LOCUS       sequence-221979-        9562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab7a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9562)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab7a
FEATURES             Location/Qualifiers
     source          1..9562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1386..1406)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1414..1430)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1446..1580
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1470..1489)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1524..1543
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2019..2206,2207..2218)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2252..2268)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2252..2268)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2305..2325)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2305..2323)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2344..2360)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2357..2379)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2368..2384
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2392..2398,2399..2416,2417..2422))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2437..2458
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2575..2592)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2746..3334)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2826..2845)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3505..4296,4297..4365))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4128..4147
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4366..4470)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4633..4654)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5003..5014,5015..5177)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5550..5566
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5586..5606
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt gggcgccacc atgacatcaa ggaagaaagt
      781 tcttctgaag gtgatcattc ttggagattc cggggttgga aaaacatctc taatgaacca
      841 gtatgtgaat aaaaagttca gtaatcagta taaagccact ataggagcag atttcctgac
      901 aaaagaagtg atggttgacg accggcttgt cacaatgcag atatgggaca cagcaggtca
      961 ggagcggttt cagtctctgg gtgttgcttt ctatcgtggt gctgactgct gcgtcctggt
     1021 gtttgacgtc actgccccga acaccttcaa gacactggac agctggaggg atgagtttct
     1081 gatccaggcc agtcctcgag acccagaaaa cttccctttc gtggtgctgg gcaacaaaat
     1141 cgatctggag aatagacagg taacaactaa gcgagcacaa gcttggtgtc agagtaagaa
     1201 caacatccca tattttgaga ccagtgcaaa ggaggccatc aacgtagagc aggcattcca
     1261 gaccatcgcg cgcaatgcac tcaaacagga aactgaagtg gagttataca acgagttccc
     1321 cgagccaatt aaactagacc ggaacgatag agccaaacca tcagcagaga cctgtagctg
     1381 ctgaccaact ttattataca tagttgataa ttcactggcc gtcgttttac ggtaccatcg
     1441 atgatgatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     1501 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     1561 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     1621 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
     1681 atgatcctct agatcagatc tgcgaagata cggccacggg tgctcttgat cctgtggctg
     1741 attttggact gtgctgctcg cagctgctga tgaatcacat acttcctcca ttttcttcca
     1801 ctgattgact gttataattt ccctaatttc caggtcaagg tgctgtgcat tgtggtaata
     1861 gatgtgacat gacgtcactt ccaaaggacc aatgaacatg tctgaccaat ttcatataat
     1921 gtgaaaacga ttttcatagg cagaataaat aacatttaaa ttaaactggg catcagcgca
     1981 attcaattgg tttggtaata gcaagggaaa atagaatgaa gtgatctcca aaaaataagt
     2041 actttttgac tgtaaataaa attgtaagga gtaaaaagta cttttttttc taaaaaaatg
     2101 taattaagta aaagtaaaag tattgatttt taattgtact caagtaaagt aaaaatcccc
     2161 aaaaataata cttaagtaca gtaatcaagt aaaattactc aagtacttta cacctctggt
     2221 tcttgacccc ctaccttcag caagcccagc agatccacta gttctagagc ggccgccacc
     2281 gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
