LOCUS       sequence-221981-        9552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab8a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9552)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab8a
FEATURES             Location/Qualifiers
     source          1..9552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1376..1396)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1404..1420)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1436..1570
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1460..1479)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1514..1533
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2009..2196,2197..2208)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2242..2258)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2242..2258)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2295..2315)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2295..2313)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2334..2350)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2347..2369)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2358..2374
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2382..2388,2389..2406,2407..2412))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2427..2448
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2565..2582)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2736..3324)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2816..2835)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3495..4286,4287..4355))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4118..4137
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4356..4460)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4623..4644)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(4993..5004,5005..5167)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5540..5556
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5576..5596
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtggcgaag acctacgatt atttgtttaa
      781 actgctgtta atcggggatt ccggagtcgg gaagacctgt gtgctgttca gattctcaga
      841 ggatgccttt aactcgacat ttatctcgac gataggtatc gatttcaaga tcaggacaat
      901 agaactagat ggcaagaaga taaagttaca gatatgggac acggcaggac aggaacgatt
      961 tcggacgatc acgacagcat attacagagg tgcaatgggt attatgctgg tttatgatat
     1021 caccaatgag aagtcatttg acaacatcaa aaactggatc cgaaacatag aggagcatgc
     1081 atcagctgat gtggaaaaga tgattttggg gaacaaatgt gacattaatg agaagaggca
     1141 ggtgtctaaa gacagaggcg agaagctggc gttagagtac ggcatcaagt tcatggagac
     1201 aagtgcaaag gctaatatca acgttgagaa tgctttctta acactcgcca gagacataaa
     1261 agcaaagatg gacacaaaat tggagggaaa caatcctcag ggcagcaacc acggtgtgaa
     1321 aattaccact gaacagccga aaaagagcag cttctttcgc tgtgtgctac tgtgacaact
     1381 ttattataca tagttgataa ttcactggcc gtcgttttac ggtaccatcg atgatgatcc
     1441 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     1501 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     1561 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     1621 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcctct
     1681 agatcagatc tgcgaagata cggccacggg tgctcttgat cctgtggctg attttggact
     1741 gtgctgctcg cagctgctga tgaatcacat acttcctcca ttttcttcca ctgattgact
     1801 gttataattt ccctaatttc caggtcaagg tgctgtgcat tgtggtaata gatgtgacat
     1861 gacgtcactt ccaaaggacc aatgaacatg tctgaccaat ttcatataat gtgaaaacga
     1921 ttttcatagg cagaataaat aacatttaaa ttaaactggg catcagcgca attcaattgg
     1981 tttggtaata gcaagggaaa atagaatgaa gtgatctcca aaaaataagt actttttgac
     2041 tgtaaataaa attgtaagga gtaaaaagta cttttttttc taaaaaaatg taattaagta
     2101 aaagtaaaag tattgatttt taattgtact caagtaaagt aaaaatcccc aaaaataata
     2161 cttaagtaca gtaatcaagt aaaattactc aagtacttta cacctctggt tcttgacccc
     2221 ctaccttcag caagcccagc agatccacta gttctagagc ggccgccacc gcggtggagc
     2281 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
