LOCUS       sequence-221973-       15334 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15334)
  AUTHORS   Osakada F, Mori T, Cetin AH, Marshel JH, Virgen B, Callaway EM
  TITLE     New rabies virus variants for monitoring and manipulating activity 
            and gene expression in defined neural circuits.
  JOURNAL   Neuron. 2011 Aug 25;71(4):617-31.
  PUBMED    21867879
REFERENCE   2  (bases 1 to 15334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2011-08-25"; volume: "71(4)"; pages: "617-31"
COMMENT     Sequence Label: pSADdeltaG-F3
FEATURES             Location/Qualifiers
     source          1..15334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        407..610
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     561..581
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     655..674
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        655..673
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5865..5876
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10974..10991)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10980..11204
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11250..11678
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11337..11356)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11547..11568
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11687..11707)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11692..12021
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11872..12007
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11934..11953
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        12069..12116
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12135..12509
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12639..12760
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12676..12695)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12730..12749
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12809..12825)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12809..12825)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12822..12844)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12833..12849
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12857..12863,12864..12881,12882..12887))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12902..12923
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(13371..13959)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13451..13470)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14130..14921,14922..14990))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14753..14772
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14991..15095)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(15170..15189)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
      661 gactcactat agggagaccc aagctggcta gattaagcgt ctgatgagtc cgtgaggacg
      721 aaacccggag tcccgggtca cgcttaacaa ccagatcaaa gaaaaaacag acattgtcaa
      781 ttgcaaagca aaaatgtaac acccctacaa tggatgccga caagattgta ttcaaagtca
      841 ataatcaggt ggtctctttg aagcctgaga ttatcgtgga tcaatatgag tacaagtacc
      901 ctgccatcaa agatttgaaa aagccctgta taaccctagg aaaggctccc gatttaaata
      961 aagcatacaa gtcagttttg tcaggcatga gcgccgccaa acttaatcct gacgatgtat
     1021 gttcctattt ggcagcggca atgcagtttt ttgaggggac atgtccggaa gactggacca
     1081 gctatggaat tgtgattgca cgaaaaggag ataagatcac cccaggttct ctggtggaga
     1141 taaaacgtac tgatgtagaa gggaattggg ctctgacagg aggcatggaa ctgacaagag
     1201 accccactgt ccctgagcat gcgtccttag tcggtcttct cttgagtctg tataggttga
     1261 gcaaaatatc cgggcaaaac actggtaact ataagacaaa cattgcagac aggatagagc
     1321 agatttttga gacagcccct tttgttaaaa tcgtggaaca ccatactcta atgacaactc
     1381 acaaaatgtg tgctaattgg agtactatac caaacttcag atttttggcc ggaacctatg
     1441 acatgttttt ctcccggatt gagcatctat attcagcaat cagagtgggc acagttgtca
     1501 ctgcttatga agactgttca ggactggtat catttactgg gttcataaaa caaatcaatc
     1561 tcaccgctag agaggcaata ctatatttct tccacaagaa ctttgaggaa gagataagaa
     1621 gaatgtttga gccagggcag gagacagctg ttcctcactc ttatttcatc cacttccgtt
