcc gcaagcttat caacggcatc
     5281 cgcgacaagc agagcggcaa gaccatcctg gacttcctga agagcgacgg cttcgccaac
     5341 cgcaacttca tgcagctgat ccacgacgac agcctgacct tcaaggagga catccagaag
     5401 gcccaggtga gcggccaggg cgacagcctg cacgagcaca tcgccaacct ggccggcagc
     5461 cccgccatca agaagggcat cctgcagacc gtgaaggtgg tggacgagct ggtgaaggtg
     5521 atgggccgcc acaagcccga gaacatcgtg atcgagatgg cccgcgagaa ccagaccacc
     5581 cagaagggcc agaagaacag ccgcgagcgc atgaagcgca tcgaggaggg catcaaggag
     5641 ctgggcagcc agatcctgaa ggagcacccc gtggagaaca cccagctgca gaacgagaag
     5701 ctgtacctgt actacctgca gaacggccgc gacatgtacg tggaccagga gctggacatc
     5761 aaccgcctga gcgactacga cgtggaccac atcgtgcccc agagcttcct gaaggacgac
     5821 agcatcgaca acaaggtgct gacccgcagc gacaagaacc gcggcaagag cgacaacgtg
     5881 cccagcgagg aggtggtgaa gaagatgaag aactactggc gccagctgct gaacgccaag
     5941 ctgatcaccc agcgcaagtt cgacaacctg accaaggccg agcgcggcgg cctgagcgag
     6001 ctggacaagg ccggcttcat caagcgccag ctggtggaga cccgccagat caccaagcac
     6061 gtggcccaga tcctggacag ccgcatgaac accaagtacg acgagaacga caagctgatc
     6121 cgcgaggtga aggtgatcac cctgaagagc aagctggtga gcgacttccg caaggacttc
     6181 cagttctaca aggtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctgaac
     6241 gccgtggtgg gcaccgccct gatcaagaag taccccaagc tggagagcga gttcgtgtac
     6301 ggcgactaca aggtgtacga cgtgcgcaag atgatcgcca agagcgagca ggagatcggc
     6361 aaggccaccg ccaagtactt cttctacagc aacatcatga acttcttcaa gaccgagatc
     6421 accctggcca acggcgagat ccgcaagcgc cccctgatcg agaccaacgg cgagaccggc
     6481 gagatcgtgt gggacaaggg ccgcgacttc gccaccgtgc gcaaggtgct gagcatgccc
     6541 caggtgaaca tcgtgaagaa gaccgaggtg cagaccggcg gcttcagcaa ggagagcatc
     6601 ctgcccaagc gcaacagcga caagctgatc gcccgcaaga aggactggga ccccaagaag
     6661 tacggcggct tcgacagccc caccgtggcc tacagcgtgc tggtggtggc caaggtggag
     6721 aagggcaaga gcaagaagct gaagagcgtg aaggagctgc tgggcatcac catcatggag
     6781 cgcagcagct tcgagaagaa ccccatcgac ttcctggagg ccaagggcta caaggaggtg
     6841 aagaaggacc tgatcatcaa gctgcccaag tacagcctgt tcgagctgga gaacggccgc
     6901 aagcgcatgc tggccagcgc cggcgagctg cagaagggca acgagctggc cctgcccagc
     6961 aagtacgtga acttcctgta cctggccagc cactacgaga agctgaaggg cagccccgag
     7021 gacaacgagc agaagcagct gttcgtggag cagcacaagc actacctgga cgagatcatc
     7081 gagcagatca gcgagttcag caagcgcgtg atcctggccg acgccaacct ggacaaggtg
     7141 ctgagcgcct acaacaagca ccgcgacaag cccatccgcg agcaggccga gaacatcatc
     7201 cacctgttca ccctgaccaa cctgggcgcc cccgccgcct tcaagtactt cgacaccacc
     7261 atcgaccgca agcgctacac cagcaccaag gaggtgctgg acgccaccct gatccaccag
     7321 agcatcaccg gtctgtacga gacccgcatc gacctgagcc agctgggcgg cgactctggt
     7381 ggttctccaa agaaaaagag aaaagtataa aagggtggga tatgaagatg aagatgaaat
     7441 atttggtgtg tcaaataaaa agcttgtgtg cttaagtttg tgtttttttc ttggcttgtt
     7501 gtgttatgaa tttgtggctt tttctaatat taaatgaatg taagatctca ttataatgaa
     7561 taaacaaatg tttctataat ccattgtgaa tgttttgttg gatctcttct gcagcatata
     7621 actactgtat gtgctatggt atggactatg gaatatgatt aaagataagg cgcgccgacc
     7681 cagctttctt gtacaaagtg gtgatatcac tagtgtatgt aggtatagta acatgaacaa
     7741 gaacagactc aagtacaaga tcgcatacga aaatggaaat ggcaatgtca cttccacata
     7801 atcaaacacg aatcctcata tcaacaaggc ctgagattct aactagctca taacaactta
     7861 gccaatagtt acttgagact accaaatgta tgtagaacta aagactaagg gacagagagt
     7921 tcgtctaaac aggtgaatct agtcgttgtt atctaataaa caattcagcc ccaaatgcag
     7981 aacacacata gagctctcta ttgattcaaa ttacgatctg atactgataa cgtctagatt
     8041 tttagggtta aagcaatcaa tcacctgacg attcaaggtg gttggatcat gacgattcca
     8101 gaaaacatca agcaagctct caaagctaca ctctttggga tcatactgaa ctctaacaac
     8161 ctcgttatgt cccgtagtgc cagtacagac atcctcgtaa ctcggattgt gcacgatgcc
     8221 atggctatac ccaacctcgg tcttggtcac accaggaact ctctggtaag ctagctccac
     8281 tccccagaaa caaccggcgc caaattgcgc gaattgctga cctgaagacg gaacatcatc
     8341 gtcgggtcct tgggcgattg cggcggaaga tgggtcagct tgggcttgag gacgagaccc
     8401 gaatccgagt ctgttgaaaa ggttgttcat tggggatttg tatacggaga ttggtcgtcg
     8461 agaggtttga gggaaaggac aaatgggttt ggctctggag aaagagagtg cggctttaga
     8521 gagagaattg agaggtttag agagagatgc ggcggcgatg agcggaggag agacgacgag
     8581 gacctgcatt atcaaagcag tgacgtggtg aaatttggaa cttttaagag gcagatagat
     8641 ttattatttg tatccatttt cttcattgtt ctagaatgtc gcggaacaaa ttttaaaact
     8701 aaatcctaaa tttttctaat tttgttgcca atagtggata tgtgggccgt atagaaggaa
     8761 tctattgaag gcccaaaccc atactgacga gcccaaaggt tcgttttgcg ttttatgttt
     8821 cggttcgatg ccaacgccac attctgagct aggcaaaaaa caaacgtgtc tttgaataga
     8881 ctcctctcgt taacacatgc agcggctgca tggtgacgcc attaacacgt ggcctacaat
     8941 tgcatgatgt ctccattgac acgtgacttc tcgtctcctt tcttaatata tctaacaaac
     9001 actcctacct cttccaaaat atatacacat ctttttgatc aatctctcat tcaaaatctc
     9061 attctctcta gtaaacaaga acaaaaaaat ggcggataca gctagaggaa cccatcacga
     9121 tatcatcggc agagaccagt acccgatgat gggccgagac cgagaccagt accagatgtc
     9181 cggacgagga tctgactact ccaagtctag gcagattgct aaagctgcaa ctgctgtcac
     9241 agctggtggt tccctccttg ttctctccag ccttaccctt gttggaactg tcatagcttt
     9301 gactgttgca acacctctgc tcgttatctt cagcccaatc cttgtcccgg ctctcatcac
     9361 agttgcactc ctcatcaccg gttttctttc ctctggaggg tttggcattg ccgctataac
     9421 cgttttctct tggatttaca agtaagcaca catttatcat cttacttcat aattttgtgc
     9481 aatatgtgca tgcatgtgtt gagccagtag ctttggatca atttttttgg tcgaataaca
     9541 aatgtaacaa taagaaattg caaattctag ggaacatttg gttaactaaa tacgaaattt
     9601 gacctagcta gcttgaatgt gtctgtgtat atcatctata taggtaaaat gcttggtatg
     9661 atacctattg attgtgaata ggtacgcaac gggagagcac ccacagggat cagacaagtt
