LOCUS       sequence-22195-e         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Takahashi K, Mitsui K, Yamanaka S
  TITLE     Role of ERas in promoting tumour-like properties in mouse embryonic 
            stem cells.
  JOURNAL   Nature. 2003 May 29. 423(6939):541-5.
  PUBMED    12774123
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2003 May 29. 423(6939):541-5."
COMMENT     Sequence Label: pPyCAG-Myc-ERas-IP
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             63..92
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             102..131
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             141..170
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             180..209
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             243..272
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    282..306
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aattcctcga cgatttaagc tatggagcaa agctcatttc tgaagaggac ttgaatgaaa
       61 tggagcaaaa gctcatttct gaagaggact tgaatgaaat ggagcaaaag ctcatttctg
      121 aagaggactt gaatgaaatg gagcaaaagc tcatttctga agaggacttg aatgaaatgg
      181 agcaaaagct catttctgaa gaggacttga atgaaatgga gagcttgggc gacctcacca
      241 tggagcaaaa gctcatttct gaagaggact tgaattatca aacaagtttg tacaaaaaag
      301 caggctgggg aatggctttg cctacaaagt ctagcatctt ggacctgagc tccggcaccc
      361 catgcaccag atctccagag gaaagtcacg aggcttgggc acagtgcaaa gatgctggca
      421 ggcagctacc cgagtacaag gcagtggtgg tgggtgcaag tggtgttggt aaaagtgctc
      481 tcaccatcca gatgactcac caatgcttcg tgaaagacca tgaccccact atccaagatt
      541 cctactggaa ggaagtggcc agggacaacg gaggctacat tctaaatgtt ctggatacat
      601 ctgggcagga tattcaccgg gctctgcgtg accagtgctt ggcatctggt gatggtgtgc
      661 tgggcgtctt tgctcttgac gacccctcgt ctctggacca gttgcagcag atatggtcca
      721 cctggacccc tcaccacaag cagcctctgg tactagtgg
//