     1681 cactaggctt gagtgggaaa tctccttatt catcaaatgc tgttggtcac gtgttcaatc
     1741 tcattcactt tgtaggatgc tatatgggtc aagtcagatc cctaaatgca acggttattg
     1801 ctgcatgtgc tcctcatgaa atgtctgttc tagggggcta tctgggagag gaattcttcg
     1861 ggaaagggac atttgaaaga agattcttca gagatgagaa agaacttcaa gaatacgagg
     1921 cggctgaact gacaaagact gacgtagcac tggcagatga tggaactgtc aactctgacg
     1981 acgaggacta cttttcaggt gaaaccagaa gtccggaggc tgtttatact cgaatcatga
     2041 tgaatggagg tcgactaaag agatctcaca tacggagata tgtctcagtc agttccaatc
     2101 atcaagcccg tccaaactca ttcgccgagt ttctaaacaa gacatattcg agtgactcat
     2161 aagaagttga ataacaaaat gccggaaatc tacggattgt gtatatccat catgaaaaaa
     2221 actaacaccc ctcctttcga accatcccaa acatgagcaa gatctttgtc aatcctagtg
     2281 ctattagagc cggtctggcc gatcttgaga tggctgaaga aactgttgat ctgatcaata
     2341 gaaatatcga agacaatcag gctcatctcc aaggggaacc catagaggtg gacaatctcc
     2401 ctgaggatat ggggcgactt cacctggatg atggaaaatc gcccaaccat ggtgagatag
     2461 ccaaggtggg agaaggcaag tatcgagagg actttcagat ggatgaagga gaggatccta
     2521 gcttcctgtt ccagtcatac ctggaaaatg ttggagtcca aatagtcaga caaatgaggt
     2581 caggagagag atttctcaag atatggtcac agaccgtaga agagattata tcctatgtcg
     2641 cggtcaactt tcccaaccct ccaggaaagt cttcagagga taaatcaacc cagactactg
     2701 gccgagagct caagaaggag acaacaccca ctccttctca gagagaaagc caatcatcga
     2761 aagccaggat ggcggctcaa attgcttctg gccctccagc ccttgaatgg tcggctacca
     2821 atgaagagga tgatctatca gtggaggctg agatcgctca ccagattgca gaaagtttct
     2881 ccaaaaaata taagtttccc tctcgatcct cagggatact cttgtataat tttgagcaat
     2941 tgaaaatgaa ccttgatgat atagttaaag aggcaaaaaa tgtaccaggt gtgacccgtt
     3001 tagcccatga cgggtccaaa ctccccctaa gatgtgtact gggatgggtc gctttggcca
     3061 actctaagaa attccagttg ttagtcgaat ccgacaagct gagtaaaatc atgcaagatg
     3121 acttgaatcg ctatacatct tgctaaccga acctctcccc tcagtccctc tagacaataa
     3181 aatccgagat gtcccaaagt caacatgaaa aaaacaggca acaccactga taaatcgatg
     3241 aacctcctac gtaagatagt gaaaaaccgc agggacgagg acactcaaaa atcctctccc
     3301 gcgtcagccc ctctggatga cgatgacttg tggcttccac cccctgaata cgtcccgctg
     3361 aaagaactta caggcaagaa gaacatgagg aacttttgta tcaacggaag ggttaaagtg
     3421 tgtagcccga atggttactc gttcaggatc ctgcggcaca ttctgaaatc attcgacgag
     3481 atatattctg ggaatcatag gatgatcggg ttagtcaaag tggttattgg actggctttg
     3541 tcaggatctc cagtccctga gggcctgaac tgggtataca aattgaggag aacctttatc
     3601 ttccagtggg ctgattccag gggccctctt gaaggggagg agttggaata ctctcaggag
     3661 atcacttggg atgatgatac tgagttcgtc ggattgcaaa taagagtgat tgcaaaacag
     3721 tgtcatatcc agggcagagt ctggtgtatc aacatgaacc cgagagcatg tcaactatgg
     3781 tctgacatgt ctcttcagac acaaaggtcc gaagaggaca aagattcctc tctgcttcta
     3841 gaataacgta cgtcagatta tatcccgcaa atttatcact tgtttacctc tggaggagag
     3901 aacatatggg ctcaactcca acccttggga gcaatataac aaaaaacatg ttatggtgcc
     3961 attaaaccgc tgcatttcat caaagtcaag ttgattacct ttacattttg atcctcttgg
     4021 atgtgaaaaa aactattaac atccctcaaa ggacctgcag gtacgcggcc gctacgcccg
     4081 ggctacgcta gcatgaaaaa aactaacacc cctccttaat taatacggcg cgcctacccg
     4141 cggtagcttt tcagtcgaga aaaaaacatt agatcagaag aacaactggc aacacttctc
     4201 aacctgagac ttacttcaag atgctcgatc ctggagaggt ctatgatgac cctattgacc
     4261 caatcgagtt agaggctgaa cccagaggaa cccccattgt ccccaacatc ttgaggaact
     4321 ctgactacaa tctcaactct cctttgatag aagatcctgc tagactaatg ttagaatggt
     4381 taaaaacagg gaatagacct tatcggatga ctctaacaga caattgctcc aggtctttca
     4441 gagttttgaa agattatttc aagaaggtag atttgggttc tctcaaggtg ggcggaatgg
     4501 ctgcacagtc aatgatttct ctctggttat atggtgccca ctctgaatcc aacaggagcc
     4561 ggagatgtat aacagacttg gcccatttct attccaagtc gtcccccata gagaagctgt
     4621 tgaatctcac gctaggaaat agagggctga gaatcccccc agagggagtg ttaagttgcc
     4681 ttgagagggt tgattatgat aatgcatttg gaaggtatct tgccaacacg tattcctctt
     4741 acttgttctt ccatgtaatc accttataca tgaacgccct agactgggat gaagaaaaga
     4801 ccatcctagc attatggaaa gatttaacct cagtggacat cgggaaggac ttggtaaagt
     4861 tcaaagacca aatatgggga ctgctgatcg tgacaaagga ctttgtttac tcccaaagtt
