LOCUS       sequence-221954-        5492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  evaluate FOXA1 DIV motif enhancer activity in SNP rs281038 minor 
            allele (G).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Wang X, Srivastava Y, Jankowski A, Malik V, Wei Y, Del Rosario RC, 
            Cojocaru V, Prabhakar S, Jauch R
  TITLE     DNA-mediated dimerization on a compact sequence signature controls 
            enhancer engagement and regulation by FOXA1.
  JOURNAL   Nucleic Acids Res. 2018 Jun 20;46(11):5470-5486. doi: 
            10.1093/nar/gky259.
  PUBMED    29669022
REFERENCE   2  (bases 1 to 5492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2018-06-20- 20"; volume: "46"; issue: 
            "11"; pages: "5470-5486"
COMMENT     Sequence Label: rs281-minor
FEATURES             Location/Qualifiers
     source          1..5492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..752
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      782..791
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             788..2440
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGEVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2484..2605
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2495..2514)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2549..2568
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2853..2870)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3024..3612)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3104..3123)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3812..4603,4604..4672))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4777..4825
                     /note="synthetic polyadenylation signal"
     misc_feature    4839..4930
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4879..4898
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 aatgagtctt cggacctcgc gggggccgct taagcggtgg ttagggtttg tctgacgcgg
       61 ggggaggggg aaggaacgaa acactctcat tcggaggcgg ctcggggttt ggtcttggtg
      121 gccacgggca cgcagaagag cgccgcgatc ctcttaagca cccccccgcc ctccgtggag
      181 gcgggggttt ggtcggcggg tggtaactgg cgggccgctg actcgggcgg gtcgcgcgcc
      241 ccagagtgtg accttttcgg tctgctcgca gacccccggg cggcgccgcc gcggcggcga
      301 cgggctcgct gggtcctagg ctccatgggg accgtatacg tggacaggct ctggagcatc
      361 cgcacgactg cggtgatatt accggagacc ttctgcggga cgagccgggt cacgcggctg
      421 acgcggagcg tccgttgggc gacaaacacc aggacggggc acaggtacac tatcttgtca
      481 cccggaggcg cgagggactg caggagcttc agggagtggc gcagctgctt catccccgtg
      541 gcccgttgct cgcgtttgct ggcggtgtcc ccggaagaaa tatatttgca tgtctttagt
      601 tctatgatga cacaaacccc gcccagcgtc ttgtcattgg cgaattcgaa cacgcagatg
      661 cagtcggggc ggcgcggtcc caggtccact tcgcatatta aggtgacgcg tgtggcctcg
      721 aacaccgagc gaccctgcag cgacccgctt aaaagcttgg caatccggta ctgttggtaa
      781 agccaccatg gaagatgcca aaaacattaa gaagggccca gcgccattct acccactcga
      841 agacgggacc gccggcgagc agctgcacaa agccatgaag cgctacgccc tggtgcccgg
      901 caccatcgcc tttaccgacg cacatatcga ggtggacatt acctacgccg agtacttcga
      961 gatgagcgtt cggctggcag aagctatgaa gcgctatggg ctgaatacaa accatcggat
     1021 cgtggtgtgc agcgagaata gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat
     1081 cggtgtggct gtggccccag ctaacgacat ctacaacgag cgcgagctgc tgaacagcat
     1141 gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa
     1201 cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc atggatagca agaccgacta
     1261 ccagggcttc caaagcatgt acaccttcgt gacttcccat ttgccacccg gcttcaacga
     1321 gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga tcatgaacag
     1381 tagtggcagt accggattgc ccaagggcgt agccctaccg caccgcaccg cttgtgtccg
     1441 attcagtcat gcccgcgacc ccatcttcgg caaccagatc atccccgaca ccgctatcct
     1501 cagcgtggtg ccatttcacc acggcttcgg catgttcacc acgctgggct acttgatctg
     1561 cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc gcagcttgca
     1621 agactataag attcaatctg ccctgctggt gcccacacta tttagcttct tcgctaagag
     1681 cactctcatc gacaagtacg acctaagcaa cttgcacgag atcgccagcg gcggggcgcc
     1741 gctcagcaag gaggtaggtg aggccgtggc caaacgcttc cacctaccag gcatccgcca
     1801 gggctacggc ctgacagaaa caaccagcgc cattctgatc acccccgaag gggacgacaa
