LOCUS       sequence-22188-e         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  TITLE     GFP plasmids
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCS2 mt-GFP (BamHI minus)
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             27..56
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             66..95
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             105..134
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             144..173
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             183..212
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             246..275
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(318..346)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ancgatcccn tcgatttaaa gctatggagc aaaagctcat ttctgaagag gacttgaatg
       61 aaatggagca aaagctcatt tctgaagagg acttgaatga aatggagcaa aagctcattt
      121 ctgaagagga cttgaatgaa atggagcaaa agctcatttc tgaagaggac ttgaatgaaa
      181 tggagcaaaa gctcatttct gaagaggact tgaatgaaat ggagagcttg ggcgacctca
      241 ccatggagca aaagctcatt tctgaagagg acttgaattc ctctagaatg agtaaaggag
      301 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
      361 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
      421 aatttatttg cactactgga aaactacctg ttccatggcc cacacttgtc actactttca
      481 cttatggtgt tcaatgcttt tcaagatacc cagatcatat gaaacggcat gactttttca
      541 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
      601 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
      661 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
      721 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact
      781 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
      841 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
      901 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt ga
//