     4921 ccaattgtct ttttgacaga aactacacac ttatgctaaa agatcttttc ttgtctcgct
     4981 tcaactcctt aatggtcttg ctctctcccc cagagccccg atactcagat gacttgatat
     5041 ctcaactatg ccagctgtac attgctgggg atcaagtctt gtctatgtgt ggaaactccg
     5101 gctatgaagt catcaaaata ttggagccat atgtcgtgaa tagtttagtc cagagagcag
     5161 aaaagtttag gcctctcatt cattccttgg gagactttcc tgtatttata aaagacaagg
     5221 taagtcaact tgaagagacg ttcggtccct gtgcaagaag gttctttagg gctctggatc
     5281 aattcgacaa catacatgac ttggtttttg tgtttggctg ttacaggcat tgggggcacc
     5341 catatataga ttatcgaaag ggtctgtcaa aactatatga tcaggttcac cttaaaaaaa
     5401 tgatagataa gtcctaccag gagtgcttag caagcgacct agccaggagg atccttagat
     5461 ggggttttga taagtactcc aagtggtatc tggattcaag attcctagcc cgagaccacc
     5521 ccttgactcc ttatatcaaa acccaaacat ggccacccaa acatattgta gacttggtgg
     5581 gggatacatg gcacaagctc ccgatcacgc agatctttga gattcctgaa tcaatggatc
     5641 cgtcagaaat attggatgac aaatcacatt ctttcaccag aacgagacta gcttcttggc
     5701 tgtcagaaaa ccgagggggg cctgttccta gcgaaaaagt tattatcacg gccctgtcta
     5761 agccgcctgt caatccccga gagtttctga ggtctataga cctcggagga ttgccagatg
     5821 aagacttgat aattggcctc aagccaaagg aacgggaatt gaagattgaa ggtcgattct
     5881 ttgctctaat gtcatggaat ctaagattgt attttgtcat cactgaaaaa ctcttggcca
     5941 actacatctt gccacttttt gacgcgctga ctatgacaga caacctgaac aaggtgttta
     6001 aaaagctgat cgacagggtc accgggcaag ggcttttgga ctattcaagg gtcacatatg
     6061 catttcacct ggactatgaa aagtggaaca accatcaaag attagagtca acagaggatg
     6121 tattttctgt cctagatcaa gtgtttggat tgaagagagt gttttctaga acacacgagt
     6181 tttttcaaaa ggcctggatc tattattcag acagatcaga cctcatcggg ttacgggagg
     6241 atcaaatata ctgcttagat gcgtccaacg gcccaacctg ttggaatggc caggatggcg
     6301 ggctagaagg cttacggcag aagggctgga gtctagtcag cttattgatg atagatagag
     6361 aatctcaaat caggaacaca agaaccaaaa tactagctca aggagacaac caggttttat
     6421 gtccgacata catgttgtcg ccagggctat ctcaagaggg gctcctctat gaattggaga
     6481 gaatatcaag gaatgcactt tcgatataca gagccgtcga ggaaggggca tctaagctag
     6541 ggctgatcat caagaaagaa gagaccatgt gtagttatga cttcctcatc tatggaaaaa
     6601 cccctttgtt tagaggtaac atattggtgc ctgagtccaa aagatgggcc agagtctctt
     6661 gcgtctctaa tgaccaaata gtcaacctcg ccaatataat gtcgacagtg tccaccaatg
     6721 cgctaacagt ggcacaacac tctcaatctt tgatcaaacc gatgagggat tttctgctca
     6781 tgtcagtaca ggcagtcttt cactacctgc tatttagccc aatcttaaag ggaagagttt
     6841 acaagattct gagcgctgaa ggggagagct ttctcctagc catgtcaagg ataatctatc
     6901 tagatccttc tttgggaggg atatctggaa tgtccctcgg aagattccat atacgacagt
     6961 tctcagaccc tgtctctgaa gggttatcct tctggagaga gatctggtta agctcccaag
     7021 agtcctggat tcacgcgttg tgtcaagagg ctggaaaccc agatcttgga gagagaacac
     7081 tcgagagctt cactcgcctt ctagaagatc cgaccacctt aaatatcaga ggaggggcca
     7141 gtcctaccat tctactcaag gatgcaatca gaaaggcttt atatgacgag gtggacaagg
     7201 tggaaaattc agagtttcga gaggcaatcc tgttgtccaa gacccataga gataatttta
     7261 tactcttctt aatatctgtt gagcctctgt ttcctcgatt tctcagtgag ctattcagtt
     7321 cgtctttttt gggaatcccc gagtcaatca ttggattgat acaaaactcc cgaacgataa
     7381 gaaggcagtt tagaaagagt ctctcaaaaa ctttagaaga atccttctac aactcagaga
     7441 tccacgggat tagtcggatg acccagacac ctcagagggt tgggggggtg tggccttgct
     7501 cttcagagag ggcagatcta cttagggaga tctcttgggg aagaaaagtg gtaggcacga
     7561 cagttcctca cccttctgag atgttgggat tacttcccaa gtcctctatt tcttgcactt
     7621 gtggagcaac aggaggaggc aatcctagag tttctgtatc agtactcccg tcctttgatc
     7681 agtcattttt ttcacgaggc cccctaaagg gatacttggg ctcgtccacc tctatgtcga
     7741 cccagctatt ccatgcatgg gaaaaagtca ctaatgttca tgtggtgaag agagctctat
     7801 cgttaaaaga atctataaac tggttcatta ctagagattc caacttggct caagctctaa
     7861 ttaggaacat tatgtctctg acaggccctg atttccctct agaggaggcc cctgtcttca
     7921 aaaggacggg gtcagccttg cataggttca agtctgccag atacagcgaa ggagggtatt
     7981 cttctgtctg cccgaacctc ctctctcata tttctgttag tacagacacc atgtctgatt
     8041 tgacccaaga cgggaagaac tacgatttca tgttccagcc attgatgctt tatgcacaga
     8101 catggacatc agagctggta cagagagaca caaggctaag agactctacg tttcattggc