     1861 gcctggcgca gtaggcgagg tggtgccctt cttcgaggct aaggtggtgg acttggacac
     1921 cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat
     1981 gagcggctac gttaacaacc ccgaggctac aaacgctctc atcgacaagg acggctggct
     2041 gcacagcggc gacatcgcct actgggacga ggacgagcac ttcttcatcg tggaccggct
     2101 gaagagcctg atcaaataca agggctacca ggtagcccca gccgaactgg agagcatcct
     2161 gctgcaacac cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg
     2221 cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa accatgaccg agaaggagat
     2281 cgtggactat gtggccagcc aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt
     2341 cgtggacgag gtgcctaaag gactgaccgg caagttggac gcccgcaaga tccgcgagat
     2401 tctcattaag gccaagaagg gcggcaagat cgccgtgtaa taattctaga gtcggggcgg
     2461 ccggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag
     2521 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     2581 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     2641 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat
     2701 cgataaggat ccgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
     2761 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
     2821 taggacaggt gccggcagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     2881 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2941 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3001 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3061 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3121 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3181 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3241 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3301 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3361 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3421 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3481 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3541 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3601 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3661 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3721 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3781 agcggccgca aatgctaaac cactgcagtg gttaccagtg cttgatcagt gaggcaccga
     3841 tctcagcgat ctgcctattt cgttcgtcca tagtggcctg actccccgtc gtgtagatca
     3901 ctacgattcg tgagggctta ccatcaggcc ccagcgcagc aatgatgccg cgagagccgc
     3961 gttcaccggc ccccgatttg tcagcaatga accagccagc agggagggcc gagcgaagaa
     4021 gtggtcctgc tactttgtcc gcctccatcc agtctatgag ctgctgtcgt gatgctagag
     4081 taagaagttc gccagtgagt agtttccgaa gagttgtggc cattgctact ggcatcgtgg
     4141 tatcacgctc gtcgttcggt atggcttcgt tcaactctgg ttcccagcgg tcaagccggg
     4201 tcacatgatc acccatatta tgaagaaatg cagtcagctc cttagggcct ccgatcgttg
     4261 tcagaagtaa gttggccgcg gtgttgtcgc tcatggtaat ggcagcacta cacaattctc
     4321 ttaccgtcat gccatccgta agatgctttt ccgtgaccgg cgagtactca accaagtcgt
     4381 tttgtgagta gtgtatacgg cgaccaagct gctcttgccc ggcgtctata cgggacaaca
     4441 ccgcgccaca tagcagtact ttgaaagtgc tcatcatcgg gaatcgttct tcggggcgga
     4501 aagactcaag gatcttgccg ctattgagat ccagttcgat atagcccact cttgcaccca
     4561 gttgatcttc agcatctttt actttcacca gcgtttcggg gtgtgcaaaa acaggcaagc
     4621 aaaatgccgc aaagaaggga atgagtgcga cacgaaaatg ttggatgctc atactcgtcc
     4681 tttttcaata ttattgaagc atttatcagg gttactagta cgtctctcaa ggataagtaa
     4741 gtaatattaa ggtacgggag gtattggaca ggccgcaata aaatatcttt attttcatta
     4801 catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac
     4861 aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga
     4921 acatttctct ggcctaactg gccggtaccc cgcattcttt tcggggtttc aacttcttcc
     4981 tgattcaacc ttgggaggtt gtgtatttcc atgaatttat ccatttcctc tagatttcct
     5041 agtttgtgtg catagaggtg ttcataatag tctttgagga tcttttgtat ttctgtgggg
     5101 tagcttgtaa tgtcaccttt gtcatgaaaa cataaaaatg ttttcttacg taattctaaa
     5161 gcttgctttt tttttttttt tgcccctgtt gttcaaactg tggtagcccg gactgagtca
     5221 gggacctaga atgtcataat ctacctcctt catccttttg aaatttttta aaatggtgtt
     5281 ttataggagt atctctgacc aattgaaaag atgaccaatt attgatagat tagcccagac
     5341 cagtgtgtct gtgaatgttt tatatttgtc atttcatggt gccacatatt actccatgct
     5401 attgattgct attgaccttg gatattgtgt aaattgttaa tgaacttggt gactgactct
     5461 tctggtctct gactttagca ctgggccgat ta
//