     2341 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     2401 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     2461 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     2521 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     2581 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     2641 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     2701 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     2761 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2821 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     2881 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2941 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3001 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3061 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3121 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3181 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     3241 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3301 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3361 gtctgacgct cagtggaacg aaaactcacg ttaaggcatt ttggtcatga gattatcaaa
     3421 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     3481 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     3541 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     3601 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     3661 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     3721 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     3781 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     3841 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     3901 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     3961 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4021 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4081 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     4141 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     4201 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     4261 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     4321 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     4381 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     4441 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt
     4501 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     4561 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     4621 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     4681 aaagggcgaa aaaccgtcta tcagggcgat ggcccacgct ggctaagaac tcatcagcct
     4741 ccccggtcca tctacccacg taccaatgca ccaattggcc acaatgacgg ctactacatg
     4801 gtgccattcc ttcctcttta taggaatgga gactacctcc tgtccaacaa tgctcttgga
     4861 tacgagtacg cctacctgtt ggacccaggt cattgcacaa caccagaaat gccctctgat
     4921 ctgcaaaaga cgtgaatatc tgttcagaca cccatatcca ctctgttcca cacaggtcag
     4981 aggtttgtcc aggagttctt gacagaggtg taaaaagtac tcaaaaattt tactcaagtg
     5041 aaagtacaag tacttaggga aaattttact caattaaaag taaaagtatc tggctagaat
     5101 cttacttgag taaaagtaaa aaagtactcc attaaaattg tacttgagta ttaaggaagt
     5161 aaaagtaaaa gcaagaaaga aaactagaga ttcttgttta agcttttaat ctcaaaaaac
     5221 attaaatgaa atgcatacaa ggttttatcc tgctttagaa ctgtttgtat ttaattatca
     5281 aactataaga cagacaatct aatgccagta cacgctactc aaagttgtaa aacctcagat
     5341 ttaacttcag tagaagctga ttctcaaaat tgttagtgtc aagcctagct cttttggggc
     5401 tgaaaagcaa tcctgcagtg ctgaaaagcc tctcacaggc agccgatgcg ggaagaggtg
     5461 tattagtctt gatagagagg ctgcaaatag caggaaacgt gagcagagac tccctggtgt
     5521 ctgaaacaca ggccagatgg gccctcgagc aggaaacagc tatgaccatg attacgccaa
     5581 gctatcaact ttgtatagaa aagttggcac gcgcagattc cgtgtgggcc tggtaatatc
     5641 ttaccatttt aagctatttt aagattaggg aaaaaaatta aataaaacta ctttaaaatt
     5701 actttcctta aaaagcaaca tatttattta gtttttgttt aactggctca agattgtaat
     5761 attttacttt tacatgtttt tttaagtacc taaatgtgtt caaaagtaaa gtatgttatt
     5821 gttttttttt tttaatgtta gctaagcttt aaattgtaat atattacttt taaaagtgac
     5881 tttttaaaaa tgtaaataaa aaagtaacta taaatgtgta atgcatgtta tttctgtggg
     5941 tttttaattt tcatctcaac tgggcttaca attttaacat aataaaaatc aaatcaaatc
     6001 aaatcaggcc cttatttgtc acatacactc ttgtatactg aaataaacta aatgttgaga
     6061 aaaatttaaa tacaccaaaa taacgcatac cttttttgta attacctaag taaaacattt
     6121 agttcctttt ttaagtgact caagattgta atctattaat tgtaaaagat aaaactagtg
     6181 atcaatctga cgtgcttcta tgcagcctgt gagattaatt gtgatgttta attattaaac