     8161 acctccgatg caacaggtgt gtgagaccca ttgacgacgt gaccctggag acctctcaga
     8221 tcttcgagtt tccggatgtg tcgaaaagaa tatccagaat ggtttctggg gctgtgcctc
     8281 acttccagag gcttcccgat atccgtctga gaccaggaga ttttgaatct ctaagcggta
     8341 gagaaaagtc tcaccatatc ggatcagctc aggggctctt atactcaatc ttagtggcaa
     8401 ttcacgactc aggatacaat gatggaacca tcttccctgt caacatatac ggcaaggttt
     8461 cccctagaga ctatttgaga gggctcgcaa ggggagtatt gataggatcc tcgatttgct
     8521 tcttgacaag aatgacaaat atcaatatta atagacctct tgaattggtc tcaggggtaa
     8581 tctcatatat tctcctgagg ctagataacc atccctcctt gtacataatg ctcagagaac
     8641 cgtctcttag aggagagata ttttctatcc ctcagaaaat ccccgccgct tatccaacca
     8701 ctatgaaaga aggcaacaga tcaatcttgt gttatctcca acatgtgcta cgctatgagc
     8761 gagagataat cacggcgtct ccagagaatg actggctatg gatcttttca gactttagaa
     8821 gtgccaaaat gacgtaccta tccctcatta cttaccagtc tcatcttcta ctccagaggg
     8881 ttgagagaaa cctatctaag agtatgagag ataacctgcg acaattgagt tctttgatga
     8941 ggcaggtgct gggcgggcac ggagaagata ccttagagtc agacgacaac attcaacgac
     9001 tgctaaaaga ctctttacga aggacaagat gggtggatca agaggtgcgc catgcagcta
     9061 gaaccatgac tggagattac agccccaaca agaaggtgtc ccgtaaggta ggatgttcag
     9121 aatgggtctg ctctgctcaa caggttgcag tctctacctc agcaaacccg gcccctgtct
     9181 cggagcttga cataagggcc ctctctaaga ggttccagaa ccctttgatc tcgggcttga
     9241 gagtggttca gtgggcaacc ggtgctcatt ataagcttaa gcctattcta gatgatctca
     9301 atgttttccc atctctctgc cttgtagttg gggacgggtc aggggggata tcaagggcag
     9361 tcctcaacat gtttccagat gccaagcttg tgttcaacag tcttttagag gtgaatgacc
     9421 tgatggcttc cggaacacat ccactgcctc cttcagcaat catgagggga ggaaatgata
     9481 tcgtctccag agtgatagat cttgactcaa tctgggaaaa accgtccgac ttgagaaact
     9541 tggcaacctg gaaatacttc cagtcagtcc aaaagcaggt caacatgtcc tatgacctca
     9601 ttatttgcga tgcagaagtt actgacattg catctatcaa ccggatcacc ctgttaatgt
     9661 ccgattttgc attgtctata gatggaccac tctatttggt cttcaaaact tatgggacta
     9721 tgctagtaaa tccaaactac aaggctattc aacacctgtc aagagcgttc ccctcggtca
     9781 cagggtttat cacccaagta acttcgtctt tttcatctga gctctacctc cgattctcca
     9841 aacgagggaa gtttttcaga gatgctgagt acttgacctc ttccaccctt cgagaaatga
     9901 gccttgtgtt attcaattgt agcagcccca agagtgagat gcagagagct cgttccttga
     9961 actatcagga tcttgtgaga ggatttcctg aagaaatcat atcaaatcct tacaatgaga
    10021 tgatcataac tctgattgac agtgatgtag aatcttttct agtccacaag atggttgatg
    10081 atcttgagtt acagagggga actctgtcta aagtggctat cattatagcc atcatgatag
    10141 ttttctccaa cagagtcttc aacgtttcca aacccctaac tgacccctcg ttctatccac
    10201 cgtctgatcc caaaatcctg aggcacttca acatatgttg cagtactatg atgtatctat
    10261 ctactgcttt aggtgacgtc cctagcttcg caagacttca cgacctgtat aacagaccta
    10321 taacttatta cttcagaaag caagtcattc gagggaacgt ttatctatct tggagttggt
    10381 ccaacgacac ctcagtgttc aaaagggtag cctgtaattc tagcctgagt ctgtcatctc
    10441 actggatcag gttgatttac aagatagtga agactaccag actcgttggc agcatcaagg
    10501 atctatccag agaagtggaa agacaccttc ataggtacaa caggtggatc accctagagg
    10561 atatcagatc tagatcatcc ctactagact acagttgcct gtgaaccgga tactcctgga
    10621 agcctgccca tgctaagact cttgtgtgat gtatcttgaa aaaaacaaga tcctaaatct
    10681 gaacctttgg ttgtttgatt gtttttctca tttttgttgt ttatttgtta agcgtgggtc
    10741 ggcatggcat ctccacctcc tcgcggtccg acctgggcat ccgaaggagg acgcacgtcc
    10801 actcggatgg ctaagggaga agggcgaatt ccagcacact ggcggccgtt actagtggat
    10861 ccgagctcgg taccgagctc ggatccacta gtccagtgtg gtggaattct gcagatatcc
    10921 agcacagtgg cggccgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac
    10981 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
    11041 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
    11101 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
    11161 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
    11221 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
    11281 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
    11341 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
    11401 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
    11461 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
    11521 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
    11581 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
    11641 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
    11701 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
    11761 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
    11821 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
    11881 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
    11941 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
    12001 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
    12061 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
    12121 tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg
    12181 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg
    12241 acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc
    12301 aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg
    12361 ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg
    12421 agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg
    12481 tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg
    12541 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
    12601 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
    12661 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    12721 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
    12781 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
    12841 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
    12901 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
    12961 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
    13021 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    13081 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    13141 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaggcgctc ttccgcttcc
    13201 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
    13261 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
    13321 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    13381 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    13441 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    13501 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    13561 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    13621 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    13681 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    13741 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    13801 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    13861 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    13921 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    13981 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    14041 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    14101 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    14161 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    14221 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    14281 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    14341 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    14401 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    14461 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    14521 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    14581 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    14641 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    14701 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    14761 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    14821 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    14881 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    14941 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    15001 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    15061 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    15121 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
    15181 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
    15241 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
    15301 atctgcttag ggttaggcgt tttgcgctgc ttcg
//