     2341 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     2401 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     2461 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     2521 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     2581 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2641 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     2701 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     2761 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2821 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2881 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     2941 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3001 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3061 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3121 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     3181 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3241 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3301 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3361 cagtggaacg aaaactcacg ttaaggcatt ttggtcatga gattatcaaa aaggatcttc
     3421 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3481 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     3541 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     3601 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     3661 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     3721 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     3781 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     3841 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     3901 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     3961 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     4021 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     4081 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     4141 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     4201 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     4261 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     4321 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     4381 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     4441 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta
     4501 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     4561 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     4621 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     4681 aaaccgtcta tcagggcgat ggcccacgct ggctaagaac tcatcagcct ccccggtcca
     4741 tctacccacg taccaatgca ccaattggcc acaatgacgg ctactacatg gtgccattcc
     4801 ttcctcttta taggaatgga gactacctcc tgtccaacaa tgctcttgga tacgagtacg
     4861 cctacctgtt ggacccaggt cattgcacaa caccagaaat gccctctgat ctgcaaaaga
     4921 cgtgaatatc tgttcagaca cccatatcca ctctgttcca cacaggtcag aggtttgtcc
     4981 aggagttctt gacagaggtg taaaaagtac tcaaaaattt tactcaagtg aaagtacaag
     5041 tacttaggga aaattttact caattaaaag taaaagtatc tggctagaat cttacttgag
     5101 taaaagtaaa aaagtactcc attaaaattg tacttgagta ttaaggaagt aaaagtaaaa
     5161 gcaagaaaga aaactagaga ttcttgttta agcttttaat ctcaaaaaac attaaatgaa
     5221 atgcatacaa ggttttatcc tgctttagaa ctgtttgtat ttaattatca aactataaga
     5281 cagacaatct aatgccagta cacgctactc aaagttgtaa aacctcagat ttaacttcag
     5341 tagaagctga ttctcaaaat tgttagtgtc aagcctagct cttttggggc tgaaaagcaa
     5401 tcctgcagtg ctgaaaagcc tctcacaggc agccgatgcg ggaagaggtg tattagtctt
     5461 gatagagagg ctgcaaatag caggaaacgt gagcagagac tccctggtgt ctgaaacaca
     5521 ggccagatgg gccctcgagc aggaaacagc tatgaccatg attacgccaa gctatcaact
     5581 ttgtatagaa aagttggcac gcgcagattc cgtgtgggcc tggtaatatc ttaccatttt
     5641 aagctatttt aagattaggg aaaaaaatta aataaaacta ctttaaaatt actttcctta
     5701 aaaagcaaca tatttattta gtttttgttt aactggctca agattgtaat attttacttt
     5761 tacatgtttt tttaagtacc taaatgtgtt caaaagtaaa gtatgttatt gttttttttt
     5821 tttaatgtta gctaagcttt aaattgtaat atattacttt taaaagtgac tttttaaaaa
     5881 tgtaaataaa aaagtaacta taaatgtgta atgcatgtta tttctgtggg tttttaattt
     5941 tcatctcaac tgggcttaca attttaacat aataaaaatc aaatcaaatc aaatcaggcc
     6001 cttatttgtc acatacactc ttgtatactg aaataaacta aatgttgaga aaaatttaaa
     6061 tacaccaaaa taacgcatac cttttttgta attacctaag taaaacattt agttcctttt
     6121 ttaagtgact caagattgta atctattaat tgtaaaagat aaaactagtg atcaatctga
     6181 cgtgcttcta tgcagcctgt gagattaatt gtgatgttta attattaaac aatttaaatg