     6241 aatttaaatg gatcaaacat ttcacatgtg cttattgtgt ccaacaaaac cagcagaagc
     6301 acttaaaaat attgcatgac aacatgagtt tttaatgtaa attatatata cttcctaaac
     6361 gaagttaatc atatttctaa aataataatg tcattgaaat atttaaataa atgataagct
     6421 ttagttgatt caacataaaa ttgaaagcaa taaggacttt ttttttttac agcggtgaag
     6481 caaaagcatg taataatcat ataatataat aaataaataa ataaataatg gctcttggtg
     6541 caaatatgaa cactttaaat aaatatagaa taaatatgat caataagtca taacgatgtg
     6601 ttttatgttg attataattt aaacatgttt ctaacgcaag ttttaaaaac tgttttaaag
     6661 tattatttta agttataaga tcaatttgac tcaacatttt gtagtgaaaa tatgttgtgt
     6721 aaaattaata taatatacac tataaaaatg cagggttcca cacaatacat taatgttgtc
     6781 ccaacacaaa tcgattaagt taacaaatat atgtggattg aacataaaaa attaagttgt
     6841 cccaatgaaa tctcaagaat tgtgttgttt cagctcattt taaataagta gttttaacaa
     6901 gtaaaaaata acatattttt gagtgtgtat atatatatat tacaaaatat aataatataa
     6961 aatgtaaaag atataaacca tgctacatat atttaagagc tattttgcac attacttttt
     7021 tgcaagccat tttagtgttt tttttacaaa tatatattag ctaaatatac aatatcttaa
     7081 atatacaatt aatcagacat gcaattaatg catttaacct acataaaaat acatgatcaa
     7141 catatgtttt atgctgatta caactaaatc aaacgtaatg aaattaattt ataaaagcga
     7201 tttaaatatg tcaatatttt aagttaaaat cacttgaaac ctactataaa tgaacttaaa
     7261 ttaaaaaaat ctcctttaat tccaatgcaa cagtgttgtt ttttcagctg aggacccccg
     7321 gtgcagatgg ttggcccccg gcccgctatt ctaaggaagc ctttcttaat tttagggcgg
     7381 cgctgcatgc gctcagatca gattcacggg tggagagggt tattttctct tttttatttt
     7441 ttcttgcctg ctcatatttg ggcctgtgtg aaccccaggc tccgcctcgg cccccaggag
     7501 ccgctcttat ttaaagggga tcctcgcctt cagtcttcgt cccagtggaa gcatcagtct
     7561 tgtgataaca acttggtgag tcctacagac cagcgctcga ctctaggctt taaatgcatt
     7621 gatttctctg gattagctac cgggagagtt ttaactttgc ttattagtct tgtgctcgaa
     7681 ctgctaggtt taaagagtgt tttctctaat gcatgcatga agcgacagct gaaaaccaga
     7741 aatagagcgt ttgtcctcct gttaaactct agcctttctt tggcatttgt cagtccgagt
     7801 cttcagctgc cggcaggaga ttgggtttca gtaacacttg ctgagcacaa actacacgtg
     7861 ctttcttcag atttaattca ttattaatca ttctagattt aaatgatgtt cttactcatt
     7921 gctgattatt ttagccttgc ggagcagatt aaatgagcac gtgtttcatt aagattgatt
     7981 taaatataat taatatttca ccactgtagt tttaaaattg ccttctaagt catggttgat
     8041 ttttaatagg tatcggctct aattagatta ctattttggt ttttaaatgt tatttctatt
     8101 tcatttgtag gctcgttttt aatgtgattt tattagattc tagttttaaa atcatctctt
     8161 agccttaatg agcagactga atgaacatgt ttcattaaga ttaacttcat tataatgaat
     8221 tatttaatca ttctagtggg ttataaatca gctctattta aattaattgt cgtgtttatt
     8281 tctatttaat ttttgattat tttttcaggg tcgtttttat gtgatttttt cccgtttcta
     8341 attttaaact aattgtttga tttttatatg attcgactct ttttaaatga attgttttgc
     8401 tttttttatt gagcatatat atattataca atttcaatca acaattgttg tgctttttaa
     8461 atgttgtttc tatttcattt ctagggtcga ttttattcga ttctagtttt aaaatcgtga
     8521 tttttaactt ctaaccttac tgagcagact gaatgaacat gtttcatcaa gattaacttc
     8581 attatagtta attattgaat cgtgctagtg gtttataaat cagctctatt taaattaatc
     8641 atcatcatta tttctattta atttttatta tttctttagg attattttta tgtgattttt
     8701 ttcacgttct agttttaaac tcaatgctca ttactgctca cccttgctga gcagtctttc
     8761 gttaagatta aattaattaa taaccattct agttttaaaa cagtgtctta aggcatagct
     8821 gatattttac aagtttacta tttaatatat ctcctttctt tttttagggt cgatttcatc
     8881 tgatttgatt aatttttatt atcttcacta agctttttgt tctaggtttt attctcagta
     8941 ttattactgt tatatttgaa ctgaatttct cctcatagtt tattcatttt atttatttat
     9001 tttttgttta ttcgctattt tgtattttag tttttaatta gaaattttat tagaatcgtt
     9061 ttgaactagt ttttatgcac tctaacatta atattaaatc ataactatta tttttattat
     9121 aatttatttg tgtttatctt tttgtgtagc tttcatgtgt ttttatttta gttttattat
     9181 gtttgcattg aattttttgg actagtttat ataacgatat acagaaaagc catgacaaca
     9241 aatgttttta taatacctat taagtataag ctaatcagat ttccactatt ctgttcaaag
     9301 ttaacattat tagccattct gatggatcat ttgacatttg tcatatgtct ttatgaacac
     9361 tgctctttca gatgccactt ttaaagctgt tttttacagt aaatgaacgt ttattattat
     9421 gacattattt cctaaggcct ctagagtaca gcaggatgtg gcgtgtgcat taccgtgtaa
     9481 ttatgctgac tgtaaatgtg catttataga ctgtcagcat gtctgactgt gcgctgttgt
     9541 gattggtcct gcacagacca ag
//