     9721 ggacagtgca aggatgaagt tgggaagcaa agctcaggat ctgaaagaca gagctcagta
     9781 ctacggacag caacatactg gtggggaaca tgaccgtgac cgtactcgtg gtggccagca
     9841 cactactatg agcgagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac
     9901 cgtgaacaac caccacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac
     9961 ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct
    10021 ggctaccagc ttcatgtacg gcagcagaac cttcatcaac cacacccagg gcatccccga
    10081 cttctttaag cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga
    10141 cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa
    10201 cgtcaagatc agaggggtga acttcccatc caacggccct gtgatgcaga agaaaacact
    10261 cggctgggag gccaacaccg agatgctgta ccccgctgac ggcggcctgg aaggcagaag
    10321 cgacatggcc ctgaagctcg tgggcggggg ccacctgatc tgcaacttca agaccacata
    10381 cagatccaag aaacccgcta agaacctcaa gatgcccggc gtctactatg tggaccacag
    10441 actggaaaga atcaaggagg ccgacaaaga gacctacgtc gagcagcacg aggtggctgt
    10501 ggccagatac tgcgacctcc ctagcaaact ggggcacaag tgaatcgttc aaacatttgg
    10561 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
    10621 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
    10681 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
    10741 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcactag
    10801 tgcggccgcc tgcaggtcga ccatatggga gagctcaagc ttagcttgag cttggatcag
    10861 attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta
    10921 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
    10981 ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt
    11041 actttaaagt actttaaagt actttaaagt actttgatcc aacccctccg ctgctatagt
    11101 gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc
    11161 taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt
    11221 agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc
    11281 gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac
    11341 caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc
    11401 accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt
    11461 gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc
    11521 gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc
    11581 accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt
    11641 tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag
    11701 tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac
    11761 caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg
    11821 taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc
    11881 ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa
    11941 gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag
    12001 agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa
    12061 ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg
    12121 ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt
    12181 aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac
    12241 gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac
    12301 gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt
    12361 cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct
    12421 aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg
    12481 cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa
    12541 ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc
    12601 cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc
    12661 ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct
    12721 ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg
    12781 cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga
    12841 ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag
    12901 aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag
    12961 gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg
    13021 ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc
    13081 gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag
    13141 tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga
    13201 cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt
    13261 tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgagcggt
    13321 cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag
    13381 ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa
    13441 tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt
    13501 gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg
    13561 ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg
    13621 tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta
    13681 gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg
    13741 gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc
    13801 ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc
    13861 ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc
    13921 atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc
    13981 ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc
    14041 gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg
    14101 gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca
    14161 cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt
    14221 ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat
    14281 gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg
    14341 cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg
    14401 ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc
    14461 acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca
    14521 aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc
    14581 gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt
    14641 gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg
    14701 ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa
    14761 atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc
    14821 gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa
    14881 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
    14941 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
    15001 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt
    15061 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
    15121 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
    15181 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
    15241 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    15301 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    15361 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    15421 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    15481 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    15541 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    15601 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    15661 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    15721 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
    15781 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    15841 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    15901 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    15961 taagggattt tggtcatgca tgatatatct cccaatttgt gtagggctta ttatgcacgc
    16021 ttaaaaataa taaaagcaga cttgacctga tagtttggct gtgagcaatt atgtgcttag
    16081 tgcatctaac gcttgagtta agccgcgccg cgaagcggcg tcggcttgaa cgaatttcta
    16141 gctagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg gacaaattct
    16201 tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata agcctgtcta
    16261 gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg
    16321 acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc
    16381 actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt taaggtttca
    16441 tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga
    16501 cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg
    16561 atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc
    16621 agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact
    16681 tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg
    16741 atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata
    16801 tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac
    16861 gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg
    16921 gcgatcaccg cttcccccat gatgtttaac tttgttttag ggcgactgcc ctgctgcgta
    16981 acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg
    17041 gatgcccgag gcatagactg taccccaaaa aaacagtcat aacaagccat gaaaaccgcc
    17101 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata
    17161 cgctacttgc attacagctt acgaaccgaa caggcttatg tccactgggt tcgtgcccga
    17221 attgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga
    17281 tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat
    17341 gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct
    17401 gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
    17461 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
    17521 gacgttttta atgtactgaa ttaacgccga attgaattat cagcttgcat gccggtcgat
    17581 ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt
    17641 tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa
    17701 ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata
    17761 tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt
    17821 ttgaacgatc tgcttgactc tagggaatta attcctgaat cactgcgacc ggccctcccg
    17881 cgacccagcc gagcgagctt agcgaactgt ggacgagaac tgtgccacca agcgtaaggc
    17941 cgttctctcg catttgcctt gctaggctcg cgcgagttgc tggctgaggc gttctcgaaa
    18001 tcagctcttg ttcggtcggc atctactcta ttcctttgcc ctcggacgag tgctggggcg
    18061 tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct
    18121 gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg
    18181 accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc
    18241 aagaccaatg cggagcatat acgcccggag ccttggcgat cctgcaagct ccggatgcct
    18301 ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat
    18361 gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt
    18421 tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg
    18481 gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc
    18541 actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca
    18601 tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc
    18661 gctcgtctgg ctaagatcgg ccgcagcgat agcatccata gcctccgcga ccggctgaag
    18721 aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg cacggcggga
    18781 gatgcaatag gtcaggctct cgctgaactc cccaatgtca agcacttccg gaatcgggag
    18841 cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct
    18901 atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc
    18961 gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc
    19021 gacagacgtc gcggtgagtt caggcttttt catagggggg attcgagttg agagtgaata
    19081 tgagactcta attggatacc gaggggaatt tatggaacgt cagtggagca tttttgacaa
    19141 gaaatatttg ctagctgata gtgaccttag gcgacttttg aacgcgcaat aatggtttct
    19201 gacgtatgtg cttagctcat taaactccag aaacccgcgg ctcagtggct ccttcaacgt
    19261 tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata
    19321 acgtgactcc cttaattctc cgctcatgat cgataattcg cggtacccgg ggatcctcta
    19381 gagggcccga cgtcgcatgc tcccggccgc catggccgcg ggatatcaca agtttgtaca
    19441 aaaaagcagg ctccgcggcc gctcgttgaa caacggaaac tcgacttgcc ttccgcacaa
    19501 tacatcattt cttcttagct ttttttcttc ttcttcgttc atacagtttt tttttgttta
    19561 tcagcttaca ttttcttgaa ccgtagcttt cgttttcttc tttttaac
//