     6241 gatcaaacat ttcacatgtg cttattgtgt ccaacaaaac cagcagaagc acttaaaaat
     6301 attgcatgac aacatgagtt tttaatgtaa attatatata cttcctaaac gaagttaatc
     6361 atatttctaa aataataatg tcattgaaat atttaaataa atgataagct ttagttgatt
     6421 caacataaaa ttgaaagcaa taaggacttt ttttttttac agcggtgaag caaaagcatg
     6481 taataatcat ataatataat aaataaataa ataaataatg gctcttggtg caaatatgaa
     6541 cactttaaat aaatatagaa taaatatgat caataagtca taacgatgtg ttttatgttg
     6601 attataattt aaacatgttt ctaacgcaag ttttaaaaac tgttttaaag tattatttta
     6661 agttataaga tcaatttgac tcaacatttt gtagtgaaaa tatgttgtgt aaaattaata
     6721 taatatacac tataaaaatg cagggttcca cacaatacat taatgttgtc ccaacacaaa
     6781 tcgattaagt taacaaatat atgtggattg aacataaaaa attaagttgt cccaatgaaa
     6841 tctcaagaat tgtgttgttt cagctcattt taaataagta gttttaacaa gtaaaaaata
     6901 acatattttt gagtgtgtat atatatatat tacaaaatat aataatataa aatgtaaaag
     6961 atataaacca tgctacatat atttaagagc tattttgcac attacttttt tgcaagccat
     7021 tttagtgttt tttttacaaa tatatattag ctaaatatac aatatcttaa atatacaatt
     7081 aatcagacat gcaattaatg catttaacct acataaaaat acatgatcaa catatgtttt
     7141 atgctgatta caactaaatc aaacgtaatg aaattaattt ataaaagcga tttaaatatg
     7201 tcaatatttt aagttaaaat cacttgaaac ctactataaa tgaacttaaa ttaaaaaaat
     7261 ctcctttaat tccaatgcaa cagtgttgtt ttttcagctg aggacccccg gtgcagatgg
     7321 ttggcccccg gcccgctatt ctaaggaagc ctttcttaat tttagggcgg cgctgcatgc
     7381 gctcagatca gattcacggg tggagagggt tattttctct tttttatttt ttcttgcctg
     7441 ctcatatttg ggcctgtgtg aaccccaggc tccgcctcgg cccccaggag ccgctcttat
     7501 ttaaagggga tcctcgcctt cagtcttcgt cccagtggaa gcatcagtct tgtgataaca
     7561 acttggtgag tcctacagac cagcgctcga ctctaggctt taaatgcatt gatttctctg
     7621 gattagctac cgggagagtt ttaactttgc ttattagtct tgtgctcgaa ctgctaggtt
     7681 taaagagtgt tttctctaat gcatgcatga agcgacagct gaaaaccaga aatagagcgt
     7741 ttgtcctcct gttaaactct agcctttctt tggcatttgt cagtccgagt cttcagctgc
     7801 cggcaggaga ttgggtttca gtaacacttg ctgagcacaa actacacgtg ctttcttcag
     7861 atttaattca ttattaatca ttctagattt aaatgatgtt cttactcatt gctgattatt
     7921 ttagccttgc ggagcagatt aaatgagcac gtgtttcatt aagattgatt taaatataat
     7981 taatatttca ccactgtagt tttaaaattg ccttctaagt catggttgat ttttaatagg
     8041 tatcggctct aattagatta ctattttggt ttttaaatgt tatttctatt tcatttgtag
     8101 gctcgttttt aatgtgattt tattagattc tagttttaaa atcatctctt agccttaatg
     8161 agcagactga atgaacatgt ttcattaaga ttaacttcat tataatgaat tatttaatca
     8221 ttctagtggg ttataaatca gctctattta aattaattgt cgtgtttatt tctatttaat
     8281 ttttgattat tttttcaggg tcgtttttat gtgatttttt cccgtttcta attttaaact
     8341 aattgtttga tttttatatg attcgactct ttttaaatga attgttttgc tttttttatt
     8401 gagcatatat atattataca atttcaatca acaattgttg tgctttttaa atgttgtttc
     8461 tatttcattt ctagggtcga ttttattcga ttctagtttt aaaatcgtga tttttaactt
     8521 ctaaccttac tgagcagact gaatgaacat gtttcatcaa gattaacttc attatagtta
     8581 attattgaat cgtgctagtg gtttataaat cagctctatt taaattaatc atcatcatta
     8641 tttctattta atttttatta tttctttagg attattttta tgtgattttt ttcacgttct
     8701 agttttaaac tcaatgctca ttactgctca cccttgctga gcagtctttc gttaagatta
     8761 aattaattaa taaccattct agttttaaaa cagtgtctta aggcatagct gatattttac
     8821 aagtttacta tttaatatat ctcctttctt tttttagggt cgatttcatc tgatttgatt
     8881 aatttttatt atcttcacta agctttttgt tctaggtttt attctcagta ttattactgt
     8941 tatatttgaa ctgaatttct cctcatagtt tattcatttt atttatttat tttttgttta
     9001 ttcgctattt tgtattttag tttttaatta gaaattttat tagaatcgtt ttgaactagt
     9061 ttttatgcac tctaacatta atattaaatc ataactatta tttttattat aatttatttg
     9121 tgtttatctt tttgtgtagc tttcatgtgt ttttatttta gttttattat gtttgcattg
     9181 aattttttgg actagtttat ataacgatat acagaaaagc catgacaaca aatgttttta
     9241 taatacctat taagtataag ctaatcagat ttccactatt ctgttcaaag ttaacattat
     9301 tagccattct gatggatcat ttgacatttg tcatatgtct ttatgaacac tgctctttca
     9361 gatgccactt ttaaagctgt tttttacagt aaatgaacgt ttattattat gacattattt
     9421 cctaaggcct ctagagtaca gcaggatgtg gcgtgtgcat taccgtgtaa ttatgctgac
     9481 tgtaaatgtg catttataga ctgtcagcat gtctgactgt gcgctgttgt gattggtcct
     9541 gcacagacca ag
//
